The search for sources of animal feed has been well-regarded for farmers. In spite of this, the objective of this study was to show the thermal, physical and chemical properties of earthworms flour from Eisenia andrei for supplementary food for animals. The species was studied by simultaneous thermogravimetric analysis, differential scanning calorimetry, combustion calorimetry, moisture, ash, fat, protein, carbohydrate and fibre analysis. The samples were dried, ground, screened and stored in suitable vials. After the analysis of physical and chemical characterization, it was possible to verify that E. andrei flour presents higher calorific power and levels of protein, fiber, carbohydrate, essential metals and low fat. It can be a promising product as an ideal alternative protein source for better growth performance of animals.