Structural investigations on native collagen type I fibrils using AFM

Strasser, Stefan; Zink, Albert; Janko, Marek; Heckl, Wolfgang M.; Thalhammer, Stefan

doi:10.1016/j.bbrc.2006.12.114

Cited by 95 publications

(70 citation statements)

References 28 publications

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“…The characteristic of D-banding was determined by measuring the length of the gap and overlap regions of collagen fibrils [15]. The surfaces were first imaged at a 10 ×10 µm scan area to identify the collagen fibril pattern followed by rescanning at a higher resolution with a scan area of 5 × 5 µm.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that the mechanical behavior of tissue is dependent on the collagen fibrils [11,12,13,14,15]. Information about the mechanical properties of collagen is not only essential to explain the macroscopic biophysics of different tissues but can also contribute to the understanding of the microscopic structure of collagen fibrils themselves.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, numerous studies on the structural and mechanical properties of biological samples at an ultrastructural level have been successfully conducted using second-harmonic generation multiphoton imaging [19,20] and atomic force microscopy (AFM) [15,21,22,23,24,25,26,27,28,29,30]. Second-harmonic generation multiphoton microscopy is an imaging technique that is capable of studying living tissue in vivo, such as skin tissue analysis, without the need of staining and fixation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characteristics and Young's Modulus of Collagen Fibrils from Expanded Skin Using Anisotropic Controlled Rate Self-Inflating Tissue Expander

Manssor

Radzi

Yahya

et al. 2016

Skin Pharmacol Physiol

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Mechanical properties of expanded skin tissue are different from normal skin, which is dependent mainly on the structural and functional integrity of dermal collagen fibrils. In the present study, mechanical properties and surface topography of both expanded and nonexpanded skin collagen fibrils were evaluated. Anisotropic controlled rate self-inflating tissue expanders were placed beneath the skin of sheep's forelimbs. The tissue expanders gradually increased in height and reached equilibrium in 2 weeks. They were left in situ for another 2 weeks before explantation. Expanded and normal skin samples were surgically harvested from the sheep (n = 5). Young's modulus and surface topography of collagen fibrils were measured using an atomic force microscope. A surface topographic scan showed organized hierarchical structural levels: collagen molecules, fibrils and fibers. No significant difference was detected for the D-banding pattern: 63.5 ± 2.6 nm (normal skin) and 63.7 ± 2.7 nm (expanded skin). Fibrils from expanded tissues consisted of loosely packed collagen fibrils and the width of the fibrils was significantly narrower compared to those from normal skin: 153.9 ± 25.3 and 106.7 ± 28.5 nm, respectively. Young's modulus of the collagen fibrils in the expanded and normal skin was not statistically significant: 46.5 ± 19.4 and 35.2 ± 27.0 MPa, respectively. In conclusion, the anisotropic controlled rate self-inflating tissue expander produced a loosely packed collagen network and the fibrils exhibited similar D-banding characteristics as the control group in a sheep model. However, the fibrils from the expanded skin were significantly narrower. The stiffness of the fibrils from the expanded skin was higher but it was not statistically different.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characteristics and Young's Modulus of Collagen Fibrils from Expanded Skin Using Anisotropic Controlled Rate Self-Inflating Tissue Expander

Manssor

Radzi

Yahya

et al. 2016

Skin Pharmacol Physiol

View full text Add to dashboard Cite

show abstract

“…At the fibrillar level, direct mechanical measurements have only recently become possible by atomic force microscopy (AFM) and microelectromechanical systems (MEMS). The mechanical properties of single collagen fibrils have been measured using AFM-based tensile [7][8][9][10], nanoindentation [11][12][13][14][15][16][17][18][19], and bending [20][21][22] tests, and MEMS-based tensile [23][24][25] tests.…”

Section: Introductionmentioning

confidence: 99%

Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Yamamoto¹

2017

EJB

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Abstract:Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 µm, respectively.

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“…To reproduce the exceptional strength and toughness seen in soft load-bearing tissues, one strategy is to combine an energy-dissipative tough hydrogel with rigid yet flexible fibers to create a composite, similar to fibrous tissues. [21][22][23] In this composite concept, the rigid fiberbased component increases the specific strength while the gel matrix dissipates energy. Based on this concept, some attempts have been made to fabricate fiber reinforced hydrogel composites.…”

mentioning

confidence: 99%

Energy‐Dissipative Matrices Enable Synergistic Toughening in Fiber Reinforced Soft Composites

Huang

King

Sun

et al. 2017

Adv Funct Materials

133

131

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(1 of 10) 1605350 materials exhibiting both excellent loadbearing capacity and fracture resistance, as can be observed from both hard tissues [3][4][5][6][7] (e.g., nacre and bone) and soft tissues (e.g., ligament and tendon), [8][9][10] by combining rigid, brittle components (either inorganic or organic) and soft, organic components into composite materials. Most of these natural materials have highly complex hierarchical architectures existing over multiple length scales, which results in composite properties that far exceed what could be expected from a simple combination of the individual components. [3] Many researchers have attempted to mimic the unique natural structures of tough, hard hybrid materials, but only a few studies have achieved remarkable success comparable to that of nature. [11][12][13][14] For example, a bioinspired alumina hybrid material with specific strength and toughness comparable to aluminum alloys was synthesized by combining a hard yet brittle ceramic with relatively soft poly(methyl methacrylate), where nacre-like multiple toughening mechanisms at multiple scales resulted in exceptional fracture resistance. [12] As a vital class of soft materials, tough hydrogels have shown strong potential as structural biomaterials. [15][16][17][18][19][20] These hydrogels alone, however, still possess limited mechanical properties (low modulus) when compared to some load-bearing tissues, e.g., ligaments and tendons. To reproduce the exceptional strength and toughness seen in soft load-bearing tissues, one strategy is to combine an energy-dissipative tough hydrogel with rigid yet flexible fibers to create a composite, similar to fibrous tissues. [21][22][23] In this composite concept, the rigid fiberbased component increases the specific strength while the gel matrix dissipates energy. Based on this concept, some attempts have been made to fabricate fiber reinforced hydrogel composites. [24][25][26][27][28][29] Utilizing this technique, researchers have been able to increase and tune the stiffness and toughness achievable with hydrogel-based systems. However, developing soft composites with synergistically improved mechanical properties, such as those seen in hard bioinspired composites, is still a challenge.Recently, our group has developed a new class of tough hydrogels, polyampholyte (PA) gels (fracture energy, T = 3000 J m −2 ), based on multiple ionic bonds acting as reversible sacrificial bonds in the gel network. [18,30] Interestingly, PA gels also demonstrate unique interfacial bonding to charged surfaces, either positive or negative, due to the self-adjustable Coulombic interaction of the dynamic ionic bonds of the PA. [31] The PA gels are synthesized from radical polymerization of oppositely Energy-Dissipative Matrices Enable Synergistic Toughening in Fiber Reinforced Soft CompositesYiwan Huang, Daniel R. King, Tao Lin Sun, Takayuki Nonoyama, Takayuki Kurokawa, Tasuku Nakajima, and Jian Ping Gong* Tough hydrogels have shown strong potential as structural biomaterials. These hydrogels a...

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Structural investigations on native collagen type I fibrils using AFM

Cited by 95 publications

References 28 publications

Characteristics and Young's Modulus of Collagen Fibrils from Expanded Skin Using Anisotropic Controlled Rate Self-Inflating Tissue Expander

Characteristics and Young's Modulus of Collagen Fibrils from Expanded Skin Using Anisotropic Controlled Rate Self-Inflating Tissue Expander

Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Energy‐Dissipative Matrices Enable Synergistic Toughening in Fiber Reinforced Soft Composites

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