Structural Maintenance of Chromosome Complex in Bacteria

Borgmann, Luise A K Kleine; Graumann, Peter L.

doi:10.1159/000368931

Cited by 16 publications

(10 citation statements)

References 87 publications

(132 reference statements)

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“…RecN and RecO form visible subcellular assemblies on the chromosome 15 to 30 minutes after induction of DNA double strand breaks (DSBs). A single assembly can be seen in a maximum of 70% of cells, no matter how many breaks arose due to added chemicals 17 . Major questions in this process are how RecN and RecO move within cells, at how many sites they assemble, and how long-lived subcellular assemblies are.…”

Section: Resultsmentioning

confidence: 99%

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Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres

Rösch

Altenburger

Oviedo-Bocanegra

et al. 2018

Sci Rep

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Single-particle (molecule) tracking (SPT/SMT) is a powerful method to study dynamic processes in living bacterial cells at high spatial and temporal resolution. We have performed single-molecule imaging of early DNA double-strand break (DSB) repair events during homologous recombination in the model bacterium Bacillus subtilis. Our findings reveal that DNA repair centres arise at all sites on the chromosome and that RecN, RecO and RecJ perform fast, enzyme-like functions during detection and procession of DNA double strand breaks, respectively. Interestingly, RecN changes its diffusion behavior upon induction of DNA damage, from a largely diffusive to a DNA-scanning mode, which increases efficiency of finding all sites of DNA breaks within a frame of few seconds. RecJ continues being bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction. RecO shows a similar change in its mobility as RecN, and also remains bound to sites of damage for few hundred milliseconds. Like RecN, it enters the nucleoid in damaged cells. Our data show that presynaptic preparation of DSBs including loading of RecA onto ssDNA is highly rapid and dynamic, and occurs throughout the chromosome, and not only at replication forks or only at distinct sites where many breaks are processes in analogy to eukaryotic DNA repair centres.

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Section: Resultsmentioning

confidence: 99%

“…1 ). In bacteria, the initial protein to visually assemble on the nucleoid in response to the induction of DSBs is the structural maintenance of chromosomes (SMC)-like protein RecN 16 , 17 , which binds to DNA ends in vitro 18 (Fig. 1 ).…”

Section: Introductionmentioning

confidence: 99%

Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres

Rösch

Altenburger

Oviedo-Bocanegra

et al. 2018

Sci Rep

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“…The chromosome is also actively shaped by the Structural Maintenance of Chromosome (SMC) complex, which forms a structure that encircles DNA (Figure 2Aiii) (Gruber, 2014; Kleine Borgmann and Graumann, 2014; Nolivos and Sherratt, 2014). Typically, the SMC complex consists of the ATPase SMC and the auxiliary proteins ScpA and ScpB (Mascarenhas et al, 2002; Soppa et al, 2002).…”

Section: Packing and Decoding The Genomementioning

confidence: 99%

Subcellular Organization: A Critical Feature of Bacterial Cell Replication

Surovtsev

Jacobs‐Wagner

2018

Cell

160

159

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Spatial organization is a hallmark of all living systems. Even bacteria, the smallest forms of cellular life, display defined shapes and complex internal organization, showcasing a highly structured genome, cytoskeletal filaments, localized scaffolding structures, dynamic spatial patterns, active transport, and occasionally, intracellular organelles. Spatial order is required for faithful and efficient cellular replication and offers a powerful means for the development of unique biological properties. Here, we discuss organizational features of bacterial cells and highlight how bacteria have evolved diverse spatial mechanisms to overcome challenges cells face as self-replicating entities.

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“…Higher levels of Smc in B. subtilis cells result in chromosome hyper-compaction [ 7 ]. We wished to investigate if Smc and/or its complex partners ScpA and ScpB, which do not bind to DNA by themselves [ 10 ], can also condense chromosomes when expressed in other bacterial species. For this purpose, we chose E. coli cells as a model system, and employed a two-plasmids expression system.…”

Section: Objectivementioning

confidence: 99%

Bacillus subtilis Smc condenses chromosomes in a heterologous cell system, which is down-regulated by ScpAB

Knust

Graumann

2020

BMC Res Notes

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Objective Structural maintenance of chromosomes (SMC) proteins are key players in chromosome dynamics in all types of organisms. The so-called condensin subfamily is essential for chromosome condensation in eukaryotic cells, as is the bacterial SMC complex (called MukBEF in Escherichia coli). We expressed the Bacillus subtilis Smc protein and its two complex partners ScpA and ScpB in E. coli cells, and monitored effects on chromosome compaction by DNA staining of live cells using epifluorescence microscopy. Data description We show that expression of BsSmc leads to strong chromosome compaction, while expression of ScpAB does not show any effect. Chromosome compaction by Smc was also found for mutant versions lacking ATP binding or ability for head engagement, and was counteracted by concomitant expression of ScpAB. Our findings show that the SMC complex can act as autonomous condensation system in a heterologous bacterial host system, for which neither ATP binding nor ATP hydrolysis are required. Our investigation suggests that the negative effect on compaction activity of Smc exerted by ScpAB in vivo does not involve an effect on ATPase activity, but more likely a stabilization of the engagement of head domains, which in turn may affect ATPase activity.

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Structural Maintenance of Chromosome Complex in Bacteria

Cited by 16 publications

References 87 publications

Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres

Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres

Subcellular Organization: A Critical Feature of Bacterial Cell Replication

Bacillus subtilis Smc condenses chromosomes in a heterologous cell system, which is down-regulated by ScpAB

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