Structural insight into light harvesting for photosystem II in green algae

Sheng, Xin; Watanabe, Akimasa; Li, Anjie; Kim, Eun Chul; Song, Chihong; Murata, Kazuyoshi; Song, Danfeng; Minagawa, Jun; Liu, Zhongfan

doi:10.1038/s41477-019-0543-4

Cited by 135 publications

(157 citation statements)

References 48 publications

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“…In the other literature, similar bands to the A4 and A5 bands have been isolated by using α-DDM and these bands were identified as (C 2 S 2 ) 4 and (C 2 S 2 M 2 ) 2 formed by 'sandwiched' PSII-LHCII supercomplexes (23,24). However, these sandwiched complexes were absent in the isolation using amphipol (20,25). In the A3 band, a C 2 S 2 M 1type PSII-LHCII supercomplex composed of a core dimer complexes (C 2 ) with two strongly bound LHCII trimers (S 2 ) and one moderately bound LHCII trimer (M 1 ) was dominant (50%), which was consistent with the previous report in spinach (26).…”

Section: The Structure Of Monomeric and Multimeric Psii-lhcii Supercomentioning

confidence: 65%

“…Previously, we developed a procedure for purifying the PSII-LHCII supercomplex of C. reinhardtii employing A8- 35 (19). Because the obtained complex, where the detergent dodecyl-α-Dmaltoside (α-DDM) used for its solubilization were replaced by A8-35, became much more stable (19), this A8-35-substituted preparation was successfully used to determine the structure by cryo-electron microscope (20) or the binding properties of the peripheral LHCII subunits (21).…”

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confidence: 99%

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Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Kim

Watanabe

Duffy

et al. 2020

Journal of Biological Chemistry

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An intriguing molecular architecture called the “semi-crystalline photosystem II (PSII) array” has been observed in the thylakoid membranes in vascular plants. It is an array of PSII–light-harvesting complex II (LHCII) supercomplexes that only appears in low light, but its functional role has not been clarified. Here, we identified PSII–LHCII supercomplexes in their monomeric and multimeric forms in low light–acclimated spinach leaves and prepared them using sucrose-density gradient ultracentrifugation in the presence of amphipol A8-35. When the leaves were acclimated to high light, only the monomeric forms were present, suggesting that the multimeric forms represent a structural adaptation to low light and that disaggregation of the PSII–LHCII supercomplex represents an adaptation to high light. Single-particle EM revealed that the multimeric PSII–LHCII supercomplexes are composed of two (“megacomplex”) or three (“arraycomplex”) units of PSII–LHCII supercomplexes, which likely constitute a fraction of the semi-crystalline PSII array. Further characterization with fluorescence analysis revealed that multimeric forms have a higher light-harvesting capability, but a lower thermal dissipation capability than the monomeric form. These findings suggest that the configurational conversion of PSII–LHCII supercomplexes may serve as a structural basis for acclimation of plants to environmental light.

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Section: The Structure Of Monomeric and Multimeric Psii-lhcii Supercomentioning

confidence: 65%

mentioning

confidence: 99%

Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Kim

Watanabe

Duffy

et al. 2020

Journal of Biological Chemistry

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“…Table S1. Chlorophyll and carotenoid content of select Chlamydomonas photosystem II and photosystem I light-harvesting complex protein subunits as determined from their cryo-EM structures (Sheng et al, 2019;Suga et al, 2019).…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Light regulation of light‐harvesting antenna size substantially enhances photosynthetic efficiency and biomass yield in green algae^†

et al. 2020

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Summary One of the major factors limiting biomass productivity in algae is the low thermodynamic efficiency of photosynthesis. The greatest thermodynamic inefficiencies in photosynthesis occur during the conversion of light into chemical energy. At full sunlight the light‐harvesting antenna captures photons at a rate nearly 10 times faster than the rate‐limiting step in photosynthetic electron transport. Excess captured energy is dissipated by non‐productive pathways including the production of reactive oxygen species. Substantial improvements in photosynthetic efficiency have been achieved by reducing the optical cross‐section of the light‐harvesting antenna by selectively reducing chlorophyll b levels and peripheral light‐harvesting complex subunits. Smaller light‐harvesting antenna, however, may not exhibit optimal photosynthetic performance in low or fluctuating light environments. We describe a translational control system to dynamically adjust light‐harvesting antenna sizes for enhanced photosynthetic performance. By expressing a chlorophyllide a oxygenase (CAO) gene having a 5′ mRNA extension encoding a Nab1 translational repressor binding site in a CAO knockout line it was possible to continuously alter chlorophyll b levels and correspondingly light‐harvesting antenna sizes by light‐activated Nab1 repression of CAO expression as a function of growth light intensity. Significantly, algae having light‐regulated antenna sizes had substantially higher photosynthetic rates and two‐fold greater biomass productivity than the parental wild‐type strains as well as near wild‐type ability to carry out state transitions and non‐photochemical quenching. These results have broad implications for enhanced algae and plant biomass productivity.

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“…A complex with three LHCII per core was obtained from the green alga Chlamydomonas reinhardtii (e.g. (Sheng et al, 2019)), where the association of the third trimer is stronger, probably because of the absence of CP24 in this organism. However, C. reinhardtii is known to have an even larger number of LHCII per core in vivo (Drop et al, 2014).…”

Section: The Antenna Size Of Photosystem IImentioning

confidence: 99%

Beyond ‘seeing is believing’: the antenna size of the photosystems in vivo

Croce

2020

New Phytologist

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Photosystems I and II are the central components of the solar energy conversion machinery in oxygenic photosynthesis. They are large functional units embedded in the photosynthetic membranes, where they harvest light and use its energy to drive electrons from water to NADPH. Their composition and organization change in response to different environmental conditions, making these complexes dynamic units. Some of the interactions between subunits survive purification, resulting in the well-defined structures that were recently resolved by cryo-electron microscopy. Other interactions instead are weak, preventing the possibility of isolating and thus studying these complexes in vitro. This review focuses on these supercomplexes of vascular plants, which at the moment cannot be 'seen' but that represent functional units in vivo. Dedication: This review is dedicated to the memory of Prof. Jan Anderson, a pioneer in this field, a great scientist, a great woman, an example for me.

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Structural insight into light harvesting for photosystem II in green algae

Cited by 135 publications

References 48 publications

Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Light regulation of light‐harvesting antenna size substantially enhances photosynthetic efficiency and biomass yield in green algae^†

Beyond ‘seeing is believing’: the antenna size of the photosystems in vivo

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Structural insight into light harvesting for photosystem II in green algae

Cited by 135 publications

References 48 publications

Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Multimeric and monomeric photosystem II supercomplexes represent structural adaptations to low- and high-light conditions

Light regulation of light‐harvesting antenna size substantially enhances photosynthetic efficiency and biomass yield in green algae†

Beyond ‘seeing is believing’: the antenna size of the photosystems in vivo

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Light regulation of light‐harvesting antenna size substantially enhances photosynthetic efficiency and biomass yield in green algae^†