Structural properties of microheterogeneous surfactant-based catalytic systems: Multicomponent self-diffusion NMR approach

Zuev, Yu. F.; Mirgorodskaya, A. B.; Idiyatullin, B. Z.

doi:10.1007/bf03166745

Cited by 9 publications

(3 citation statements)

References 26 publications

(33 reference statements)

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“…Water is assumed to regulate the partition of butanol between phases, retaining constant its concentration in the dispersion phase, which is supported by the data of NMR self -diffusion. 27 This decreases the content of butanol at the interface increasing the positive charge density of aggregates and the surface concentration of hydroxide -ions.…”

Section: The Hydrolysis Of Carbonic Acid Esters In Microemulsions Undmentioning

confidence: 99%

Reactions in Supramolecular Systems

Zakharova

Mirgorodskaya

Zhil’tsova

et al. 2010

Molecular Encapsulation

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Section: The Hydrolysis Of Carbonic Acid Esters In Microemulsions Undmentioning

confidence: 99%

Reactions in Supramolecular Systems

Zakharova

Mirgorodskaya

Zhil’tsova

et al. 2010

Molecular Encapsulation

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“…However, the most common approach to enzyme protection is immobilization on different carriers. Immobilized enzymes have been widely used in industry since the 1960s [19,20], but the selection of an immobilization matrix has traditionally been carried out through empirical methods. This approach hampers the efficient utilization of new biocatalysts in industrial production and the accumulation of theoretical knowledge regarding activity alterations in different microenvironments and enzyme interactions with immobilization matrices.…”

Section: Introductionmentioning

confidence: 99%

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Сорокин

Goncharova

Lavlinskaya

et al. 2023

IJMS

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This study investigates the features of interactions between cysteine proteases (bromelain, ficin, and papain) and a graft copolymer of carboxymethyl cellulose sodium salt with N-vinylimidazole. The objective is to understand the influence of this interactions on the proteolytic activity and stability of the enzymes. The enzymes were immobilized through complexation with the carrier. The interaction mechanism was examined using Fourier-transform infrared spectroscopy and flexible molecular docking simulations. The findings reveal that the enzymes interact with the functional groups of the carrier via amino acid residues, resulting in the formation of secondary structure elements and enzyme’s active sites. These interactions induce modulation of active site of the enzymes, leading to an enhancement in their proteolytic activity. Furthermore, the immobilized enzymes demonstrate superior stability compared to their native counterparts. Notably, during a 21-day incubation period, no protein release from the conjugates was observed. These results suggest that the complexation of the enzymes with the graft copolymer has the potential to improve their performance as biocatalysts, with applications in various fields such as biomedicine, pharmaceutics, and biotechnology.

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“…In some cases, PFG is combined with NMRspectroscopy to further differentiate between species in a mixture. − In recent years, benchtop NMR has gained many users due to various factors. Not only do they have lower price and involve null maintenance costs but their operation modes are also easy that do not require an NMR expert and special laboratory requirements.…”

Section: Introductionmentioning

confidence: 99%

Species Distribution in Bicontinuous Phase Systems for Enhanced Oil Recovery Probed by Single-Sided NMR

Velasco

Iborra

Giussi³

et al. 2022

Langmuir

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Multiphase aqueous-organic systems where a bicontinuous phase is in equilibrium with an excess organic and aqueous phase find various applications in industry. These systems�also known as Winsor III�are complex not only for the different phases that develop therein but also because they are multicomponent systems. In this work, we explore for the first time the use of a benchtop low-field single-sided NMR to determine the species distribution in Winsor III systems. The proposed methodology provides information at macroscopic and microscopic levels. In particular, we show the use of single-sided NMR to determine the phases' dimensions and the species distribution in a polymer-based bicontinuous system. The phases' dimensions and limits can be resolved with micrometric precision and are indicative of the bicontinuous phase stability. The species distribution is determined by means of spatially resolved NMR relaxation and diffusion experiments. It was observed that the salinity of the aqueous phase also impacts the species distribution in the bicontinuous system. Experiments show that the additive and the polymer are mainly located in the bicontinuous phase. As the salinity of the aqueous phase increases, the amount of organic components in the bicontinuous phase decreases as a consequence of the species distribution in the system. This influences the total amount of recovered organic liquid from the organic phase. The information is obtained in a relatively fast experiment and is relevant to the system's possible applications, such as enhanced oil recovery (EOR). This methodology is not only circumscribed to its application in EOR but can also be applied to the study of any emulsion or microemulsion systems without sample size or geometry constraints.

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Structural properties of microheterogeneous surfactant-based catalytic systems: Multicomponent self-diffusion NMR approach

Cited by 9 publications

References 26 publications

Reactions in Supramolecular Systems

Reactions in Supramolecular Systems

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Species Distribution in Bicontinuous Phase Systems for Enhanced Oil Recovery Probed by Single-Sided NMR

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