Structural Properties of Fractionated Starch Polymers and Their Dependence on the Dissolution Process

Abstract

A combination of sedimentation field flow fractionation (SdFFF) with multiangle laser light scattering (MALLS) was applied to the grain level destructured potato and waxy corn starch in solution.The molar mass and radius distributions were obtained simultaneously. Separation was observed for a radii range of 50-500 nm. Degradation upon heat exposure was found depending mainly on the exposure time and less on the temperature. A detailed analysis of the particle scattering factors (angular dependence of the scattered light) revealed small differences in the internal structure of potato and waxy corn starches. Both starches showed the behavior of only weakly swollen, branched clusters. Potato starch appeared to be slightly more expanded than waxy corn starch. Self-similar structures were observed with fractal dimensions of 2.4 ( 0.1. The deviations from theoretical predictions using the mean field approximation could result from excluded volume effects and heterogeneity in branching.

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

“…(3) was used by Dias [20] and Dias et al [38] to separate amylose and amylopectin -by PCHDC -present in different commercial starches (gifts from National Starch & Chemical). The large molecules of amylopectin [42][43][44][45][46][47][48][49][50] eluted first and the resolution was similar to that obtained with size exclusion chromatography.…”

Macromolecular characteristics of ten yam (Dioscorea spp) starches

“…21,24 The presence of branching was expected to decrease the ν value. As experimental ν values in the 0.40-0.45 range have been obtained for amylopectin, 12,20,22 the ν G values obtained for yam starches by integrating the total LS peak were low compared with data in the literature.…”

“…These values were in good agreement with data in the literature. 12,20,22 The lowest ν G values (∼0.36) were observed for the maize starch and esculenta 5 (D esculenta) and dumetorum (D dumetorum) yam species starches. Thus, these amylopectins were the densest ones and probably had a high degree of branching compared with the other amylopectins studied.…”

Light Scattering Techniques

“…Calibration curve obtained with a DEAE-dextran using a 0·8% NaNG3 aqueous solution as eluent. 60 The peculiar increase of the curve at high elution volumes is due to specific interactions with the gel.…”