Structural mechanism of plant aquaporin gating

Törnroth-Horsefield, Susanna; Wang, Yi; Hedfalk, Kristina; Johanson, Urban; Karlsson, Maria; Tajkhorshid, Emad; Neutze, Richard; Kjellbom, Per

doi:10.1038/nature04316

Cited by 754 publications

(850 citation statements)

References 46 publications

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“…Most residues that are in direct contact with the pores, especially those of the ar/R constriction region, are strongly conserved among PIP-type aquaporins, including the crystallized plasma membrane aquaporin SoPIP2;1 from spinach [13]. All 13 PIP1-and PIP2-type homologs from Arabidopsis contain an identical ar/R filter consisting of phenylalanine (Phe), histidine (His), threonine (Thr) and arginine (Arg).…”

Section: Simulation Of Small Solute Conduction In a Plant Aquaporinmentioning

confidence: 99%

Molecular determinants of ammonia and urea conductance in plant aquaporin homologs

et al. 2008

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Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2;1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH 3 , and a residue without direct contact to the pore lumen was critical for conduction in the mutants.

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Section: Simulation Of Small Solute Conduction In a Plant Aquaporinmentioning

confidence: 99%

Molecular determinants of ammonia and urea conductance in plant aquaporin homologs

et al. 2008

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“…A number of studies have also shown that AQPs play a role in various disorders from renal disease to cerebral oedema (Benarroch, 2007, Schrier andCadnapaphornchai, 2003) and even cancer (Yin et al, 2008, Chae et al, 2008. Consequently AQPs have been highlighted as key drug targets (Huber et al, 2012, Wang et al, 2006 although drugs that target AQPs for clinical use remain to be discovered. This diverse tissue distribution and involvement in many physiological and pathological processes, is summarised in Table 1.…”

Section: Introductionmentioning

confidence: 99%

An emerging consensus on aquaporin translocation as a regulatory mechanism

Conner

Bill

Conner

2012

Molecular Membrane Biology

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Water can pass through the cell membrane relatively slowly by diffusion. However, in order to maintain water homeostasis, the rapid and specific regulation of cellular water flow is mediated by the aquaporin (AQP) family of membrane protein water channels. Thirteen human AQPs have been identified to date and the majority are highly specific for water while others show selectivity for water, glycerol and other small solutes. The wide range of tissues that are known to express AQPs is reflected by their involvement in many physiological processes and diseases. Receptor mediated translocation, via hormone activation, is an established method of AQP regulation, especially for AQP2. There is now an emerging consensus that the rapid and reversible translocation of other AQPs from intracellular vesicles to the plasma membrane, triggered by a range of stimuli, can confer increased membrane permeability. This review examines new advances that have identified molecular mechanisms of AQP regulation; these include the role of cytoskeletal proteins, kinases, calcium and retention or localisation signals as well as specific triggers of AQP translocation. This article reviews current knowledge of AQP regulation with a focus on rapid and dynamic regulation of sub-cellular AQP translocation in response to a specific trigger.3

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“…Phosphorylation of certain serine residues activates some PIPs, and dephosphorylation rapidly closes them [26]. This aquaporin inactivation can prevent water loss from cells under drought or strong salt stress [16].…”

Section: Water Transport Of Barley Pipsmentioning

confidence: 99%

Barley plasma membrane intrinsic proteins (PIP Aquaporins) as water and CO2 transporters

Katsuhara

Hanba

2008

Pflugers Arch - Eur J Physiol

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We identified barley aquaporins, and demonstrated that one, HvPIP2;1, transports water and CO 2 . Regarding water homeostasis in plants, regulations of aquaporin expression were observed in many plants under several environmental stresses. Under salt stress, a number of plasma-membrane type aquaporins were down-regulated, which can prevent continuous dehydration resulting in cell death. The leaves of transgenic rice plants that expressed the largest amount of HvPIP2;1 showed a 40% increase in internal CO 2 conductance compared with leaves of wild-type rice plants. The rate of CO 2 assimilation also increased in the transgenic plants. The goal of our plant aquaporin research is to determine the key aquaporin species responsible for water and CO 2 transport, and to improve plant water relations, stress tolerance, CO 2 uptake/assimilation, and plant productivity via molecular breeding of aquaporins.

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Structural mechanism of plant aquaporin gating

Cited by 754 publications

References 46 publications

Molecular determinants of ammonia and urea conductance in plant aquaporin homologs

Molecular determinants of ammonia and urea conductance in plant aquaporin homologs

An emerging consensus on aquaporin translocation as a regulatory mechanism

Barley plasma membrane intrinsic proteins (PIP Aquaporins) as water and CO2 transporters

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