Structural insights into a unique PSI–LHCI–LHCII–Lhcb9 supercomplex from moss Physcomitrium patens

Zhang, Song; Tang, Kailu; Yan, Qiaoling; Li, Xingyue; Shen, Liangliang; Wang, Wenda; Kuang, Tingyun; Han, Guangye; Shen, Jian‐Ren; Zhang, Xing

doi:10.1038/s41477-023-01401-4

Cited by 11 publications

(2 citation statements)

References 68 publications

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“…23h ). This pigment arrangement was presumed to play a role in quenching excess energy in addition to light harvesting 38 , 39 . Intriguingly, a similar pigment arrangement was identified in AcpPCI-1, whereas the carotenoid equivalent to L3 in Lhcb9 is absent in AcpPCI-3/4 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Architecture of symbiotic dinoflagellate photosystem I–light-harvesting supercomplex in Symbiodinium

Zhao,

Wang,

et al. 2024

Nat Commun

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Symbiodinium are the photosynthetic endosymbionts for corals and play a vital role in supplying their coral hosts with photosynthetic products, forming the nutritional foundation for high-yield coral reef ecosystems. Here, we determine the cryo-electron microscopy structure of Symbiodinium photosystem I (PSI) supercomplex with a PSI core composed of 13 subunits including 2 previously unidentified subunits, PsaT and PsaU, as well as 13 peridinin-Chl a/c-binding light-harvesting antenna proteins (AcpPCIs). The PSI–AcpPCI supercomplex exhibits distinctive structural features compared to their red lineage counterparts, including extended termini of PsaD/E/I/J/L/M/R and AcpPCI-1/3/5/7/8/11 subunits, conformational changes in the surface loops of PsaA and PsaB subunits, facilitating the association between the PSI core and peripheral antennae. Structural analysis and computational calculation of excitation energy transfer rates unravel specific pigment networks in Symbiodinium PSI–AcpPCI for efficient excitation energy transfer. Overall, this study provides a structural basis for deciphering the mechanisms governing light harvesting and energy transfer in Symbiodinium PSI–AcpPCI supercomplexes adapted to their symbiotic ecosystem, as well as insights into the evolutionary diversity of PSI–LHCI among various photosynthetic organisms.

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Section: Resultsmentioning

confidence: 99%

Architecture of symbiotic dinoflagellate photosystem I–light-harvesting supercomplex in Symbiodinium

Zhao,

Wang,

et al. 2024

Nat Commun

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“…Eukaryotic photosynthetic organisms can be classified into green and red lineages 4 , the PSI cores of green lineages are conserved among green algae [5][6][7][8] , mosses [9][10][11] and higher plants 2,12,13 , and the number of bound LHCIs is correlated with their evolutionary status and environment 6 . In the case of light quality changes and treatment by NaN3/NaF, some phosphorylated LHCIIs move laterally from PSII to PSI leading to state transition and forming the unique PSI-LHCI-LHCII supercomplex [14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Structures of PSI-FCPI fromThalassiosira pseudonanain high light provide convergent evolution and light-adaptive strategies in diatom FCPIs

Feng,

Li,

Yang

et al. 2024

Preprint

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Diatoms achieve great survival success in the fluctuating oceanic environment, rely on fucoxanthin chlorophylla/c-binding proteins (FCPs) to complete light harvesting and quenching, which provide about 20% primary productivity on earth. We report two cryo-electron microscopic structures of photosystem I (PSI) with 13 or 5 FCPIs respectively at 2.78 Å and 3.20 Å resolution fromThalassiosira pseudonanaunder high light conditions. 8 Lhcr FCPIs are found detached from the PSI-13FCPI supercomplex under high light conditions, remaining 5 FCPIs are stably combined with the PSI core including Lhcr3, RedCAP, Lhcq8, Lhcf10, and FCP3 subunits. The specific pigment network in this centric diatomT. pseudonanademonstrates a higher proportion of Chlorophyllsa, diadinoxanthins, and diatoxanthins but fewer fucoxanthins compared with the huge PSI-FCPI from another centric diatomChaetoceros gracilis, thus exhibiting more efficiency in energy transfer and dissipation among FCPI antennas. These results reveal the assembly mechanism of several types of peripheral FCPIs and corresponding light-adaptive strategies inT. pseudonana, as well as the convergent evolution of the diatoms PSI-FCPI structures.

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Regulatory dynamics of the higher-plant PSI–LHCI supercomplex during state transitions

Wu,

Chen,

Wang

et al. 2023

Molecular Plant

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Structural insights into a unique PSI–LHCI–LHCII–Lhcb9 supercomplex from moss Physcomitrium patens

Cited by 11 publications

References 68 publications

Architecture of symbiotic dinoflagellate photosystem I–light-harvesting supercomplex in Symbiodinium

Architecture of symbiotic dinoflagellate photosystem I–light-harvesting supercomplex in Symbiodinium

Structures of PSI-FCPI fromThalassiosira pseudonanain high light provide convergent evolution and light-adaptive strategies in diatom FCPIs

Regulatory dynamics of the higher-plant PSI–LHCI supercomplex during state transitions

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