Structural insights into repression of the <i>Pneumococcal</i> fatty acid synthesis pathway by repressor FabT and co‐repressor acyl‐ACP

Zuo, Gang; Chen, Zhipeng; Jiang, Yong-Liang; Zhu, Zhongliang; Ding, Chengtao; Zhang, Zhiyong; Chen, Yuxing; Zhou, Cong‐Zhao; Li, Qiong

doi:10.1002/1873-3468.13534

Cited by 23 publications

(50 citation statements)

References 51 publications

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“…Meanwhile, oligonucleotide 21 plus oligonucleotide 22 was a palindromic sequence with high AT content, suggesting that RcrR prefers binding to DNA regions with high AT content. This preference was also similar to those of the MarR family regulators CouR ( 26 ) and FabT ( 27 ).…”

Section: Discussionsupporting

confidence: 64%

Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Gong

Chen

et al. 2021

mSystems

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Section: Discussionsupporting

confidence: 64%

Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Gong

Chen

et al. 2021

mSystems

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“…SZU445, FAS (KEGG, EC: 2.3.1.86) (total gene length: 12,443 bp; CDS length: 12,285 bp; number of amino acids: 4147; number of copies: 1) and acyl carrier protein (ACP) (COG, COG ID:COG0236) (total gene length: 429 bp; CDS length: 429 bp; number of amino acids: 143; number of copies: 1) were sequenced and annotated. In the FAS pathway, the acetic acid to long-chain saturated fatty acids (C16:0 and C18:0) step of primary fatty acid biosynthesis is catalyzed by a type I FAS, and long-chain unsaturated fatty acids are generated after a string of desaturation and elongation reactions through desaturases and elongases [27]. FAS is a multifunctional polypeptide of 4147 amino acids that includes four catalytic domains for biochemical reactions: condensation, ketoacyl reduction, hydroxyacyl dehydration, and enoyl reduction [28].…”

Section: Analysis Of Genes Involved In Long-chain Fatty Acid (Lcfa) Bmentioning

confidence: 99%

Genome Sequencing and Analysis of Thraustochytriidae sp. SZU445 Provides Novel Insights into the Polyunsaturated Fatty Acid Biosynthesis Pathway

Zhu

Liu

et al. 2020

Marine Drugs

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Thraustochytriidae sp. have broadly gained attention as a prospective resource for the production of omega-3 fatty acids production in significant quantities. In this study, the whole genome of Thraustochytriidae sp. SZU445, which produces high levels of docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA), was sequenced and subjected to protein annotation. The obtained clean reads (63.55 Mb in total) were assembled into 54 contigs and 25 scaffolds, with maximum and minimum lengths of 400 and 0.0054 Mb, respectively. A total of 3513 genes (24.84%) were identified, which could be classified into six pathways and 44 pathway groups, of which 68 genes (1.93%) were involved in lipid metabolism. In the Gene Ontology database, 22,436 genes were annotated as cellular component (8579 genes, 38.24%), molecular function (5236 genes, 23.34%), and biological process (8621 genes, 38.42%). Four enzymes corresponding to the classic fatty acid synthase (FAS) pathway and three enzymes corresponding to the classic polyketide synthase (PKS) pathway were identified in Thraustochytriidae sp. SZU445. Although PKS pathway-associated dehydratase and isomerase enzymes were not detected in Thraustochytriidae sp. SZU445, a putative DHA-and DPA-specific fatty acid pathway was identified.2 of 18 in a number of previous studies, and include the proper development of infant brain and eyes [1,2]; cardiovascular disease prevention [3]; antioxidative, anti-inflammatory [4] as well as anti-cancer properties [5], and the prevention of Alzheimer's disease [6]. Currently, ocean fish are the primary PUFA production source. However, because of the drawbacks of a fishy odor, the accumulation of contaminants, and declining fish stock, it is necessary to identify other sustainable and relatively safer alternative sources of PUFA [7].Thraustochytrids, a single-celled eukaryotic marine protist belonging to the class Labyrinthulomycetes, can accumulate large amounts of DHA, resulting in these organisms having attracted a great deal of scientific and industrial interest. Research has shown that some thraustochytrid strains can be cultivated to yield high biomasses containing substantial quantities of lipids abundant in PUFAs [8]. There is an abundance of research on the optimization of fermentation parameters in terms of salinity [9], pH, temperature [10], and cultivation medium [11] for high DHA production. Besides, metabolic engineering is also used as a promising approach to promote DHA productivity. Recent research has indicated that DHA is synthesized by two distinct pathways in thraustochytrid: the fatty acid synthase (FAS) pathway and the polyketide synthase (PKS) pathway [12]. The standard FAS pathway synthesizes fatty acids through a series of elongase-and desaturase-catalyzed reactions. Delta-4 desaturase, delta-5 desaturase, and delta-12 desaturase have been reported in Thraustochytrium aureum ATCC 34304 [13][14][15], and delta-5 elongase, delta-6 elongase, and delta-9 elongase have also been successfully identified in some thraustochytrid str...

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“…The wings of bsCggR DBD (residues 64-73) support the protein-DNA interaction by wedging into the adjacent minor grooves. This function of the wings is very often observed in wHTH proteins (Zuo et al, 2019;Fillenberg et al, 2015;Morgan et al, 2019).…”

Section: Modelling Of the Interactions With Full Dna Operatorsmentioning

confidence: 76%

Structural insight into DNA recognition by bacterial transcriptional regulators of the SorC/DeoR family

Šoltysová¹,

Sieglová²,

Fábry³

et al. 2021

Acta Cryst Sect D Struct Biol

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The SorC/DeoR family is a large family of bacterial transcription regulators that are involved in the control of carbohydrate metabolism and quorum sensing. To understand the structural basis of DNA recognition, structural studies of two functionally characterized SorC/DeoR family members from Bacillus subtilis were performed: the deoxyribonucleoside regulator bsDeoR and the central glycolytic genes regulator bsCggR. Each selected protein represents one of the subgroups that are recognized within the family. Crystal structures were determined of the N-terminal DNA-binding domains of bsDeoR and bsCggR in complex with DNA duplexes representing the minimal operator sequence at resolutions of 2.3 and 2.1 Å, respectively. While bsDeoRDBD contains a homeodomain-like HTH-type domain, bsCggRDBD contains a winged helix–turn–helix-type motif. Both proteins form C2-symmetric dimers that recognize two consecutive major grooves, and the protein–DNA interactions have been analyzed in detail. The crystal structures were used to model the interactions of the proteins with the full DNA operators, and a common mode of DNA recognition is proposed that is most likely to be shared by other members of the SorC/DeoR family.

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Structural insights into repression of the Pneumococcal fatty acid synthesis pathway by repressor FabT and co‐repressor acyl‐ACP

Cited by 23 publications

References 51 publications

Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Genome Sequencing and Analysis of Thraustochytriidae sp. SZU445 Provides Novel Insights into the Polyunsaturated Fatty Acid Biosynthesis Pathway

Structural insight into DNA recognition by bacterial transcriptional regulators of the SorC/DeoR family

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