Structural, morphometric and immunohistochemical study of the rabbit accessory olfactory bulb

Villamayor, Paula R.; Cifuentes, Manuel; Arias, Luis Angel Quintela; Barcia, Ramiro; Sánchez‐Quinteiro, Pablo

doi:10.1007/s00429-019-01997-4

Cited by 25 publications

(46 citation statements)

References 107 publications

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“…With regard to the morphology of the AOB, our observations in the newborn capybara show that from birth, the macroscopic and microscopic organisation of the AOB are already defined, showing that the AOB of the capybara has a differentiated laminar pattern (Fig. 7 ), similar to that found in other rodents 39 , lagomorphs, marsupials and prosimians 72 – 74 . From the macroscopic point of view, it is remarkable that in the specimens studied by Suárez et al 23 , the arrival of the VNN to the AOB is described as occurring from the lateral side of the bulb.…”

Section: Discussionsupporting

confidence: 78%

The vomeronasal system of the newborn capybara: a morphological and immunohistochemical study

Torres

Ortiz‐Leal

Villamayor

et al. 2020

Sci Rep

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the vomeronasal system (VnS) is responsible for the perception mainly of pheromones and kairomones. primarily studied in laboratory rodents, it plays a crucial role in their socio-sexual behaviour. As a wild rodent, the capybara offers a more objective and representative perspective to understand the significance of the system in the Rodentia, avoiding the risk of extrapolating from laboratory rodent strains, exposed to high levels of artificial selection pressure. We have studied the main morphological and immunohistochemical features of the capybara vomeronasal organ (Vno) and accessory olfactory bulb (AoB). the study was done in newborn individuals to investigate the maturity of the system at this early stage. We used techniques such as histological stains, lectinslabelling and immunohistochemical characterization of a range of proteins, including G proteins (Gαi2, Gαo) and olfactory marking protein. As a result, we conclude that the VNS of the capybara at birth is capable of establishing the same function as that of the adult, and that it presents unique features as the high degree of differentiation of the AOB and the active cellular migration in the vomeronasal epithelium. All together makes the capybara a promising model for the study of chemical communication in the first days of life. The vomeronasal system (VNS) is the sensorial system responsible in most vertebrates for the detection of chemosensory signals linked to innate socio-sexual behaviours 1,2. In mammals, the VNS presents a high morphofunctional 3 and genomic 4 diversity among different species. The vomeronasal organ (VNO) specialises in detecting pheromones for the purpose of reproductive behaviours such as maternal aggression and sexual attraction 5. The VNS is also involved in the recognition of major histocompatibility complex (MHC) associated peptides 6 , kairomones 7 and aversive molecules 8. By performing an in-depth study of the macroscopic and microscopic morphological characteristics of the vomeronasal system in the newborn capybara, we aimed to achieve two objectives. On the one hand, we aimed to obtain general information regarding the vomeronasal system in a rodent model that is distinct from most studied laboratory rodents. On the other hand, because the capybara is a precocial animal species, we aimed to determine the degree to which the capybara vomeronasal system morphology at birth has adapted to the requirements of a demanding socio-cognitive environment. Most studies of the VNS have been done on laboratory rodent strains, exposed to artificial selection pressure that do not reflect the selection pressure present in the wild. Therefore, these laboratory strains present significant genetic and behavioural differences compared with wild rodent models 9. The laboratory mouse (Mus musculus) and rat (Rattus norvegicus) may not be representative of all animals that make up this family. A remarkable differential feature among rodents is the altricial character of mice and rats, compared with the precocial character presented by hys...

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Section: Discussionsupporting

confidence: 78%

The vomeronasal system of the newborn capybara: a morphological and immunohistochemical study

Torres

Ortiz‐Leal

Villamayor

et al. 2020

Sci Rep

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“…We have used (1) a lectin that comes from gorse, the Ulex europaeus agglutinin (UEA), α-L-fucose speci c , (2) the α-galactose-speci c BSI-B 4 that comes from Bandeiraea simplicifolia, and (3) Lycopersicon esculentum agglutinin (LEA), coming from tomato with a high a nity for N-acetyl-β-D-glucosamine oligomers ( Table 1). These stains selectively recognise the different components of the olfactory and vomeronasal pathways in some species [52].…”

Section: Histochemical and Immunohistochemical Stainingmentioning

confidence: 99%

“…The histochemical and immunohistochemical protocols followed by the authors have been fully described in previous contributions [51,52]. Brie y:…”

Section: Histochemical and Immunohistochemical Stainingmentioning

confidence: 99%

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Insights Into the Zebrafish Olfactory System: A comprehensive Structural, Lectin- and Immunohistochemical Approach

Villamayor

Arana

Coppel

et al. 2020

Preprint

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Fish chemosensory olfactory receptors allow them to detect a wide range of water-soluble chemicals, that mediate fundamental behaviours. Zebrafish possess a well-developed sense of smell which governs reproduction, appetite, and fear responses. The spatial organization of functional properties within the olfactory epithelium and bulb are comparable to those of mammals, making this species suitable for studies of olfactory differentiation and regeneration and neuronal representation of olfactory information. The advent of genomic techniques has been decisive for the discovery of specific olfactory cell types and the identification of cell populations expressing vomeronasal receptors. These advances have marched ahead of morphological and neurochemical studies. This study aims to fill the existing gap in specific histological, lectin-histochemical and immunohistochemical studies on the olfactory rosette and the olfactory bulb of the zebrafish. Tissue dissection and microdissection techniques were employed, followed by histological staining techniques, lectin-histochemical labelling (UEA, LEA, BSI-B4) and immunohistochemistry using antibodies against G proteins subunits ao and ai2, growth-associated protein-43, calbindin, calretinin, glial-fibrillary-acidic-protein and luteinizing-hormone-releasing-hormone. The results obtained enrich the available information on the neurochemical patterns of the zebrafish olfactory system, pointing to a greater complexity than the one currently considered, especially when taking into account the peculiarities of the nonsensory epithelium.

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“…Accordingly, some behavioural data regarding the rabbit mammary pheromone have been collected (Coureaud et al 2010; Charra et al 2013; Schneider et al 2016), which along with a comprehensive study of the vomeronasal system at anatomical level (Villamayor et al 2018, 2020), highlighted the relevance of chemocommunication in this species. The rabbit VNO was reported to be highly developed with many specific morphological features (Villamayor et al 2018), but surprisingly, it has not been considered in most vomeronasal phylogenetic studies.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive analysis of vomeronasal organ transcriptome reveals variable gene expression depending on age and function in rabbits

Villamayor¹,

Robledo

Fernandez³

et al. 2020

Preprint

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The vomeronasal organ (VNO) is a chemosensory organ specialized in the detection of pheromones and consequently the regulation of behavioural responses mostly related to reproduction. VNO shows a broad variation on its organization, functionality and gene expression in vertebrates, and although the species analyzed to date have shown very specific features, its expression patterns have only been well-characterized in mice. Despite rabbits represent a model of chemocommunication, unfortunately no genomic studies have been performed on VNO of this species to date. The capacity of VNO to detect a great variety of different stimuli suggests a large number of genes with complex organization to support this function. Here we provide the first comprehensive gene expression analysis of the rabbit VNO through RNA-seq across different sexual maturation stages. We characterized the VNO transcriptome, updating the number of the two main vomeronasal receptor (VR) families, 129 V1R and 70 V2R. Among others, the expression of transient receptor potential channel 2 (TRPC2), a crucial cation channel generating electrical responses to sensory stimulation in vomeronasal neurons, along with the specific expression of some fomyl-peptide receptors and H2-Mv genes, both known to have specific roles in the VNO, revealed a the particular gene expression repertoire of this organ, but also its singularity in rabbits. Moreover, juvenile and adult VNO transcriptome showed consistent differences, which may indicate that these receptors are tuned to fulfill specific functions depending on maturation age. We also identified VNO-specific genes, including most VR and TRPC2, thus confirming their functional association with the VNO. Overall, these results represent the genomic baseline for future investigations which seek to understand the genetic basis of behavioural responses canalized through the VNO.HIGHLIGHTSFirst description of the rabbit vomeronasal organ (VNO) transcriptomeVNO contains a unique gene repertoire depending on the speciesHigh fluctuation of the VNO gene expression reveals changes dependent on age and specific functionsMost vomeronasal-receptors (VR) and transient receptor potential channel 2 (TRPC2) genes are VNO-specificReproduction-related genes shows a wide expression pattern

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Structural, morphometric and immunohistochemical study of the rabbit accessory olfactory bulb

Cited by 25 publications

References 107 publications

The vomeronasal system of the newborn capybara: a morphological and immunohistochemical study

The vomeronasal system of the newborn capybara: a morphological and immunohistochemical study

Insights Into the Zebrafish Olfactory System: A comprehensive Structural, Lectin- and Immunohistochemical Approach

A comprehensive analysis of vomeronasal organ transcriptome reveals variable gene expression depending on age and function in rabbits

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