Structural investigation and comparative cytotoxic activity of water-soluble polysaccharides from fruit bodies of the medicinal fungus quinine conk

The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively. EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17–133 μg/mL, whereas commercial apple pectin scavenged at 0.5–2 mg/mL. The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50—0.050, 0.109 and 1.961 mg/mL), respectively. Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively. Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains. The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.

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“…The monosaccharide composition of polysaccharides was determined by gas–liquid chromatography (GLC), as described earlier [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Popov¹,

Смирнов²,

Kvashninova³

et al. 2021

Molecules

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“…The quantitative determination of phenolics was performed with the Folin–Ciocalteu reagent using gallic acid as a standard [ 67 ]. The content of neutral monosaccharides was determined by gas–liquid chromatography (GLC), as described earlier in detail [ 68 ]. The sugar concentration was determined at 490 nm using the phenol–sulfuric acid assay [ 63 ].…”

Section: Methodsmentioning

confidence: 99%

Polysaccharides of Salsola passerina: Extraction, Structural Characterization and Antioxidant Activity

Golovchenko

Popov

Смирнов

et al. 2022

IJMS

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The above-ground part of the Salsola passerine was found to contain ~13% (w/w) of polysaccharides extractable with water and aqueous solutions of ammonium oxalate and sodium carbonate. The fractions extracted with aqueous sodium carbonate solutions had the highest yield. The polysaccharides of majority fractions are characterized by similar monosaccharide composition; namely, galacturonic acid and arabinose residues are the principal components of their carbohydrate chains. The present study focused on the determination of antioxidant activity of the extracted polysaccharide fractions and elucidation of the structure of polysaccharides using nuclear magnetic resonance (NMR) spectroscopy. Homogalacturonan (HG), consisting of 1,4-linked residues of α-D-galactopyranosyluronic acid (GalpA), rhamnogalacturonan-I (RG-I), which contains a diglycosyl repeating unit with a strictly alternating sequence of 1,4-linked D-GalpA and 1,2-linked L-rhamnopyranose (Rhap) residues in the backbone, and arabinan, were identified as the structural units of the obtained polysaccharides. HMBC spectra showed that arabinan consisted of alternating regions formed by 3,5-substituted and 1,5-linked arabinofuranose residues, but there was no alternation of these residues in the arabinan structure. Polysaccharide fractions scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 0.2–1.8 mg/mL. The correlation analysis showed that the DPPH scavenging activity of polysaccharide fractions was associated with the content of phenolic compounds (PCs).

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“…Among them, lanostane triterpenes and polysaccharides have been identified to possess biological activity, such as anti-tumour, 12,13 anti-inflammatory, 14 antimicrobial, 15 neuroprotective effects, 16 and cytotoxic activity. 17 However, up to date, information on probing and identifying the hydrophilic constituents of aqueous extract of Fomes officinalis Ames (AFO), including nucleosides, dipeptides, and amino acids, remains unknown, which renders the chemical profiling of Fomes officinalis Ames incomplete. Although it has also been documented this fungus is not only known for its therapeutic qualities, it is also consumed as a tea to strengthen the immune system, 17 the composition has not been deeply explored.…”

Section: Introductionmentioning

confidence: 99%

Characterisation and identification of chemical constituents in aqueous extract of Fomes officinalis Ames based on ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry

Zhang,

Aisa,

Liu

et al. 2023

Phytochemical Analysis

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IntroductionFungal species are an attractive resource for physiologically functional food and drug precursor. Fomes officinalis Ames, a medicinal fungus, is traditionally used as a folk medicine in traditional Chinese medicine prescription for the therapy of cough and asthma. The water‐soluble substances in Chinese herbal medicines are likely to play an important physiological function. However, information on probing and identifying chemical components of the aqueous extract of Fomes officinalis Ames (AFO) remains unknown.ObjectiveThis study was conducted to screen and characterise the chemical components of AFO.Material and MethodsAn effective and sensitive ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry (UHPLC‐Q‐Orbitrap‐HRMS) method with the Full MS/PIL/dd‐MS2 acquisition approach was applied for the profiling of chemical components in AFO. An HSS T3 column was used for component separation, and a strategy of simultaneous targeted and untargeted multicomponent characterisation was implemented. Multiple identification approaches were used, including accurate molecular mass and elemental composition matching, literature and database searching, and fragmentation rules elucidation.ResultsA total of 115 components, including 20 amino acids and derivatives, six nucleobases, nine nucleosides, 75 dipeptides, two tripeptides, and three other components, were tentatively identified. Among them, the targeted exploring method screened six nucleobases and nine nucleosides including modified nucleosides. To our best knowledge, this is the first time a report has been done on the presence of the 115 compounds in AFO.ConclusionProfiling and characterisation compounds of AFO enriched its material basis, which would lay the foundation for improving potential medicinal and nutritional values and effecting comprehensive quality control of Fomes officinalis Ames.

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Structural investigation and comparative cytotoxic activity of water-soluble polysaccharides from fruit bodies of the medicinal fungus quinine conk

Cited by 15 publications

References 58 publications

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Polysaccharides of Salsola passerina: Extraction, Structural Characterization and Antioxidant Activity

Characterisation and identification of chemical constituents in aqueous extract of Fomes officinalis Ames based on ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry

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