Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein

Hothorn, Michael; Ende, Wim Van den; Lammens, Willem; Rybin, Vladimir; Scheffzek, Klaus

doi:10.1073/pnas.1004481107

Cited by 87 publications

(83 citation statements)

References 45 publications

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“…Their results suggest that the conserved amino acid motif PKF in CIF directly targets the invertase active site. This highly conserved PFK motif in the known invertase inhibitors (Hothorn et al 2010), is also conserved in the potato INH1 alleles (Pro118-Lys119-Phe120, Fig. 2).…”

Section: Allelic Variation In the Intronmentioning

confidence: 93%

“…In addition to these amino acids, the adjacent conserved Gly and Ala (Gly116 and Ala121 in Fig. 2) have been shown to allow the insertion of consecutive loop residues into the INV1 active site cleft (Hothorn et al 2010).…”

Section: Allelic Variation In the Intronmentioning

confidence: 99%

“…However, the functional signiWcance of these amino acid substitutions remains unclear and, therefore, further research is required to conWrm any alterations in protein function as a result of these amino acid substitutions. Hothorn et al (2010) studied the formation of and interaction in the complex between Arabidopsis cell wall invertase 1 (INV1) and a protein inhibitor (CIF, or cell wall inhibitor of -fructosidase) from tobacco. Their results suggest that the conserved amino acid motif PKF in CIF directly targets the invertase active site.…”

Section: Allelic Variation In the Intronmentioning

confidence: 99%

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Allele diversity for the apoplastic invertase inhibitor gene from potato

et al. 2012

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In planta the enzymatic activity of apoplastic and vacuolar invertases is controlled by inhibitory proteins. Although these invertase inhibitors (apoplastic and vacuolar forms) have been implicated as contributing to resistance to cold-induced sweetening (CIS) in tubers of potato (Solanum tuberosum L.), there is a lack of information on the structure and allelic diversity of the apoplastic invertase inhibitor genes. We have PCR-isolated and sequenced the alleles of the apoplastic invertase inhibitor gene (Stinh1) from three tetraploid potato genotypes: 1021/1 (a genotype with very high tolerance to CIS), 'Karaka' and 'Summer Delight' (two cultivars that are highly susceptible to CIS). In total, five alleles were identified in these genotypes, of which four (Stinh1-c, Stinh1-d, Stinh1-e, Stinh1-f) were novel. An analysis of allele diversity was conducted by incorporating previously published sequences of apoplastic invertase inhibitors from potato. Eight alleles were assessed for sequence polymorphism in the two exons and the single hypervariable intron. Contrary to the hypervariable intron, only 65 single nucleotide polymorphisms were observed in the exons, of which 42 confer amino acid substitutions. Phylogenetic analysis of amino acid sequences indicates that the alleles of the invertase inhibitor are highly conserved amongst members of the Solanaceae family.

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Section: Allelic Variation In the Intronmentioning

confidence: 93%

Section: Allelic Variation In the Intronmentioning

confidence: 99%

Section: Allelic Variation In the Intronmentioning

confidence: 99%

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Allele diversity for the apoplastic invertase inhibitor gene from potato

et al. 2012

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“…Evidence is emerging that not cwINVs but rather cwINV/inhibitor complexes should be considered as the main regulators of carbon partitioning in plants (Hothorn et al, 2010). Therefore, CIF was also included in our in vitro experiments studying the effects of Nin88 on NtcwINV1 activity (Fig.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Role of Defective Invertases in Plants: Tobacco Nin88 Fails to Degrade Sucrose

et al. 2013

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Cell wall invertases (cwINVs), with a high affinity for the cell wall, are fundamental enzymes in the control of plant growth, development, and carbon partitioning. Most interestingly, defective cwINVs have been described in several plant species. Their highly attenuated sucrose (Suc)-hydrolyzing capacity is due to the absence of aspartate-239 (Asp-239) and tryptophan-47 (Trp-47) homologs, crucial players for stable binding in the active site and subsequent hydrolysis. However, so far, the precise roles of such defective cwINVs remain unclear. In this paper, we report on the functional characterization of tobacco (Nicotiana tabacum) Nin88, a presumed fully active cwINV playing a crucial role during pollen development. It is demonstrated here that Nin88, lacking both Asp-239 and Trp-47 homologs, has no invertase activity. This was further supported by modeling studies and site-directed mutagenesis experiments, introducing both Asp-239 and Trp-47 homologs, leading to an enzyme with a distinct Suc-hydrolyzing capacity. In vitro experiments suggest that the addition of Nin88 counteracts the unproductive and rather aspecific binding of tobacco cwINV1 to the wall, leading to higher activities in the presence of Suc and a more efficient interaction with its cell wall inhibitor. A working model is presented based on these findings, allowing speculation on the putative role of Nin88 in muro. The results presented in this work are an important first step toward unraveling the specific roles of plant defective cwINVs.

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“…PME activity can be regulated by specific endogenous PMEIs (Sénéchal et al, 2015;Hocq et al, 2017), and PMEIs inhibit the activity of PME by forming a PME/ PMEI complex whose stability is pH dependent and higher in acidic conditions, typical of the apoplastic environment (Hothorn et al, 2004(Hothorn et al, , 2010Di Matteo et al, 2005). We suggest that specific PMEIs and/or the PMEI domain of PME34 might be involved in the regulation of PME34 activity during the HS response.…”

Section: Pme and Pg Activities Are Differentially Regulated By Heatmentioning

confidence: 99%

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

Huang

Wang

et al. 2017

Plant Physiol.

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Pectin, a major component of the primary cell wall, is synthesized in the Golgi apparatus and exported to the cell wall in a highly methylesterified form, then is partially demethylesterified by pectin methylesterases (PMEs; EC 3.1.1.11). PME activity on the status of pectin methylesterification profoundly affects the properties of pectin and, thereby, is critical for plant development and the plant defense response, although the roles of PMEs under heat stress (HS) are poorly understood. Functional genome annotation predicts that at least 66 potential PME genes are contained in Arabidopsis (Arabidopsis thaliana). Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles of PME34 (At3g49220) that both consistently showed reduced thermotolerance. Nevertheless, their impairment was independently associated with the expression of HS-responsive genes. It was also observed that PME34 transcription was induced by abscisic acid and highly expressed in guard cells. We showed that the PME34 mutation has a defect in the control of stomatal movement and greatly altered PME and polygalacturonase (EC 3.2.1.15) activity, resulting in a heat-sensitive phenotype. PME34 has a role in the regulation of transpiration through the control of the stomatal aperture due to its cell wall-modifying enzyme activity during the HS response. Hence, PME34 is required for regulating guard cell wall flexibility to mediate the heat response in Arabidopsis.

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Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein

Cited by 87 publications

References 45 publications

Allele diversity for the apoplastic invertase inhibitor gene from potato

Allele diversity for the apoplastic invertase inhibitor gene from potato

Understanding the Role of Defective Invertases in Plants: Tobacco Nin88 Fails to Degrade Sucrose

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

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