Structural investigation and response surface optimisation for improvement of kefiran production yield from a low-cost culture medium

Ghasemlou, Mehran; Khodaiyan, Faramarz; Jahanbin, Kambiz; Gharibzahedi, Seyed Mohammad Taghi; Taheri, Salman

doi:10.1016/j.foodchem.2012.01.046

Cited by 75 publications

(44 citation statements)

References 25 publications

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“…The pure PVA nanofibers exhibited various transmittance peaks at 813, 1226, 1353, 1535, 1724, 2875 and 3614 cm −1 which were attributed to the (C-C), (C-O), (CH), (CH-OAC) and (C=O) residual from primary vinyl acetate, (CH2) and free (OH) resonance, respectively, which is completely agree well with previous studies [46,47]. In kefiran nanofiber spectra, peak at 1645 cm −1 are related to bound water [48]. The sharp peak at 1020 cm −1 and finger Kf/PVA/PVP nanofiber print region at 1200-900 cm −1 is characteristic of saccharide structure and stretching modes of carbohydrate [49].The absorption peaks of Kf/PVA nanofibers appear at 2921, 2850, 1730, 1645, 1440, 1320, 1242, 1095, and 850 cm-1, which were attributed to the (CH2), (CH), (C=O) residual from primary vinyl acetate, OH bending, (CH-OH), (CH-OAC), (CH), (C-O), and (C-C) [34].…”

Section: Characterizationsupporting

confidence: 91%

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

et al. 2020

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The kefiran/polyvinyl alcohol and polyvinyl pyrrolidone nanofibers were effectively fabricated for the first time using electrospinning of poly(vinyl alcohol), poly(vinyl pyrrolidone) and kefiran blend solution. The effect of solution parameters, such as kefiran concentration and poly(vinyl alcohol)/poly(vinyl pyrrolidone) mixing ratio, were considered. The parameters' effects, such as voltage, nozzle-to-collector distance and feeding rate on nanofibers morphology, were also checked by the scanning electron microscopy images. After changing the parameters on different steps, designed by the chemo metrics software, the finest nanofibers were produced in the following conditions: 2% kefiran concentration, 30/70 kefiran/polymer mixing ratio, 12 kV voltage, 200 mm needle-to-collector distance and 0.5 (mL/h) polymer injection rate. The nanofibers produced in these conditions were uniform without knots or adhesion in the lowest diameter of 156 nm. Kefiran concentration and kefiran/polymer mixing ratio were found as the most effective parameter in the morphology and diameter size of the nanofibers. The attenuated total reflectance (ATR-Ft-IR), atomic force spectroscopy, differential scanning calorimeters and thermal gravimetric analysis were used to investigate molecular structures, threedimensional morphology and heating properties of the nanofibers, respectively.

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Section: Characterizationsupporting

confidence: 91%

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

et al. 2020

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“…Finally, the obtained sample was lyophilized after dissolving the sediment in hot distilled water (pure kefiran). 15,16,22 This sample also was stored for the quantitation of IL-6.…”

Section: Extraction Of Kefiran From Kefir Grainsmentioning

confidence: 99%

“…Kefiran has antibacterial, antifungal, and anti-tumor properties. 14,15 Antimicrobial and anti-inflammatory properties of kefir for the treatment of patients infected with one or more strains of resistant microorganism were used. 16 Kefir helps to regulate the immune system of the gastrointestinal tract (GIT).…”

Section: Introductionmentioning

confidence: 99%

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Jenab

Roghanian

Ghorbani

et al. 2020

IJN

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Background: Kefiran is a useful polysaccharide made of branched glucogalactose which is produced by microorganisms. Here the anti-MCF-7 breast cancer cells activity of kefiran and cytokine productions (IL-6) of peripheral blood mononuclear cells (PBMC) treated by kefiran was studied. Also, the effect of using kefiran as a useful and cost-effective scaffold in neural stem cell culture (PC12 cell culture) was investigated. Material and Methods: Kefiran was produced from raw milk with 0.5% fat and 10 g of kefir grains. After incubation for 48 hrs at room temperature, the solvent collected (crude kefiran). These samples were kept at 100°C for 1 hr (boiled kefiran) and the supernatant was precipitated by ethanol (pure kefiran). Then, the electrospun nanofibers, pure polyacrylonitrile (PAN), PAN/kefiran 5%, and PAN/kefiran 10% were fabricated and used as scaffolds in the cell culture. The structure of fabricated was studied by SEM and the cytokine production (IL-6) in vitro in the cell culture supernatant of PBMC line after treatment with kefiran (1mg/mL, 5 mg/mL) and kefiran-PAN 5% and 10% were carried out by enzyme-linked immunosorbent assay (ELISA). The attachment of PC12 cells was examined by inverted microscope. Also, cytotoxicity of kefiran for PC12 and MCF7 cells and morphological changes of PC12 cells were evaluated by MTT and Cresyl violet staining (Nissl staining) respectively. Results: The mean diameter of fabricated PAN/kefiran 5% and 10% nanofibers were 310.2±43.97 nm. The contact angle measurement results (26.9± 1.9 for the pure PAN scaffold vs 12.3± 1.13 for the PAN/kefiran) revealed enhanced hydrophilicity of scaffolds upon the incorporation of kefiran and PAN. Seeding of PC12 cells on the scaffolds showed that fabrication of kefiran into PAN led to the enhancement of cell attachment, proliferation, and morphological changes. Also, the promotion of PBMC growth and decreasing of MCF7 cell lines viability were shown through MTT assay. No significant changes were measured for the level of IL-6 in PAN/kefiran 5% treated cells compared to the control (p ≥ 0.05). Conclusion: These results suggest superior properties of kefiran/PAN nanofibrous scaffolds for the neural stem cell culture especially for repairing injured spinal cord. Also, the pure kefiran could be used for the enhancement of PBMC growth and reducing the MCF7 cancerous cells growth. So, using biocompatible, anti-bacterial, and anti-tumor kefiran/ PAN nanofibers for regenerative medicine seems promising.

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“…(1 → 4)-linked Gal, (1 → 4)-linked Glc and (1 → 2, 6)-linked Gal, with branch attached to O-2 of Gal residues and terminated with Glc residues (Ghasemlou, Khodaiyan, Jahanbin, Gharibzahedi, & Taheri, 2012).…”

Section: Introductionmentioning

confidence: 99%

Shear and extensional properties of kefiran

Piermaria

Bengoechea

Abraham

et al. 2016

Carbohydrate Polymers

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Structural investigation and response surface optimisation for improvement of kefiran production yield from a low-cost culture medium

Cited by 75 publications

References 25 publications

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Shear and extensional properties of kefiran

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