Structural, Morphological and Biological Features of ZnO Nanoparticles Using Hyphaene thebaica (L.) Mart. Fruits

Mohamed, Hamza Elsayed Ahmed; Afridi, Saifullah; Khalil, Ali Talha; Zia, Dania; Shinwari, Zabta Khan; Dhlamini, M.S.; Mâaza, M.

doi:10.1007/s10904-020-01490-0

Cited by 30 publications

(16 citation statements)

References 70 publications

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“…Abbasi et al [70] also reported the moderately toxic nature of green-synthesized ZnONPs (51.7 nm) against brine shrimp. The dose-dependent cytotoxicity of ZnONPs synthesized from Hyphaene thebaica towards brine shrimp was described by Mohamed et al [91]. Dobretsov et al [92] showed that the toxicity of ZnONPs towards Artemia salina depends on the morphology and concentration of ZnONPs, where ZnO nano-rods (width 80 nm) were least toxic.…”

Section: Caspase-3 Gene Expression and Caspase-3/7 Activitymentioning

confidence: 90%

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

et al. 2021

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The green synthesis of nanoparticles has emerged as a simple, safe, sustainable, reliable and eco-friendly protocol. Among different types of NPs, green-synthesized zinc oxide NPs (ZnONPs) show various promising biological uses due to their interesting magnetic, electrical, optical and chemical characteristics. Keeping in view the dependence of the therapeutic efficacy of NPs on their physico-chemical characteristics, the green synthesis of ZnONPs using Casuarina equisetifolia leaf extract under UV-A and UV-C light was carried out in this study. UV-irradiation helped to control the size and morphology of ZnONPs by exciting the electrons in the photoactive compounds of plant extracts to enhance the bio-reduction of ZnO into ZnONPs. C. equisetifolia leaf extract was found enriched with phenolic (2.47 ± 0.12 mg GAE/g DW) and flavonoid content (0.88 ± 0.28 mg QE/g DW) contributing to its 74.33% free-radical scavenging activity. FTIR spectra showed the involvement of polyphenols in the bio-reduction, stabilization and capping of ZnONPs. Moreover, SEM-EDX and XRD analyses showed great potential of UV-C light in yielding smaller (34–39 nm) oval-shaped ZnONPs, whereas UV-A irradiation resulted in the formation of fairly spherical 67–71 nm ZnONPs and control ZnONPs were of mixed shape and even larger size (84–89 nm). Green-synthesized ZnONPs, notably CE-UV-C-ZnONPs, showed promising anti-bacterial activities against Bacillus subtilis, Pseudomonas fluorescens and Pseudomonas aeruginosa. Moreover, ZnONPs also enhanced ROS production which led to a significant loss of mitochondrial membrane potential and activated caspase-3 gene expression and caspase-3/7 activity in human hepatocellular carcinoma (HepG2) cells. CE-UV-C-ZnONP treatment reduced HepG2 cell viability to as low as 36.97% owing to their unique shape and smaller size. Lastly, ZnONPs were found to be highly biocompatible towards brine shrimp and human red blood cells suggesting their bio-safe nature. This research study sheds light on the plausible role of UV radiation in the green synthesis of ZnONPs with reasonable control over their size and morphology, thus improving their biological efficacy.

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Section: Caspase-3 Gene Expression and Caspase-3/7 Activitymentioning

confidence: 90%

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

et al. 2021

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“…Our results are in accordance with the literature available on ZnO-NP kinase inhibition enzymes. 55 Overall results concluded that the biogenic NPs acquire vital capping and stabilizing agents from plant extracts responsible for anticancerous capability. Biogenic ZnO-NPs were observed to inhibit Streptomyces strain in a dose-dependent manner, as shown in Figure 4 A.…”

Section: Biological Applicationsmentioning

confidence: 92%

Green Synthesis of Zinc Oxide (ZnO) Nanoparticles Using Aqueous Fruit Extracts of Myristica fragrans: Their Characterizations and Biological and Environmental Applications

et al. 2021

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In the present work, bioaugmented zinc oxide nanoparticles (ZnO-NPs) were prepared from aqueous fruit extracts of Myristica fragrans . The ZnO-NPs were characterized by different techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and thermogravimetric analysis (TGA). The crystallites exhibited a mean size of 41.23 nm measured via XRD and were highly pure, while SEM and TEM analyses of synthesized NPs confirmed their spherical or elliptical shape. The functional groups responsible for stabilizing and capping of ZnO-NPs were confirmed using FTIR analysis. The ζ-size and ζ-potential of synthesized ZnO-NPs were reported as 66 nm and −22.1 mV, respectively, via the DLS technique can be considered as moderate stable colloidal solution. Synthesized NPs were used to evaluate for their possible antibacterial, antidiabetic, antioxidant, antiparasitic, and larvicidal properties. The NPs were found to be highly active against bacterial strains both coated with antibiotics and alone. Klebsiella pneumoniae was found to be the most sensitive strain against NPs (27 ± 1.73) and against NPs coated with imipinem (26 ± 1.5). ZnO-NPs displayed outstanding inhibitory potential against enzymes protein kinase (12.23 ± 0.42), α-amylase (73.23 ± 0.42), and α-glucosidase (65.21 ± 0.49). Overall, the synthesized NPs have shown significant larvicidal activity (77.3 ± 1.8) against Aedes aegypti , the mosquitoes involved in the transmission of dengue fever. Similarly, tremendous leishmanicidal activity was also observed against both the promastigote (71.50 ± 0.70) and amastigote (61.41 ± 0.71) forms of the parasite. The biosynthesized NPs were found to be excellent antioxidant and biocompatible nanomaterials. Biosynthesized ZnO-NPs were also used as photocatalytic agents, resulting in 88% degradation of methylene blue dye in 140 min. Owing to their eco-friendly synthesis, nontoxicity, and biocompatible nature, ZnO-NPs synthesized from M. fragrans can be exploited as potential candidates for biomedical and environmental applications.

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“…The word "nano" is taken from Greek word which means "extremely small or dwarf." Nanotechnology is a rapidly growing field and has found its way in different commercial areas including cosmetics, food, medicine, health care, drug delivery, gene delivery, agriculture, catalysis, energy science, and environment (Khalil, Khan, et al, 2021;Khalil, Ovais, Ullah, Ali, Jan, et al, 2020;Mohamed et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Rhamnella gilgitica functionalized green synthesis of ZnONPs and their multiple therapeutic properties

Abbasi

Iqbal

Israr

et al. 2022

Microscopy Res & Technique

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In the recent years, green synthesis of zinc oxide nanoparticles (ZnONPs) using plant extracts and phytochemicals has gained significant attention. In present research study, facile, green, and tunable ZnONPs were biosynthesized from Rhamnella gilgitica leaf aqueous extract as a strong reducing and stabilizing agents. The prepared ZnONPs@Rhamnella were characterized and validated using common nanotechnology techniques (UV-Vis, XRD, EDX, FT-IR, SEM, TEM, DLS, and Raman) and revealed spherical morphology with particle size ~21 nm. The asynthesized ZnONPs were further evaluated for different biological applications. Strong antimicrobial efficacies were reported for ZnONPs using discdiffusion method and were capable of rendering significant antimicrobial potential.ZnONPs were evaluated against HepG2 (IC 50 : 18.40 μg/ml) and HUH7 (IC 50 : 20.59 μg/ml) cancer cell lines and revealed strong anticancer properties. Dose-dependent MTT cytotoxicity assay was confirmed using Leishmania tropica "KWH23 strain" (promastigote: IC 50 : 26.78 μg/ml and amastigote: IC 50 : 29.57 μg/ml). Antioxidant activities (DPPH: 93.36%, TAC: 72.43%) were performed to evaluate their antioxidant potentials. Further, protein kinase and α-amylase inhibition assays were determined. Biocompatibility assays were done using human RBCs and macrophages thus revealed biosafe and non-toxic nature of ZnONPs@Rhamnella. In current experiment, we concluded that greenly orchestrated ZnONPs is an attractive, non-toxic and ecofriendly candidate and showed potential biological activities. In future, different clinical trials and in vivo studies are necessary for the confirmation of these remedial properties of ZnONPs using different animal models. Research highlights1. Greenly orchestrated ZnONPs were synthesized using Rhamnella gilgitica leaves broth.2. Analytical techniques such as UV, SEM, TEM, XRD, FTIR, DLS, and Raman confirmed synthesis of ZnONPs.3. Green ZnONPs determined strong antimicrobial, cytotoxic, and antioxidant potentials. 4. Significant enzyme inhibition and biocompatibility assays were investigated.

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Structural, Morphological and Biological Features of ZnO Nanoparticles Using Hyphaene thebaica (L.) Mart. Fruits

Cited by 30 publications

References 70 publications

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

Green Synthesis of Zinc Oxide (ZnO) Nanoparticles Using Aqueous Fruit Extracts of Myristica fragrans: Their Characterizations and Biological and Environmental Applications

Rhamnella gilgitica functionalized green synthesis of ZnONPs and their multiple therapeutic properties

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