Structural Features and Hormonal Regulation of the Zygotic Embryogenesis in Cereals

Круглова, Н.Н.; Seldimirova, O.A.; Zinatullina, А.Е.

doi:10.1134/s2079086420020048

Cited by 6 publications

(2 citation statements)

References 95 publications

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“…Exogenous auxin seems to be required for the establishment of a normal embryonic symmetry at the globular and early transition stages embryos since both stages were affected by manipulation of the auxin level or distribution. This dependence was confirmed by immunohistological analysis of IAA in isolated zygotic and somatic embryos of cereals cultured in vitro ( Fischer & Neuhaus, 1996 ; Przetakiewicz, Orczyk & Nadolska-Orczyk, 2003 ; Forestan, Meda & Varotto, 2010 ; Kruglova, Seldimirova & Zinatullina, 2020 ). These data emphasize that the polar transport of auxin is essential for the formation of embryonic patterns and the distribution of this hormone is at all stages of embryo development, especially with the formation of bilateral symmetry in embryos.…”

Section: Introductionmentioning

confidence: 67%

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Juzoń

Warchoł

Dziurka

et al. 2022

PeerJ

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Background Development of new cultivars is one of the vital options for adapting agriculture to climate change, and the production of doubled haploid (DH) plants can make a significant contribution to accelerating the breeding process. Oat is one of the cereals with particular health benefits, but it unfortunately still remains recalcitrant to haploidization. Our previous studies have clearly demonstrated that post-pollination with hormone treatment is a key step in haploid production through wide hybridization and indicated it as the most effective method for this species. Therefore, we subsequently addressed the problem of the influence of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on consecutive stages of DH production. Methods Twenty-nine genotypes were tested, 9,465 florets were pollinated with maize pollen 2 days after emasculation and then treated with 2,4-D at 50 mg/L and 100 mg/L. Results The applied treatments did not reveal any differences in the number of obtained haploid embryos. However, almost twice as many haploid plants formed on MS medium after applying a higher auxin concentration and 20% more successfully acclimatized. Moreover, 100 mg/L 2,4-D treatment resulted in twice as many DH lines that produced almost three times more seeds compared to 50 mg/L treatment. Nevertheless, the results have confirmed the existence of strong genotypic variation, which may significantly limit the development of an effective and economically feasible method that could be incorporated into breeding programs.

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Section: Introductionmentioning

confidence: 67%

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Juzoń

Warchoł

Dziurka

et al. 2022

PeerJ

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show abstract

“…прошлого века [5]. Анализ результатов многолетних экспериментальных исследований [6][7][8][9][10][11] позволил авторам сделать ряд теоретических обобщений [2,12] и предложить оценивать каллус in vitro как модельную систему для изучения сложнейшей проблемы морфогенеза растений [13,14].…”

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Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова¹,

Seldimirova²,

Zinatullina³

2021

TVAN

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One of the directions in plant biotechnological research is the use of callus cultures in vitro. The callus is an integrated system that is formed both exogenously (as a result of the proliferation of surface cells of various tissues of the plant organism) and endogenously (in the depth of these tissues). Initially, callus consists of homogeneous cells gradually transformed into a system of groups of heterogeneous cells with specific morphogenetic potencies. These potencies are realized by various ways of morphogenesis in vitro, including those leading to the formation of full-fledged plants-regenerants. In this review, using cereals as an example, we analyze the literature and our data on the use of calli in vitro as experimental systems for studying the plant stress-resistance to abiotic factors, especially to the drought factor. The advantages and limitations of using callus cultures in vitro are considered. The prospects of studying the mechanisms of action of abiotic stressors and resistance to them at the cellular and tissue levels under model conditions of callus culture in vitro are shown. Attention is paid to the assessment of the drought resistance of cereals under selective conditions simulating drought in vitro by such a parameter as the growth activity of calli. The issue of studying anti-stress effects in callus cultures in vitro is analyzed on the example of brassinosteroids. The prospects of using the model system “immature embryo in vivo–embryonic morphogenic callus in vitro” for rapid assessment of the effect of anti-stress plant growth regulators are considered. It is emphasized that the basis for using calli as the model system is both the significant role of the cell in all morphogenetic events of the plant organism in vivo and in vitro and the similarity of the responses of plants in vivo, calli in vitro, regenerants of callus origin in vitro and ex vitro.

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Wheat Growth, Yield, and Quality Under Water Deficit and Reduced Nitrogen Supply. A Review

Ali

Akmal

2022

Gesunde Pflanzen

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Structural Features and Hormonal Regulation of the Zygotic Embryogenesis in Cereals

Cited by 6 publications

References 95 publications

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Wheat Growth, Yield, and Quality Under Water Deficit and Reduced Nitrogen Supply. A Review

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