Structural, optical and potential sensing properties of tyrosinase immobilized graphene oxide thin film on gold surface

Hashim, Hazwani Suhaila; Fen, Yap Wing; Omar, Nur Alia Sheh; Daniyal, ‬Wan Mohd Ebtisyam Mustaqim Mohd; Saleviter, Silvan; Abdullah, Jaafar

doi:10.1016/j.ijleo.2020.164786

Cited by 16 publications

(12 citation statements)

References 98 publications

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“…The analytical ranges of the developed procedures allowed phenol determination in waste waters, l -tyrosine determination in food supplements, and l -DOPA determination in biological fluids. LOD values are comparable with other spectroscopic procedures using purified tyrosinase [ 15 , 44 , 45 , 46 , 47 , 48 ] ( Table 7 ). For l -tyrosine, no spectroscopic procedures were found in the literature; however, the comparison with electrochemical determination (LOD 1.0 × 10 −5 M [ 49 ]) allows us to conclude the same.…”

Section: Resultssupporting

confidence: 79%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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In the present work crude Agaricus bisporus extract (ABE) has been prepared and characterized by its tyrosinase activity, protein composition and substrate specificity. The presence of mushroom tyrosinase (PPO3) in ABE has been confirmed using two-dimensional electrophoresis, followed by MALDI TOF/TOF MS-based analysis. GH27 alpha-glucosidases, GH47 alpha-mannosidases, GH20 hexosaminidases, and alkaline phosphatases have been also detected in ABE. ABE substrate specificity has been studied using 19 phenolic compounds: polyphenols (catechol, gallic, caffeic, chlorogenic, and ferulic acids, quercetin, rutin, dihydroquercetin, l-dihydroxyphenylalanine, resorcinol, propyl gallate) and monophenols (l-tyrosine, phenol, p-nitrophenol, o-nitrophenol, guaiacol, o-cresol, m-cresol, p-cresol). The comparison of ABE substrate specificity and affinity to the corresponding parameters of purified A. bisporus tyrosinase has revealed no major differences. The conditions for spectrophotometric determination have been chosen and the analytical procedures for determination of 1.4 × 10−4–1.0 × 10−3 M l-tyrosine, 3.1 × 10−6–1.0 × 10−4 M phenol, 5.4 × 10−5–1.0 × 10−3 M catechol, 8.5 × 10−5–1.0 × 10−3 M caffeic acid, 1.5 × 10−4–7.5 × 10−4 M chlorogenic acid, 6.8 × 10−5–1.0 × 10−3 M l-DOPA have been proposed. The procedures have been applied for the determination of l-tyrosine in food supplements, l-DOPA in synthetic serum, and phenol in waste water from the food manufacturing plant. Thus, we have demonstrated the possibility of using ABE as a substitute for tyrosinase in such analytical applications, as food supplements, medical and environmental analysis.

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Section: Resultssupporting

confidence: 79%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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“…Before the composite solution was coated onto the glass slip, the glass slip was first deposited with gold layer. The glass cover slip was deposited with a gold layer by using an SC7640 Sputter Coater (Quorum Technologies, West Sussex, UK) with the duration of 67 s for the sputtering process to obtain a 50 nm thick gold layer, which is the optimum thickness for an SPR sensor [ 93 ].…”

Section: Methodsmentioning

confidence: 99%

X-ray Photoelectron Spectroscopy Analysis of Chitosan–Graphene Oxide-Based Composite Thin Films for Potential Optical Sensing Applications

et al. 2021

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In this study, X-ray photoelectron spectroscopy (XPS) was used to study chitosan–graphene oxide (chitosan–GO) incorporated with 4-(2-pyridylazo)resorcinol (PAR) and cadmium sulfide quantum dot (CdS QD) composite thin films for the potential optical sensing of cobalt ions (Co2+). From the XPS results, it was confirmed that carbon, oxygen, and nitrogen elements existed on the PAR–chitosan–GO thin film, while for CdS QD–chitosan–GO, the existence of carbon, oxygen, cadmium, nitrogen, and sulfur were confirmed. Further deconvolution of each element using the Gaussian–Lorentzian curve fitting program revealed the sub-peak component of each element and hence the corresponding functional group was identified. Next, investigation using surface plasmon resonance (SPR) optical sensor proved that both chitosan–GO-based thin films were able to detect Co2+ as low as 0.01 ppm for both composite thin films, while the PAR had the higher binding affinity. The interaction of the Co2+ with the thin films was characterized again using XPS to confirm the functional group involved during the reaction. The XPS results proved that primary amino in the PAR–chitosan–GO thin film contributed more important role for the reaction with Co2+, as in agreement with the SPR results.

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“…(64)(65)(66)(67)(68)(69) Exposure to phenol over its permissible level can lead to symptoms such as vomiting and diarrhea, and further exposure can cause kidney, lung, and liver malfunction. (70)(71)(72)(73)(74)(75) Consequently, it has drawn researchers' attention, leading to the rapid development of sensors for its detection. SPR sensors have emerged as novel sensors with high potential for detecting phenol.…”

Section: Phenol Detectionmentioning

confidence: 99%