Structural Organization and Mapping of the Human TCF11 Gene

Luna, Luisa; Skammelsrud, Nina; Johnsen, Øyvind; Abel, Kenneth; Weber, Barbara; Prydz, Hans; Kolstø, Anne‐Brit

doi:10.1006/geno.1995.1037

Cited by 37 publications

(36 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mapping of the 5h terminus of the hbl mRNA was performed on total RNA isolated from B. cereus ATCC 14579, using two different 5h end-labelled oligonucleotides, P1 (5h-TCC TTT CTG TCT GGT CAT ACC-3h) and P2 (5h-TCA GCA AAC TCC TTA CTA GAC-3h), located 580 and 363 bp upstream of the putative translational start site of hblC, respectively. Annealing and extension were performed according to Luna et al (1994) using 10 U AMV Reverse Transcriptase (Promega) in the extension reaction. Products were resolved in 6 % polyacrylamide gels together with sequence reactions prepared according to the dideoxy chain-termination method using T7 DNA polymerase (Pharmacia).…”

Section: Methodsmentioning

confidence: 99%

Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes

et al. 1999

Self Cite

View full text Add to dashboard Cite

Haemolysin BL (HBL) is a Bacillus cereus toxin composed of a binding component, B, and two lytic components, L 1 and L 2 . HBL is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of B. cereus. The three genes encoding the HBL components constitute an operon and are transcribed from a promoter 608 bp upstream of the hblC translational start site. The first gene of the hbl operon, hblC, in the B. cereus type strain, ATCC 14579, was inactivated in this study. Inactivation of hblC strongly reduced both the enterotoxigenic activity of B. cereus ATCC 14579 and the haemolytic activity against sheep erythrocytes, while maintaining full haemolytic activity against human erythrocytes.

show abstract

Section: Methodsmentioning

confidence: 99%

Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes

et al. 1999

Self Cite

View full text Add to dashboard Cite

show abstract

“…In silico analysis of this promoter variant using the program MatInspector revealed a potential binding site for heterodimers of TCF-11 (a transcription factor of the bzip type 27,28 ) and MafG (a member of the Maf subfamily of the transcription factor AP-1 29 ) when there was a T at the position of the polymorphism. Noteworthy, AP-1 is known to play a key role in the regulation of IL2 expression.…”

Section: Il2 Association Analysismentioning

confidence: 99%

Family based association analysis of the IL2 and IL15 genes in allergic disorders

et al. 2005

View full text Add to dashboard Cite

Allergic diseases affect an increasing number of individuals and are a major global health problem. A substantial genetic contribution in the aetiology of allergic diseases is well documented. We have previously reported linkage of allergic diseases and atopy to the region harbouring the IL2 gene (4q27). IL15 is located approximately 20 Mb distal to IL2. The two genes encode cytokines that are structurally and functionally related, both inducing T-cell activation and proliferation. We screened the two genes for sequence variation and applied the seven single-nucleotide polymorphisms (SNPs) identified in a family based association study of two Danish samples comprising a total of 235 families with allergic diseases. None of the IL15 SNPs showed significant association and the haplotype analysis yielded inconsistent results in the two samples. In contrast, the two IL2 SNPs showed association both separately and in haplotypes with several atopic phenotypes, most significantly with IgE-mediated allergy. (single SNP P-value 0.0005 for positive skin prick test, haplotype P-value 0.019 for positive RAST test). To our knowledge, this is the first study reporting association between IL2 and IgE-mediated allergy, asthma and atopic eczema. The SNP (rs2069762) that showed the most consistent results is located in the promoter and has previously been shown to influence the level of IL2 expression. We suggest that the observed overtransmission of the T allele of this SNP may convey increased susceptibility to allergic disease by skewing the Th1/Th2 balance towards Th2.

show abstract

Section: Introductionmentioning

confidence: 99%

“…TCF11, consisting of 772 amino acids, is the longest form of Nrf1 [44]. Nrf1a (742 aa) is generated through alternate splicing of exon 4 of the Nrf1 gene and lacks a Neh4 subdomain (aa 242-271 of TCF11) that contains a transactivation domain [44]. TCF11 and Nrf1a are anchored within the endoplasmic reticulum membrane through their N-terminal domain, and they undergo retro-translocation into the cytoplasm and processing prior to entry into the nucleus to promote gene expression [45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) is regulated by O-GlcNAc transferase

Han

Valdez

et al. 2017

Free Radical Biology and Medicine

View full text Add to dashboard Cite

A B S T R A C TThe Nrf1 (Nuclear factor E2-related factor 1) transcription factor performs a critical role in regulating cellular homeostasis. Using a proteomic approach, we identified Host Cell Factor-1 (HCF1), a co-regulator of transcription, and O-GlcNAc transferase (OGT), the enzyme that mediates protein O-GlcNAcylation, as cellular partners of Nrf1a, an isoform of Nrf1. Nrf1a directly interacts with HCF1 through the HCF1 binding motif (HBM), while interaction with OGT is mediated through HCF1. Overexpression of HCF1 and OGT leads to increased Nrf1a protein stability. Addition of O-GlcNAc decreases ubiquitination and degradation of Nrf1a. Transcriptional activation by Nrf1a is increased by OGT overexpression and treatment with PUGNAc. Together, these data suggest that OGT can act as a regulator of Nrf1a.

show abstract

Structural Organization and Mapping of the Human TCF11 Gene

Cited by 37 publications

References 0 publications

Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes

Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes

Family based association analysis of the IL2 and IL15 genes in allergic disorders

Nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) is regulated by O-GlcNAc transferase

Contact Info

Product

Resources

About