Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme

Li, Bo; Xinjun, Wei; Sun, Junliang; Xu, Shengyu

doi:10.1016/j.carres.2006.03.035

Cited by 139 publications

(63 citation statements)

References 18 publications

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“…Another study demonstrated the immunostimulatory and anti-tumor effect of ginsan [24]. Fucoidan, a sulfated polysaccharide, is one of the main components of the cell-wall matrix of brown seaweeds; fucoidans from various brown algae have antitumor activity [1,25]. These reports suggest that immunostimulatory polysaccharides isolated from various natural sources are good candidates for development of antitumor agents.…”

Section: Effects Of Hfae On the Production Of No And Cytokines In Rawmentioning

confidence: 99%

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Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Yoon

Lee²,

Park³

et al. 2011

Biotechnol Bioproc E

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Hizikia fusiforme is a commonly used food that possesses potent anti-bacterial, anti-fungal, and antiinflammatory activities. The immunostimulatory activities of aqueous extract of Hizikia fusiforme (HFAE) in RAW 264.7 macrophages and whole spleen cells were investigated. HFAE activated RAW 264.7 macrophages to produce cytokines such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in a dose-dependent manner. In addition, HFAE induced the mRNA expression of TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophages. Moreover, HFAE stimulated proliferation of whole spleen cells and reference mitogen. Taken together, the results demonstrate that HFAE potently activates the immune function by regulating NO, TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophage and promoting spleen cell proliferation.

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Section: Effects Of Hfae On the Production Of No And Cytokines In Rawmentioning

confidence: 99%

“…Hizikia fusiforme is a brown seaweed that mainly grows in the temperate seaside areas of the northwest Pacific, including China, Japan [1], and Korea, and which has been commonly used as a health food in Korea. H. fusiforme contain fucoidans, polysaccharides that contain a number of fucose and sulfate ester groups.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Yoon

Lee²,

Park³

et al. 2011

Biotechnol Bioproc E

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“…In this study, [16] Ethyl alcohol extract of H. fusiforme was prepared as previously described [12]. Briefly, dried H. fusiforme were extracted in 80% ethyl alcohol at a ratio of 10 ml/g for 24 hr in a refrigerator at 4°C.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cell Invasion by Ethyl Alcohol Extracts of Hizikia fusiforme in AGS Human Gastric Adenocarcinoma Cells

Choi

2010

Journal of Life Science

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In this study, we investigated the effects of ethyl alcohol extracts of Hizikia fusiforme (EHF) on the correlation between tightening of tight junctions (TJs) and anti-invasive activity in human gastric adenocarcinoma AGS cells. Inhibitory effects of EHF on cell proliferation, motility, and invasiveness were found to be associated with increased tightness of the TJs, which was demonstrated by an increase in transepithelial electrical resistance. Activities of matrix metalloprotease (MMP)-2 and -9 in AGS cells were dose-dependently inhibited by treatment with EHF, and this was also correlated with a decrease in expression of their mRNA and proteins; however, tissue inhibitor of metalloproteinase (TIMP)-1 and -2 mRNA levels were increased. Additionally, immunoblotting results indicated that EHF repressed the levels of claudin proteins (claudin-1, -3, and -4), major components of TJs that play key roles in control and selectivity of paracellular transport. Furthermore, EHF decreased expression of insulin such as growth factor-1 receptor proteins, while concurrently increasing that of thrombospondin-1 and E-cadherin. In conclusion, these results suggest that EHF treatment may inhibit tumor cell motility and invasion, and therefore act as a dietary source to decrease the risk of cancer metastasis.

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“…Various reports have already addressed the preliminary characterization (Nishide et al, 1987;Nishino and Nagumo, 1987;Shiroma et al, 2003) and anticoagulant activity (Dobashi et al, 1989) of fucoidans from H. fusiformis, as well as the structure of a H. fusiformis fucoidan containing a fucose-free core (Li et al, 2006). However, the structure of fucoidan containing high-fucose and ester sulfate has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Structural Study of Fucoidan from the Brown Seaweed Hizikia fusiformis

Shiroma

Konishi

Uechi

et al. 2008

FSTR

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We investigated the structure of fucoidan from Hizikia fusiformis. Using DEAE-Sepharose column chromatography, fucoidan was fractionated into four groups. F4, main fraction of fucoidan, contained 38 % (w/w) of ester sulfate and had the simplest sugar component (l-Fuc: d-Gal=7:3). The structure of F4 was discussed using de-sulfation, 2D-NMR and methylation analysis and proposed as % in molar ratio of the hydroxyl group is sulfated.Keywords: fucoidan, Hizikia fusiformis, brown seaweed, de-sulfation, 2D-NMR, methylation *To whom correspondence should be addressed. E-mail: tako@agr.u-ryukyu.ac.jp IntroductionFucoidan, a cell-wall matrix polysaccharide in brown seaweed and some echinoderms, is composed of α-l-fucose and ester sulfate, including minor amounts of d-Gal, d-Man, d-Xyl, d-Glc, d-GlcA and acetic acid. Fucoidans have been extensively investigated because of their various biological activities as an anticoagulant (Chevolot et al., 1999;Doane and Whistler, 1963;Nagumo and Nishino, 1996), antitumor (Itoh et al., 1993), antiviral agent (Baba et al., 1988;Ponce et al., 2003) and inhibitor of Helicobacter pylori infection (Shibata et al., 2003), and these activities differ according to linkage pattern, content of ester sulfate, sugar component and molecular weight (Berteau and Mulloy, 2003). It is also important to elucidate the structure of fucoidans to understand the interactions between their structure and biological activities. Recently, the structure of fucoidan from the brown seaweed has been identified using 2D-NMR or electrospray ionization mass spectrometry (ESI-MS) techniques (Bilan et al., 2002;Chizhov et al., 1999;Daniel et al., 2007), although there is structural diversity.In this study, Hizikia fusiformis, which is utilized for food material mainly in Japan and South Korea, was used for structural elucidation. Various reports have already addressed the preliminary characterization (Nishide et al., 1987;Nishino and Nagumo, 1987;Shiroma et al., 2003) and anticoagulant activity (Dobashi et al., 1989) of fucoidans from H. fusiformis, as well as the structure of a H. fusiformis fucoidan containing a fucose-free core (Li et al., 2006). However, the structure of fucoidan containing high-fucose and ester sulfate has not yet been reported. Here, we discuss the structure of fucoidan containing high-fucose and ester sulfate from H. fusiformis in an acidic extract. Materials and MethodsMaterials H. fusiformis used in this study was harvested in June 2002 from Yonabaru Town, Okinawa, Japan. The collected seaweed was washed with tap water and air-dried in an oven at 40 ℃ for 24 h. The dried seaweed was then powdered using a mixer (MX-620G, Matsushita Electric In-177 dustrial Co., Ltd., Japan).Isolation and fractionation of fucoidan from H. fusiformis The isolation of crude fucoidan was carried out as previously reported (Shiroma et al., 2003). Briefly, dried seaweed (20 g) was suspended in 0.1 M HCl (200 ml) and stirred at room temperature for 6 h. The suspension was filtered with a suction filter, and the fil...

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Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme

Cited by 139 publications

References 18 publications

Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Inhibition of Cell Invasion by Ethyl Alcohol Extracts of Hizikia fusiforme in AGS Human Gastric Adenocarcinoma Cells

Structural Study of Fucoidan from the Brown Seaweed Hizikia fusiformis

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