Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å

Seki, Satoko; Nakaniwa, Tetsuko; Castro‐Hartmann, Pablo; Sader, Kasim; Kawamoto, Akihiro; Tanaka, Hideaki; Qian, Pu; Kurisu, Genji; Fujii, Ritsuko

doi:10.1016/j.bbadva.2022.100064

Cited by 7 publications

(2 citation statements)

References 50 publications

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“…Since the ligands in the Chl binding pockets are the same in LHC II and SCP 7 the reversed Chl a to Chl b ratio hints for some structural flexibility of the SCP complex at these positions. Based on recent cryoEM data 10 the Chl a to Chl b exchange sites are assigned to the position 602, and either to positions 610 or 612, see Fig. 1, according to the numbering scheme used in 9 .…”

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confidence: 99%

Preprocess dependence of optical properties of ensembles and single siphonaxanthin-containing major antenna from the marine green alga Codium fragile

Brotosudarmo

Wittmann

Seki

et al. 2022

Sci Rep

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The siphonaxanthin-siphonein-Chl-a/b-protein (SCP) is the light-harvesting complex of the marine alga Codium fragile. Its structure resembles that of the major light-harvesting complexes of higher plants, LHC II, yet it features a reversed Chl a:Chl b ratio and it accommodates other variants of carotenoids. We have recorded the fluorescence emission spectra and fluorescence lifetimes from ensembles and single SCP complexes for three different scenarios of handling the samples. While the data obtained from ensembles of SCP complexes yield equivalent results, those obtained from single SCP complexes featured significant differences as a function of the sample history. We ascribe this discrepancy to the different excitation intensities that have been used for ensemble and single complex spectroscopy, and conclude that the SCP complexes undergo an aging process during storage. This process is manifested as a lowering of energetic barriers within the protein, enabling thermal activation of conformational changes at room temperature. This in turn leads to the preferential population of a red-shifted state that features a significant decrease of the fluorescence lifetime.

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mentioning

confidence: 99%

Preprocess dependence of optical properties of ensembles and single siphonaxanthin-containing major antenna from the marine green alga Codium fragile

Brotosudarmo

Wittmann

Seki

et al. 2022

Sci Rep

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“…i The LHCII structure of a closely related species, Codium fagile, has been published recently 64. In the structure, the switch to Chl b occurs at sites 602 and 610, which is relatively close to our predictions.…”

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confidence: 80%

Excitonic energy transfer processes in photosynthetic systems studied with two-dimensional electronic spectroscopy

Nguyen

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The response of photosynthesis to temperature and irradiance in a green alga, Ryuguphycus kuaweuweu (Ulvales) reveals adaptation to a subtidal environment in the northern Ryukyu Islands

Terada,

Shindo,

Moriyama

et al. 2023

Algal Research

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Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å

Cited by 7 publications

References 50 publications

Preprocess dependence of optical properties of ensembles and single siphonaxanthin-containing major antenna from the marine green alga Codium fragile

Preprocess dependence of optical properties of ensembles and single siphonaxanthin-containing major antenna from the marine green alga Codium fragile

Excitonic energy transfer processes in photosynthetic systems studied with two-dimensional electronic spectroscopy

The response of photosynthesis to temperature and irradiance in a green alga, Ryuguphycus kuaweuweu (Ulvales) reveals adaptation to a subtidal environment in the northern Ryukyu Islands

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