Structural Organization and Fine Distribution of Lymphatic Vessels in Periodontal Tissues of the Rat and Monkey: A Histochemical Study

Zhang, Bi; Miura, Masahiro; Ji, Rui-Cheng; Matsumoto, Yuushi; Kato, Seiji

doi:10.2535/ofaj1936.77.4_93

Cited by 7 publications

(11 citation statements)

References 22 publications

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“…5Ј-Nase is known to be activated by the supply of Mg ϩϩ ions (Center and Behal, 1966). In particular, we have demonstrated the enhancement of 5Ј-Nase activity in the monkey lymphatic endothelium by the immersion of the specimens in a MgCl 2 solution after decalcification of the tissue using EDTA (Zhang et al, 2000). Recently, Nara et al (2000) demonstrated the reactivation of 5Ј-Nase by the supply Mg ϩϩ ions in the hamster periodontium using BEI-SEM histochemistry.…”

Section: Histochemical Reactivity Of the Lymphatics Inmentioning

confidence: 99%

“…Microwave irradiation shortened the decalcification time during EDTA treatment from 2 weeks to about 4 days without decreasing the 5Ј-Nase and ALPase activities. The decalcification procedure involving EDTA, however, inhibits the activity of 5Ј-Nase, even when the incubation period is reduced by exposing the specimens to microwave irradiation (Matsumoto et al, 1997;Zhang et al, 2000). 5Ј-Nase is known to be activated by the supply of Mg ϩϩ ions (Center and Behal, 1966).…”

Section: Histochemical Reactivity Of the Lymphatics Inmentioning

confidence: 99%

“…5Ј-Nase and ALPase are both known to be activated by the Mg ϩϩ ion (Center and Behal, 1966;Yoshiki et al, 1972). In the incubation of the decalcified specimen with MgCl 2 disolution, the decrease in 5Ј-Nase activity by decalcification was improved by the Mg ϩϩ ion supply (Nara et al, 2000;Zhang et al, 2000). In this manner, the microscopic characteristics and fine distribution of the lymphatic vessels in the periodontal tissues and dental pulp of animals and human can be examined with or without decalcification using our enzyme-histochemical approach.…”

Section: Introduction: Historical Backgroundmentioning

confidence: 99%

“…Previously, the existence of lymphatic vessels in the dental pulp and periodontal tissue has been demonstrated histochemically (Aoyama et al, 1995;Matsumoto et al, 1997;Zhang et al, 2000) using 5Ј-nucleotidase (5Ј-Nase)-alkaline phosphatase (ALPase) double staining (Kato and Miyauchi, 1989;Kato et al, 1993). This method is useful for identifying lymphatic vessels and determining their fine distribution, with special reference to organ specificity (Kato, 2000).…”

Section: Introduction: Historical Backgroundmentioning

confidence: 99%

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Lymphatic networks in the periodontal tissue and dental pulp as revealed by histochemical study

Matsumoto

Zhang

Kato

2002

Microscopy Res & Technique

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The structural organization and fine distribution of the lymphatic networks in the periodontal tissues (gingiva, periodontal membrane, and alveolar process) and dental pulp of animals and humans were reviewed with special reference to histochemical examination by light and electron microscopy. The distinction between lymphatics and blood vessels was made on cryostat sections of undecalcified and calcified teeth treated with EDTA solution and whole mount preparations of periodontal membranes using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining. This staining procedure allowed lymphatic vessels in the periodontal tissue and dental pulp to be differentiated from blood vessels. The specificity and localization of the enzyme reactions were confirmed by comparative histochemical studies of the same specimen with light microscopy and scanning or transmission electron microscopy. Well-developed 5'-Nase-positive lymphatic networks were observed on the tissue sections and whole mount preparations of the gingiva, periodontium, and dental pulp. More lymphatic vessels were seen in the root area of the periodontium than in the cervical area. In the dental pulp, lymphatic vessels were more numerous in the central part than in the peripheral odontoblastic layer. These distributions of the lymphatic capillary networks are discussed in relation to their ability to supply lymph to the teeth.

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Section: Histochemical Reactivity Of the Lymphatics Inmentioning

confidence: 99%

Section: Histochemical Reactivity Of the Lymphatics Inmentioning

confidence: 99%

Section: Introduction: Historical Backgroundmentioning

confidence: 99%

Section: Introduction: Historical Backgroundmentioning

confidence: 99%

See 2 more Smart Citations

Lymphatic networks in the periodontal tissue and dental pulp as revealed by histochemical study

Matsumoto

Zhang

Kato

2002

Microscopy Res & Technique

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“…In human healthy and inflamed dental pulps, Marchetti et al (1991Marchetti et al ( , 1992 demonstrated that a well-developed lymphatic network does exist, as wide intracellular channels. Recently, the fine distribution of lymphatics has been demonstrated histochemically in human dental pulp (Matsumoto et al 1997) and rat and monkey periodontal tissues (Zhang et al, 2000) using 5Ј-nucleotidase-alkaline phosphatase double staining (Kato and Miyauchi, 1989). Lymphatics were found in all regions of the dental pulp except the odontoblast layer and the dental horns under the cusps.…”

Section: Introductionmentioning

confidence: 99%

Introduction

Kato

2002

Microscopy Res & Technique

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Effect of VEGFC on lymph flow and inflammation‐induced alveolar bone loss

Wang

Chen

et al. 2020

The Journal of Pathology

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The lymphatic system plays a crucial role in the maintenance of tissue fluid homeostasis and the immunological response to inflammation. The effects of lymphatic drainage dysfunction on periodontitis have not been well studied. Here we show that lymphatic vessel endothelial receptor 1 (LYVE1)+/podoplanin (PDPN)+ lymphatic vessels (LVs) are increased in the periodontal tissues, with accumulation close to the alveolar bone surface, in two murine periodontitis models: rheumatoid arthritis (RA)‐associated periodontitis and ligature‐induced periodontitis. Further, PDPN+/alpha‐smooth muscle actin (αSMA)− lymphatic capillaries are increased, whereas PDPN+/αSMA+ collecting LVs are decreased significantly in the inflamed periodontal tissues. Both mouse models of periodontitis have delayed lymph flow in periodontal tissues, increased TRAP‐positive osteoclasts, and significant alveolar bone loss. Importantly, the local administration of adeno‐associated virus for vascular endothelial growth factor C, the major growth factor that promotes lymphangiogenesis, increases the area and number of PDPN+/αSMA+ collecting LVs, promotes local lymphatic drainage, and reduces alveolar bone loss in both models of periodontitis. Lastly, LYVE1+/αSMA− lymphatic capillaries are increased, whereas LYVE1+/αSMA+ collecting LVs are decreased significantly in gingival tissues of patients with chronic periodontitis compared with those of clinically healthy controls. Thus, our findings reveal an important role of local lymphatic drainage in periodontal inflammation‐mediated alveolar bone loss. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

show abstract

Structural Organization and Fine Distribution of Lymphatic Vessels in Periodontal Tissues of the Rat and Monkey: A Histochemical Study

Cited by 7 publications

References 22 publications

Lymphatic networks in the periodontal tissue and dental pulp as revealed by histochemical study

Lymphatic networks in the periodontal tissue and dental pulp as revealed by histochemical study

Introduction

Effect of VEGFC on lymph flow and inflammation‐induced alveolar bone loss

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