1976
DOI: 10.1016/0022-2836(76)90294-1
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Structural organization of the genome of Dictyostelium discoideum: Analysis by EcoR1 restriction endonuclease

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Cited by 67 publications
(24 citation statements)
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“…The relatively high copy number of the A6NPTII vector is consistent with the observation of a vector-sized band in ethidium bromide-stained gels of EcoRI-digested DNA from A6NPTII transformants (solid arrowhead, top right panel). The intensity of the vector band was comparable to the intensity of the flanking EcoRI fragments of rDNA, which are present at several hundred copies per haploid genome (18) with other clonal isolates and with populations of transformants, indicating that the vector copy number in transformed cells is a vector-specific property.…”
Section: Resultsmentioning
confidence: 67%
“…The relatively high copy number of the A6NPTII vector is consistent with the observation of a vector-sized band in ethidium bromide-stained gels of EcoRI-digested DNA from A6NPTII transformants (solid arrowhead, top right panel). The intensity of the vector band was comparable to the intensity of the flanking EcoRI fragments of rDNA, which are present at several hundred copies per haploid genome (18) with other clonal isolates and with populations of transformants, indicating that the vector copy number in transformed cells is a vector-specific property.…”
Section: Resultsmentioning
confidence: 67%
“…Late-logarithmic phase T. brucei genomic DNA was extracted as described (23) and analyzed by the method of Southern (24). The DNA was resolved by electrophoresis on 0.6% agarose gels, transferred onto a GeneScreenPlus membrane, and hybridized to DNA probes radiolabeled by random priming (25).…”
Section: Methodsmentioning
confidence: 99%
“…The majority of the labeled and unlabeled fragments were purified by agarose gel electrophoreses. Restriction endonuclease reactions were stopped with gel loading buffer and loaded directly on a flat-bed agarose gel containing 1 ,ug of ethidium bromide per ml and run as described (13). The mobility of the fragment was visualized during the run with short wavelength UV light.…”
Section: Observations)mentioning
confidence: 99%