Structural Features of the Algal Cell Determine Adhesion Behavior at a Charged Interface

Novosel, Nives; DeNardis, Nadica Ivošević

doi:10.1002/elan.202060580

Cited by 4 publications

(14 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surfactant activity of seawater is expressed as the equivalent amount of the nonionic synthetic surfactant Triton-X-100 (polyethylene glycol tert-octylphenyl ether) in milligram per liter. In contrast, the adhesion of fluid organic particles to the DME interface can be characterized by recording spike-shaped amperometric signals (Kovač et al 2000;Svetličić et al 2000;Ivošević DeNardis et al 2007, 2015Novosel et al 2021). Whether the adhesion is spontaneous depends on the properties of the three interfaces in contact, based on the modified Young-Dupré equation (Israelachvili 1992).…”

Section: Electrochemical Methodsmentioning

confidence: 99%

“…The video files were used as input to the open-source image processing software ICY (http:icy.bioimageanalysis.org) to analyze motility and trajectories of 1118 cells. Details of motility analysis are given in Novosel et al (2020Novosel et al ( , 2021. The software package R (R Core Team, 2020) was used for additional statistical analyses.…”

Section: Motility Analysismentioning

confidence: 99%

“…Cells of D. tertiolecta, T. suecica, and C. closterium grown at salinities of 9, 19, 27, and 38, respectively, were separated from the growth medium by centrifugation as described in the "Cell suspensions" section. Unmodified mica was used as substrate for AFM imaging of D. tertiolecta and C. closterium, whereas polyethyleneimine (PEI; Sigma-Aldrich) modified mica was used for imaging of T. suecica (Novosel et al 2021). The sample preparation protocol for AFM imaging required fixation of the D. tertiolecta suspension.…”

Section: Sample Preparation For Afm Imaging Of Cells and Released Ext...mentioning

confidence: 99%

“…The degree of hydrophobicity of the algal cell was defined as the difference between maximum adhesion values obtained for the interaction of the algal cell with untreated and CH 3 -functionalized AFM cantilevers. It was quantified with ΔW adh = W adh (no OTS) -W adh (OTS) (Novosel et al 2021).…”

Section: Young's Modulus Determinationmentioning

confidence: 99%

“…The protocol for sample preparation was recently published in Novosel et al (2021). Briefly, algal cells were immobilized on a glass coverslip coated with PEI.…”

Section: Sample Preparation For Force Spectroscopy Measurementsmentioning

confidence: 99%

See 4 more Smart Citations

Salinity-induced chemical, mechanical, and behavioral changes in marine microalgae

et al. 2022

View full text Add to dashboard Cite

This study examines how salinity reduction triggers the response of three marine microalgae at the molecular and unicellular levels in terms of chemical, mechanical, and behavioral changes. At the lowest salinity, all microalgal species exhibited an increase in membrane sterols and behaved stiffer. The glycocalyx-coated species Dunaliella tertiolecta was surrounded by a thick actin layer and showed the highest physiological activity, negatively affecting cell motility and indicating the formation of the palmella stage. The lipid content of membrane and the hydrophobicity of cell were largely preserved over a wide range of salinity, confirming the euryhaline nature of Dunaliella. The species with calcite-encrusted theca Tetraselmis suecica exhibited the highest hydrophobicity at the lowest salinity of all cells examined. At salinity of 19, the cells of T. suecica showed the lowest growth, flagellar detachment and the lowest cell speed, the highest physiological activity associated with a dense network of extracellular polymeric substances, and a decrease in membrane lipids, which could indicate develepment of cyst stage. The organosilicate encrusted species Cylindrotheca closterium appeared to be salinity tolerant. It behaved hydrophobically at lower salinity, whereas becoming hydrophilic at higher salinity, which might be related to a molecular change in the released biopolymers. This study highlighted the interplay between chemistry and mechanics that determines functional cell behavior and shows that cell surface properties and behavior could serve as stress markers for marine biota under climate change. Graphical Abstract

show abstract

Section: Electrochemical Methodsmentioning

confidence: 99%

Section: Motility Analysismentioning

confidence: 99%

Section: Sample Preparation For Afm Imaging Of Cells and Released Ext...mentioning

confidence: 99%

Section: Young's Modulus Determinationmentioning

confidence: 99%

“…The protocol for sample preparation was recently published in Novosel et al (2021). Briefly, algal cells were immobilized on a glass coverslip coated with PEI.…”

Section: Sample Preparation For Force Spectroscopy Measurementsmentioning

confidence: 99%

See 3 more Smart Citations

Salinity-induced chemical, mechanical, and behavioral changes in marine microalgae

et al. 2022

View full text Add to dashboard Cite

show abstract

Temperature-induced response in algal cell surface properties and behaviour: an experimental approach

et al. 2021

View full text Add to dashboard Cite

Microalgae are considered an accurate indicator of ecosystem perturbations induced by global climate change. The present work aims to investigate the alteration of temperature on surface properties and behaviour of three algal species using the complementary surface methods (electrochemical and atomic force microscopy). The results showed that the temperature-induced response of algae is species-specific due to the structural features of the cell envelope. Wall-less algae experience the largest nanomechanical and chemical change, while algae with silicified walls show the pronounced chemical change in the degree of hydrophobicity. Alterations of surface properties suggest a molecular modification of the algal barrier and cytoskeletal rearrangements due to a change in cell size, while algal morphology reveals no change. The physiological activity of cells showed a different organisation of released extracellular substances in the form of fine fibrillar structures, aggregated particles, and dense networks. Both types of algal responses, physiological activity, and molecular modification of the cell barrier determine the cell adhesion and motility. This study highlights the role of surface properties in cell-substrate and cell–cell interactions, which is important for the understanding of algal behaviour at natural interfaces and the mechanism of algal biofilm and aggregate formation in aquatic systems under the stress. Graphical abstract

show abstract

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

et al. 2022

Self Cite

View full text Add to dashboard Cite

Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater inflows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identified 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition.

show abstract

Structural Features of the Algal Cell Determine Adhesion Behavior at a Charged Interface

Cited by 4 publications

References 43 publications

Salinity-induced chemical, mechanical, and behavioral changes in marine microalgae

Salinity-induced chemical, mechanical, and behavioral changes in marine microalgae

Temperature-induced response in algal cell surface properties and behaviour: an experimental approach

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

Contact Info

Product

Resources

About