Structural modifications in the mesophyll associated with cryopreservation of seven Hypericum species

Stoyanova-Koleva, D.; Stefanova, M.; Ganeva, Tsveta; Čellárová, Eva

doi:10.1007/s10535-015-0528-8

Cited by 7 publications

(7 citation statements)

References 12 publications

(6 reference statements)

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“…Previous studies have found that H. perforatum leaf morphology ( e.g. leaf length/width, stem height and DG) are significantly affected by species, geographic, light and temperature conditions ( Briskin and Gawienowski, 2001 ; Walker et al., 2001 ; Cirak et al., 2007 ; Stoyanova-Koleva et al., 2015 ; Su et al., 2021 ). Since Hyp biosynthesis occurs in dark glands (DG) and secretory cells (SC) is associated with Hyp accumulation ( Lv and Hu, 2001 ; Zobayed et al., 2006 ; Kornfeld et al., 2007 ; Rizzo et al., 2019 ), these organelles were monitored.…”

Section: Resultsmentioning

confidence: 99%

Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum

Jin²,

Li³

et al. 2022

Front. Plant Sci.

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Hypericum perforatum, commonly known as St John’s wort, is a perennial herb that produces the anti-depression compounds hypericin (Hyp) and hyperforin. While cool temperatures increase plant growth, Hyp accumulation as well as changes transcript profiles, alterations in leaf structure and genes expression specifically related to Hyp biosynthesis are still unresolved. Here, leaf micro- and ultra-structure is examined, and candidate genes encoding for photosynthesis, energy metabolism and Hyp biosynthesis are reported based on transcriptomic data collected from H. perforatum seedlings grown at 15 and 22°C. Plants grown at a cooler temperature exhibited changes in macro- and micro-leaf anatomy including thicker leaves, an increased number of secretory cell, chloroplasts, mitochondria, starch grains, thylakoid grana, osmiophilic granules and hemispherical droplets. Moreover, genes encoding for photosynthesis (64-genes) and energy (35-genes) as well as Hyp biosynthesis (29-genes) were differentially regulated with an altered growing temperature. The anatomical changes and genes expression are consistent with the plant’s ability to accumulate enhanced Hyp levels at low temperatures.

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Section: Resultsmentioning

confidence: 99%

Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum

Jin²,

Li³

et al. 2022

Front. Plant Sci.

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“…Another way in which the cryoprotectant agents relate to restoration of the viability after cryostorage is preservation of the photosynthetic apparatus. The protective roles of ABA and mannitol were confirmed on cellular and sub-cellular levels by transmission electron microscopy (TEM) in H. perforatum and H. rumeliacum plants ( Stoyanova-Koleva et al, 2013 , 2015 ). The effect of ABA depended on the species, duration of the treatment and cooling regime; in H. perforatum , although no deleterious post-cryogenic alterations of the internal membrane system under 10-day pre-treatment with ABA followed by slow-cooling were seen, the destruction of the chloroplast membranes was observed after vitrification ( Stoyanova-Koleva et al, 2015 ).…”

Section: Crucial Processes For Successful Cryopreservation In mentioning

confidence: 96%

“…The protective roles of ABA and mannitol were confirmed on cellular and sub-cellular levels by transmission electron microscopy (TEM) in H. perforatum and H. rumeliacum plants ( Stoyanova-Koleva et al, 2013 , 2015 ). The effect of ABA depended on the species, duration of the treatment and cooling regime; in H. perforatum , although no deleterious post-cryogenic alterations of the internal membrane system under 10-day pre-treatment with ABA followed by slow-cooling were seen, the destruction of the chloroplast membranes was observed after vitrification ( Stoyanova-Koleva et al, 2015 ). In H. rumeliacum shoot tips cryopreserved by vitrification, an optimal efficacy of the ABA-treatment was already observed after 3-day pre-culture ( Stoyanova-Koleva et al, 2013 ).…”

Section: Crucial Processes For Successful Cryopreservation In mentioning

confidence: 96%

“…To obtain additional information on the FT in plants, the extent of freezing injury in terms of the integrity of photosynthetic apparatus has been intensively investigated at both, functional and structural levels ( Rumich-Bayer and Krause, 1986 ; He et al, 2002 ; Su et al, 2015 ). Based on histological and TEM examination of the effect of freezing temperature on leaf tissue organization and chloroplast ultrastructure of seven Hypericum species differing in the LT 50 values ( Petijová et al, 2014 ; Stoyanova-Koleva et al, 2015 ), no plausible connection between the predicted FT and response to cryoinjury was observed. Under the experimental conditions including a 10-day ABA preculture, cryoprotection with PVS3 and rapid cooling by direct immersion into LN, the well-developed leaves with regularly structured mesophyll in post-cryogenic regenerants of H. annulatum, H. tomentosum, H. rumeliacum, H. humifusum , and H. kalmianum possessing a various extent of the FT were seen.…”

Section: Post-cryogenic Recovery In Relation To Freezing Tolerance Inmentioning

confidence: 99%

“…A partial damage of chloroplasts ultrastructure was only observed in the freezing-tolerant H. perforatum. However, a remarkably increased thickness of the leaf assimilation parenchyma indicates a possible compensatory mechanism to overcome the low-temperature stress in that species ( Stoyanova-Koleva et al, 2015 ).…”

Section: Post-cryogenic Recovery In Relation To Freezing Tolerance Inmentioning

confidence: 99%

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Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

Bruňáková

Čellárová

2016

Front. Plant Sci.

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In the genus Hypericum, cryoconservation offers a strategy for maintenance of remarkable biodiversity, emerging from large inter- and intra-specific variability in morphological and phytochemical characteristics. Long-term cryostorage thus represents a proper tool for preservation of genetic resources of endangered and threatened Hypericum species or new somaclonal variants with unique properties. Many representatives of the genus are known as producers of pharmacologically important polyketides, namely naphthodianthrones and phloroglucinols. As a part of numerous in vitro collections, the nearly cosmopolitan Hypericum perforatum – Saint John’s wort – has become a suitable model system for application of biotechnological approaches providing an attractive alternative to the traditional methods for secondary metabolite production. The necessary requirements for efficient cryopreservation include a high survival rate along with an unchanged biochemical profile of plants regenerated from cryopreserved cells. Understanding of the processes which are critical for recovery of H. perforatum cells after the cryogenic treatment enables establishment of cryopreservation protocols applicable to a broad number of Hypericum species. Among them, several endemic taxa attract a particular attention due to their unique characteristics or yet unrevealed spectrum of bioactive compounds. In this review, recent advances in the conventional two-step and vitrification-based cryopreservation techniques are presented in relation to the recovery rate and biosynthetic capacity of Hypericum spp. The pre-cryogenic treatments which were identified to be crucial for successful post-cryogenic recovery are discussed. Being a part of genetic predisposition, the freezing tolerance as a necessary precondition for successful post-cryogenic recovery is pointed out. Additionally, a beneficial influence of cold stress on modulating naphthodianthrone biosynthesis is outlined.

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Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

2017

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Cryopreservation is widely applied to many economically important species excluding chimera plants which are problematic for long-term conservation. Their storage problems can be circumvented only by cryopreserving meristems. This study looked at the morphogenetic response of shoot tips of periclinal chimera chrysanthemum 'Lady Orange' and 'Lady Salmon', as well as the solid mutant 'Richmond', that were cryopreserved by encapsulation-dehydration technique. By applying 10 µM ABA in the preculture medium followed by 4-day-long dehydration treatment, the explant survival reached up to 67%. Besides the stimulation of typical single shoot recovery, cryopreservation led to direct or indirect multiple shoot formation, shoot malformation, as well as inhibited their spontaneous rooting. Microscopic analysis revealed three types of structural damages of shoot tips which can correspond with their morphogenetic response in recovery culture. No influence of cryostorage on the acclimatisation efficiency of the recovered chrysanthemums was observed.

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Structural modifications in the mesophyll associated with cryopreservation of seven Hypericum species

Cited by 7 publications

References 12 publications

Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum

Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum

Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

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