Structural insight into a molecular mechanism of methenyltetrahydrofolate cyclohydrolase from Methylobacterium extorquens AM1

Kim, Seong–Min; Lee, Seul Hoo; Kim, Il-Kwon; Seo, Hogyun; Kim, Kyoung-Jin

doi:10.1016/j.ijbiomac.2022.01.072

Cited by 1 publication

(1 citation statement)

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“…Ultimately, formyl-H 4 MPT is converted to formate. Further, some part of formate is oxidized to CO 2 by formate dehydrogenase as a dissimilation process and the other is transformed into formyl-H 4 F through formyltransferase ( fmt ) that takes part in the serine cycle as an assimilation process [ 35 , 36 ]. Recently, studies have reported that formaldehyde spontaneously combines with H 4 F; the reaction can be directly catalyzed by mtdA / fch and ftfL to generate formate and release energy at the same time.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of a Methylorubrum rhodesianum MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria

Zhang

Xia

Wang

et al. 2023

IJMS

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Methylotrophic bacteria are widely distributed in nature and can be applied in bioconversion because of their ability to use one-carbon source. The aim of this study was to investigate the mechanism underlying utilization of high methanol content and other carbon sources by Methylorubrum rhodesianum strain MB200 via comparative genomics and analysis of carbon metabolism pathway. The genomic analysis revealed that the strain MB200 had a genome size of 5.7 Mb and two plasmids. Its genome was presented and compared with that of the 25 fully sequenced strains of Methylobacterium genus. Comparative genomics revealed that the Methylorubrum strains had closer collinearity, more shared orthogroups, and more conservative MDH cluster. The transcriptome analysis of the strain MB200 in the presence of various carbon sources revealed that a battery of genes was involved in the methanol metabolism. These genes are involved in the following functions: carbon fixation, electron transfer chain, ATP energy release, and resistance to oxidation. Particularly, the central carbon metabolism pathway of the strain MB200 was reconstructed to reflect the possible reality of the carbon metabolism, including ethanol metabolism. Partial propionate metabolism involved in ethyl malonyl-CoA (EMC) pathway might help to relieve the restriction of the serine cycle. In addition, the glycine cleavage system (GCS) was observed to participate in the central carbon metabolism pathway. The study revealed the coordination of several metabolic pathways, where various carbon sources could induce associated metabolic pathways. To the best of our knowledge, this is the first study providing a more comprehensive understanding of the central carbon metabolism in Methylorubrum. This study provided a reference for potential synthetic and industrial applications of this genus and its use as chassis cells.

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Section: Resultsmentioning

confidence: 99%