Structural Heterogeneity in a Phototransformable Fluorescent Protein Impacts its Photochemical Properties
Arijit Maity,
Jip Wulffelé,
Isabel Ayala
et al.
Abstract:Photoconvertible fluorescent proteins (PCFP) are important cellular markers in advanced imaging modalities such as photoactivatable localization microscopy (PALM). However, their complex photophysical and photochemical behavior hampers applications such as quantitative and single‐particle‐tracking PALM. This work employs multidimensional NMR combined with ensemble fluorescence measurements to show that the popular mEos4b in its Green state populates two conformations (A and B), differing in side‐chain protonat… Show more
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