Structural features of incremental line-like striations in mandibular condylar cartilage of c-src-deficient mice

Shibata, Shunichi; Oda, Tsuyoshi; Abe, Tatsuhiko; Yamashita, Yasuo; Takano, Yoshiro

doi:10.1016/j.archoralbio.2006.05.009

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…We speculate that the increase in Col X expression in the mandibular condylar cartilage of ERβ KO mice is due to a decrease in osteoclasts (as seen in Figure 7). In support, accumulation of hypertrophic chondrocytes in the mandibular condyle has been reported in models of defective osteoclasts (42) .…”

Section: Discussionmentioning

confidence: 64%

“…In support, accumulation of hypertrophic chondrocytes in the mandibular condyle has been reported in models of defective osteoclasts. (42) Decreased levels of estrogen by ovariectomy resulted in increased mandibular condylar cartilage thickness and increased osteoclast numbers compared with female sham-operated controls. (16,43) The fact that we see decreased osteoclast numbers in the mandibular condyles of the ERb KO mice suggests that other estrogen-mediated pathways are involved.…”

Section: Discussionmentioning

confidence: 99%

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Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Kamiya

Chen

et al. 2012

Journal of Bone and Mineral Research

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Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via estrogen receptor beta (ERβ) inhibits axial skeletal growth in female mice. However, the role of ERβ in the mandibular condyle is largely unknown. We hypothesize that female ERβ deficient mice will have increased mandibular condylar growth compared with wild type (WT) female mice. This study examined female 7-, 49- and 120-day-old WT and ERβ knockout (KO) mice. There was a significant increase in mandibular condylar cartilage thickness, due to an increased number of cells, in the 49- and 120-day-old female ERβ KO compared with WT controls. Analysis in 49-day-old female ERβ KO mice revealed a significant increase in collagen type X, Pthrp and osteoprotegrin gene expression and a significant decrease in Rankl and Ihh gene expression, compared with WT controls. Subchondral bone analysis revealed a significant increase in total condylar volume and a decrease in the number of osteoclasts in the 49-day-old ERβ KO compared with WT female mice. There was no difference in cell proliferation in condylar cartilage between the genotypes. However, there were differences in the expression of proteins that regulate the cell cycle; we found a decrease in the expression of Tieg1 and p57 in the mandibular condylar cartilage from ERβ KO mice compared with WT mice. Taken together, our results suggest that ERβ deficiency increases condylar growth in female mice by inhibiting the fibrocartilage turnover.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Kamiya

Chen

et al. 2012

Journal of Bone and Mineral Research

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“…Formaldehyde-fixed specimens were decalcified in 10% EDTA for 7 days at 4 C, if required, and were routinely embedded in paraffin. Serial sections (6-μm thickness) were cut on the frontal or sagittal plane and stained with 0.1% alcian blue-0.1% acid fuchsin (Shibata et al, 2006a), 0.1% toluidine blue (0.1 M phosphate buffer, pH 7.4), Gomori's silver staining (Drury and Wallington, 1980), or TRAP by the hexasotized pararosaniline method (Lewinson and Silbermann, 1992;Shibata et al, 2003bShibata et al, , 2015. Some specimens were embedded in OCT compound (Sakura FineTek Japan, Tokyo, Japan), and frozen in liquid nitrogen for cryosectioning.…”

Section: Tissue Preparation and Histological/histochemical Stainingmentioning

confidence: 99%

Histochemical and Ultrastructural Study of Developing Gonial Bone With Reference to Initial Ossification of the Malleus and Reduction of Meckel's Cartilage in Mice

Shibata

Takahashi

Fujikawa

2019

The Anatomical Record

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Development of mouse gonial bone and initial ossification process of malleus were investigated. Before the formation of the gonial bone, the osteogenic area expressing alkaline phosphatase and Runx2 mRNA was widely recognized inferior to Meckel's cartilage. The gonial bone was first formed within the perichondrium at E16.0 via intramembranous ossification, surrounded the lower part of Meckel's cartilage, and then continued to extend anteriorly and medially until postnatal day (P) 3.0. At P0, multinucleated chondroclasts started to resorb the mineralized cartilage matrix with ruffled borders at the initial ossification site of the malleus (most posterior part of Meckel's cartilage). Almost all CD31-positive capillaries did not run through the gonial bone but entered the cartilage through the site where the gonial bone was not attached, indicating the forms of the initial ossification site of the malleus are similar to those at the secondary ossification center rather than the primary ossification center in the long bone. Then, the reducing process of the posterior part of Meckel's cartilage with extending gonial bone was investigated. Numerous tartrate-resistant acid phosphatase-positive mononuclear cells invaded the reducing Meckel's cartilage, and the continuity between the malleus and Meckel's cartilage was completely lost by P3.5. Both the cartilage matrix and the perichondrium were degraded, and they seemed to be incorporated into the periosteum of the gonial bone. The tensor tympani and tensor veli palatini muscles were attached to the ligament extending from the gonial bone. These findings indicated that the gonial bone has multiple functions and plays important roles in cranial formation.

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An immunohistochemical and ultrastructural study of the pericellular matrix of uneroded hypertrophic chondrocytes in the mandibular condyle of aged c-src-deficient mice

Shibata

Baba

Oda

et al. 2008

Archives of Oral Biology

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Structural features of incremental line-like striations in mandibular condylar cartilage of c-src-deficient mice

Cited by 3 publications

References 29 publications

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Histochemical and Ultrastructural Study of Developing Gonial Bone With Reference to Initial Ossification of the Malleus and Reduction of Meckel's Cartilage in Mice

An immunohistochemical and ultrastructural study of the pericellular matrix of uneroded hypertrophic chondrocytes in the mandibular condyle of aged c-src-deficient mice

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