Structural properties of starch-chitosan-gelatin foams and the impact of gelatin on MC3T3 mouse osteoblast cell viability

Abstract

BackgroundThis study examines the effects of adding gelatin to a starch-chitosan composite foam, focusing on the altered structural and biological properties. The compressive modulus of foams containing different gelatin concentrations was tested in dry, wet, and lyophilized states. MC3T3 mouse osteoblast cells were used to test the composite’s ability to support cell growth. The stability of the foams in α-MEM culture media with and without cells was also examined.ResultsIt was found that for dry foams, the compressive modulus increased with increasing gelatin content. For foams tested in wet and lyophilized states, the compressive modulus peaked at a gelatin concentration of 2.5% and 5%, respectively. The growth of MC3T3 mouse osteoblast cells was tested on the foams with different gelatin concentrations. The addition of gelatin had a positive effect on the cell growth and proliferation.ConclusionThe composite foam containing gelatin improved cell growth and is only dissolved by the growing cells at a rate influenced by the initial concentration of gelatin added to the foam.

Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering

“…The incorporation of gelatin in other polymers is one of the strategies to improve their biological activity. For instance, Risser et al () showed that increasing concentration of gelatin in starch‐chitosan‐gelatin composite foams have a positive effect on the growth and proliferation of MC3T3 mouse osteoblast cells. In turn, the incorporation of gelatin in PVA electrospun nanofibers significantly improved the adhesion, spreading, and flattening of the 3T3 mouse fibroblasts (Huang et al, ).…”

“…In order to overcome its limitations including high degradation rate in aqueous environment and weak mechanical properties, chemical crosslinking agents, such as formaldehyde, glyoxal, glutaraldehyde, genipin, and transglutaminase, are used (Pulat & Akalin, ; Xing et al, ). Since, gelatin contains Arg‐Gly‐Asp (RGD) sequences that promote cell adhesion and spreading, it has been blended with other polymers (e.g., chitosan, alginate, poly(vinyl alcohol) ‐ PVA, and starch/chitosan) to obtain hydrogels with improved biological activity (Huang, Hu, & Wei, ; Maji, Dasgupta, Pramanik, & Bissoyi, ; Risser, Banik, Brown, & Catchmark, ; Sarker et al, ).…”

Desenvolvimento e caracterização de membrana de gelatina/quitosana com nanopartículas de prata

“…Com isso, a eficácia dessa blenda foi estudada por diversos pesquisadores, comoCovarrubias et al, (2018), que desenvolveram um scaffold com quitosana e gelatina contendo nanopartículas de biovidro, o que resultou em um biomaterial com capacidade de acelerar a recuperação óssea. Assim comoRisser et al, (2017), que analisaram o efeito biológico de uma espuma de amido e quitosana com gelatina, em células osteoblásticas, e observaram um crescimento e proliferação celular proporcional à concentração de gelatina na amostra.Dessa forma, a blenda de quitosana e gelatina apresenta algumas vantagens, como aumento na permeação ao vapor, potencializa as propriedades que influenciam a atividade biológica, como melhora da biocompatibilidade, presença de aminoácidos que promovem adesão e migração celular, pois tanto a quitosana como a gelatina influenciam o meio celular…”