Structural features of glycan recognition among viral pathogens

Shanker, Sreejesh; Hu, Liya; Ramani, Sasirekha; Atmar, Robert L.; Estes, Mary K.; Prasad, B. V. Venkataram

doi:10.1016/j.sbi.2017.05.007

Cited by 27 publications

(16 citation statements)

References 52 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Information on virus-carbohydrate interactions in the context of eukaryotic cells [13] may inform how viruses engage bacterial cells. In general, carbohydrate binding sites on viruses are shallow, water-exposed grooves on the virion surface, leading to weak affinity of single binding sites [14][15][16]. Viruses use multivalent interactions engaging several binding sites to recognize multiple receptor molecules on host cells.…”

Section: Molecular and Structural Determinants Of Interactions Betweementioning

confidence: 99%

Virus interactions with bacteria: Partners in the infectious dance

Neu

Mainou

2020

PLoS Pathog

View full text Add to dashboard Cite

The outcome of viral infection depends on the interplay between host factors and the environment. Host factors, like the expression of viral receptors, convey permissiveness to infection, define tropism, regulate antiviral immune responses, determine viral clearance, and spread. The host microbiota, the constellation of microbes inhabiting an organism, also plays a key role in the outcome of infection. Microbes and microbial products can directly interact with viral particles. Our understanding of how the microbiota impacts virus infection is largely limited to the bacterial component of the microbiota. Although bacteria do not support eukaryotic virus infection, they can promote viral fitness by enhancing virion stability, promoting infection of eukaryotic cells, and increasing coinfection rates. Virus binding of bacteria can also impact bacterial biology, including bacterial adherence to eukaryotic cells. These interactions can also indirectly affect the host response to viral infection. In this Pearl, we focus on how direct and indirect interactions between viruses and bacteria impact viral biology and touch on recent findings that illustrate how bacterial biology can also be impacted by interactions with eukaryotic viruses (Fig 1).

show abstract

Section: Molecular and Structural Determinants Of Interactions Betweementioning

confidence: 99%

Virus interactions with bacteria: Partners in the infectious dance

Neu

Mainou

2020

PLoS Pathog

View full text Add to dashboard Cite

show abstract

“…In addition to their multiple roles in cellular processes, these glycans also serve as target molecules for binding of pathogenic microorganisms and virulence factors, such as toxins ( 1 ). Such interactions contribute to the species specificity and tissue tropism of the pathogen; so far, this has been extensively studied mainly for viruses ( 2 ).…”

Section: Observationmentioning

confidence: 99%

Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes

Belotserkovsky

Brunner

Pinaud

et al. 2018

mBio

View full text Add to dashboard Cite

Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells. In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells. However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown. We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes. Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides). The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion. We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells. This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization. Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.

show abstract

“…Here, the first two stages are studied, which might be characterized as recognition/binding with the host cell (cf. Shanker et al, 2017;Boulant et al, 2015;Rossman, 1994) and subsequent fusion/penetration of cell or endosomal membrane (cf. Chernomordik and Kozlov, 2009;Harrison, 2008;Thorley et al, 2010;White et al, 2008;Tsai, 2007;Moyer and Nemerow, 2011).…”

Section: Introductionmentioning

confidence: 99%

Backbone Free Energy Estimator Applied to Viral Glycoproteins

Penner

2020

Journal of Computational Biology

View full text Add to dashboard Cite

Earlier analysis of the Protein Data Bank derived the distribution of rotations from the plane of a protein hydrogen bond donor peptide group to the plane of its acceptor peptide group. The quasi Boltzmann formalism of Pohl-Finkelstein is employed to estimate free energies of protein elements with these hydrogen bonds, pinpointing residues with a high propensity for conformational change. This is applied to viral glycoproteins as well as capsids, where the 90th1 percentiles of free energies determine residues that correlate well with viral fusion peptides and other functional domains in known cases and thus provide a novel method for predicting these sites of importance as antiviral drug or vaccine targets in general. The method is implemented at https://bion-server.au.dk/hbonds/ from an uploaded Protein Data Bank file.

show abstract

Structural features of glycan recognition among viral pathogens

Cited by 27 publications

References 52 publications

Virus interactions with bacteria: Partners in the infectious dance

Virus interactions with bacteria: Partners in the infectious dance

Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes

Backbone Free Energy Estimator Applied to Viral Glycoproteins

Contact Info

Product

Resources

About