Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes

Ping, Jinglei; Pulsipher, Katherine W.; Vishnubhotla, Ramya; Villegas, José A.; Hicks, Tacey L.; Honig, Stephanie E.; Saven, Jeffery G.; Dmochowski, Ivan J.; Johnson, A. T. Charlie

doi:10.1039/c7sc01565h

Cited by 4 publications

(4 citation statements)

References 34 publications

(46 reference statements)

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“…Cargo smaller than the 24mer inner diameter (8 nm) can be encapsulated within the protein interior, as has been shown with other ferritins . Previously, we demonstrated the encapsulation of 6‐nm gold nanoparticles (AuNPs), where the particles nucleate AfFtn assembly via their high surface charge without the need to increase the solution ionic strength. The interior of AfFtn is negatively charged, and thus an attractive electrostatic interaction with GFP(+36) is expected.…”

Section: Introductionmentioning

confidence: 80%

A protein–protein host–guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein

et al. 2018

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We characterize the encapsulation of supercharged green fluorescent protein, GFP(+36), by thermophilic ferritin from Archaeoglobus fulgidus (AfFtn). The AfFtn-GFP(+36) assembly is rapid, nearly stoichiometric, and robust. Using a more stably assembled mutant AfFtn, we show that encapsulation can occur in the presence of mostly assembled cages, in addition to encapsulation starting from AfFtn individual subunits. Assembly and encapsulation do not occur with non-supercharged GFP or the alternately supercharged GFP(-30), highlighting the role of complementary electrostatic interactions between the cargo and AfFtn cage interior. We also present a method for verifying protein-protein encapsulation, using nickel nitrilotriacetic acid agarose resin. AfFtn-supercharged protein host-guest complexes could find applications in enzyme studies, protein separations, and in vivo protein stabilization and targeted delivery.

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Section: Introductionmentioning

confidence: 80%

A protein–protein host–guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein

et al. 2018

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“…Ferritin-based nanotherapeutics have been developed by loading ferritin nanocages with small molecules such as doxorubicin[28,29], cisplatin[30–32], curcumin[33], carotenoids[34], and cerium oxide[35]. Ferritin also provides robust strategy for metal nanoparticle encapsulation[36–38]. Strategies used drug loading include pH- or salt-induced disassembly/reassembly of ferritin, diffusion-based encapsulation, and direct conjugation of drug to ferritin surface (Fig.…”

Section: Ferritin Nanoparticlesmentioning

confidence: 99%

Ferritin-based drug delivery systems: Hybrid nanocarriers for vascular immunotargeting

Khoshnejad

Parhiz

Shuvaev

et al. 2018

Journal of Controlled Release

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109

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Ferritin subunits of heavy and light polypeptide chains self-assemble into a spherical nanocage that serves as a natural transport vehicle for metals but can include diverse cargoes. Ferritin nanoparticles are characterized by remarkable stability, small and uniform size. Chemical modifications and molecular re-engineering of ferritin yield a versatile platform of nanocarriers capable of delivering a broad range of therapeutic and imaging agents. Targeting moieties conjugated to the ferritin external surface provide multivalent anchoring of biological targets. Here, we highlight some of the current work on ferritin as well as examine potential strategies that could be used to functionalize ferritin via chemical and genetic means to enable its utility in vascular drug delivery.

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“…The furnace was then rapidly cooled to room temperature under the same flow conditions, thus allowing the graphene to form without reacting back into amorphous carbon. This process has been shown by Johnson et al to consistently yield large area high quality graphene for multiple types of applications [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Methodsmentioning

confidence: 99%

Exchange Coupling Effects on the Magnetotransport Properties of Ni-Nanoparticle-Decorated Graphene

et al. 2023

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We characterize the effect of ferromagnetic nickel nanoparticles (size ∼6 nm) on the magnetotransport properties of chemical-vapor-deposited (CVD) graphene. The nanoparticles were formed by thermal annealing of a thin Ni film evaporated on top of a graphene ribbon. The magnetoresistance was measured while sweeping the magnetic field at different temperatures, and compared against measurements performed on pristine graphene. Our results show that, in the presence of Ni nanoparticles, the usually observed zero-field peak of resistivity produced by weak localization is widely suppressed (by a factor of ∼3), most likely due to the reduction of the dephasing time as a consequence of the increase in magnetic scattering. On the other hand, the high-field magnetoresistance is amplified by the contribution of a large effective interaction field. The results are discussed in terms of a local exchange coupling, J∼6 meV, between the graphene π electrons and the 3d magnetic moment of nickel. Interestingly, this magnetic coupling does not affect the intrinsic transport parameters of graphene, such as the mobility and transport scattering rate, which remain the same with and without Ni nanoparticles, indicating that the changes in the magnetotransport properties have a purely magnetic origin.

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Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes

Abstract: Nanoparticle-driven current is measured with a graphene electrode for quantifying the pore-size of the ferritin enclosing the nanoparticle.

Cited by 4 publications

References 34 publications

A protein–protein host–guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein

A protein–protein host–guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein

Ferritin-based drug delivery systems: Hybrid nanocarriers for vascular immunotargeting

Exchange Coupling Effects on the Magnetotransport Properties of Ni-Nanoparticle-Decorated Graphene

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