Structural insights into hydroxycoumarin-induced apoptosis in U-937 cells

Riveiro, María E.; Moglioni, Albertina G.; Vázquez, Ramiro; Gómez, Natalia; Facorro, Graciela; Piehl, Lidia Leonor; Celis, Emilio Rubín de; Shayo, Carina; Davio, Carlos

doi:10.1016/j.bmc.2007.11.038

Cited by 159 publications

(93 citation statements)

References 35 publications

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“…Gallic acid was observed to dosedependently inhibit the growth of HL-60 promyelocytic leukaemia cells in vitro with an IC 50 value of 24 mM (4.1 mg/mL) (Madlener et al 2007). Similarly, dihydroxycoumarins esculetin (6,7-dihydroxycoumarin) and 7,8-dihydroxycoumarin have been shown to inhibit the growth of U937 leukaemia cells with IC 50 values of 31 mM (5.5 mg/mL) and 48 mM (8.6 mg/mL), respectively (Riveiro et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The green tea-derived flavanol EGCg is currently being evaluated in clinical trials as a part of combinational anticancer drug therapy (Saba et al 2015). Plant-derived coumarins have been identified as important scaffolds for anticancer therapies, particularly as lead candidates for the treatment of haematological malignancies (Riveiro et al 2008;Kaur et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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Context Pelargonium sidoides DC (Geraniaceae) is an important medicinal plant indigenous to South Africa and Lesotho. Previous studies have shown that root extracts are rich in polyphenolic compounds with antibacterial, antiviral and immunomodulatory activities. Little is known regarding the anticancer properties of Pelargonium sidoides extracts. Objective This study evaluates the anti-proliferative effects of a Pelargonium sidoides radix mother tincture (PST). Materials and methods The PST was characterized by LC-MS/MS. Anti-proliferative activity was evaluated in the pre-screen panel of the National Cancer Institute (NCI-H460, MCF-7 and SF-268) and the Jurkat leukaemia cell line at concentrations of 0-150 mg/mL. The effect on cell growth was determined with sulphorhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays after 72 h. The effect on cell cycle and apoptosis induction in Jurkat cells was determined by flow cytometry with propidium iodide and Annexin V: fluorescein isothiocyanate staining. Results Dihydroxycoumarin sulphates, gallic acid as well as gallocatechin dimers and trimers were characterized in PST by mass spectrometry. Moderate anti-proliferative effects with GI 50 values between 40 and 80 mg/mL were observed in the NCI-pre-screen panel. Strong activity observed with Jurkat cells with a GI 50 value of 6.2 mg/mL, significantly better than positive control 5-fluorouracil (GI 50 value of 9.7 mg/mL). The PST arrested Jurkat cells at the G 0 /G 1 phase of the cell cycle and increased the apoptotic cells from 9% to 21%, while the dead cells increased from 4% to 17%. Conclusion We present evidence that P. sidoides has cancer cell type-specific anti-proliferative effects and may be a source of novel anticancer molecules. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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“…Coumarin and its derivatives have shown a diverse array of biological activities including antimicrobial [17], chemotherapeutic [18,19], anticoagulant [20] and antiinflammatory [21]. A number of both naturally occurring and synthetic coumarins including aminocoumarins [22,23], hydroxycoumarins [24,25], pyranocoumarins [26], and furanocoumarins [27] have been reported to have significant antimicrobial activity against a wide range of microbes.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms

et al. 2014

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a b s t r a c tA novel series of coumarin-4-carboxylate ligands (2a-2l), their Ag(I) complexes (3a-3l) together with a number of their phenanthroline adducts (4d, 4f and 4g) have been synthesised and characterised. The ligands were prepared by either acid or base hydrolysis of their corresponding esters or by demethylation of a methylated acid derivative. The esters (1a-1i) were synthesised by an aromatic electrophilic substitution reaction between the conjugate base of substituted phenols and a vinyltriphenylphosphonium salt. The novel series of Ag(I) carboxylate complexes were prepared by reaction of silver nitrate with the coumarin ligands in aqueous/alcohol solution. The Ag(I) phenanthroline adducts were prepared by reaction of the Ag(I) complex with 1,10-phenanthroline in aqueous/alcohol solution. A selection of complexes were screened for their in vitro antibacterial activity against Pseudomonas aeruginosa grown planktonically or as a biofilm as well as a number of other clinically important strains, Escherichia coli, MRSA and Staphylococcus aureus. The activity against the P. aeruginosa biofilm was found to be significantly greater than against the planktonic bacteria.

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“…All the synthesized coumarins were thoroughly characterized using FT-IR, 1 H-NMR, 13 C-NMR and mass spectral studies. Coumarin derivatives are known for their good anti-oxidant, anti-cancer and anti-inflammatory behavior 49,50 . Hence the coumarins synthesized in the present study were evaluated for antioxidant and anticancer properties.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Novel Fatty Substituted 4-methyl-2HChromen-2-one via Cross Metathesis: Potential Antioxidants and Chemotherapeutic Agents

et al. 2016

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Structural insights into hydroxycoumarin-induced apoptosis in U-937 cells

Cited by 159 publications

References 35 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms

Synthesis of Novel Fatty Substituted 4-methyl-2HChromen-2-one via Cross Metathesis: Potential Antioxidants and Chemotherapeutic Agents

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Structural insights into hydroxycoumarin-induced apoptosis in U-937 cells

Cited by 159 publications

References 35 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms

Synthesis of Novel Fatty Substituted 4-methyl-2HChromen-2-one via Cross Metathesis: Potential Antioxidants and Chemotherapeutic Agents

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Product

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells