Structural dynamics at the berry colour locus in<i>V</i><i>itis vinifera</i> L. somatic variants

Migliaro, Daniele; Crespan, Manna; Muñoz-Organero, G.; Velasco, Riccardo; Moser, C.; Vezzulli, S.

doi:10.1111/ajgw.12103

Cited by 34 publications

(31 citation statements)

References 52 publications

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“…This region has been characterized by movement of the Gret1 retrotransposon and by hemizygosity in some genotypes due to chromosomal replacement and/or deletion (Kobayashi et al 2004, 2005; Lijavetzky et al 2006; Migliaro et al 2014; Pelsy et al 2015; Walker et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

et al. 2016

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Quantitative trait locus (QTL) identification in perennial fruit crops is impeded largely by their lengthy generation time, resulting in costly and labor-intensive maintenance of breeding programs. In a grapevine (genus Vitis) breeding program, although experimental families are typically unreplicated, the genetic backgrounds may contain similar progenitors previously selected due to their contribution of favorable alleles. In this study, we investigated the utility of joint QTL identification provided by analyzing half-sib families. The genetic control of powdery mildew was studied using two half-sib F1 families, namely GE0711/1009 (MN1264 × MN1214; N = 147) and GE1025 (MN1264 × MN1246; N = 125) with multiple species in their ancestry. Maternal genetic maps consisting of 1077 and 1641 single nucleotide polymorphism (SNP) markers, respectively, were constructed using a pseudo-testcross strategy. Ratings of field resistance to powdery mildew were obtained based on whole-plant evaluation of disease severity. This 2-year analysis uncovered two QTLs that were validated on a consensus map in these half-sib families with improved precision relative to the parental maps. Examination of haplotype combinations based on the two QTL regions identified strong association of haplotypes inherited from ‘Seyval blanc’, through MN1264, with powdery mildew resistance. This investigation also encompassed the use of microsatellite markers to establish a correlation between 206-bp (UDV-015b) and 357-bp (VViv67) fragment sizes with resistance-carrying haplotypes. Our work is one of the first reports in grapevine demonstrating the use of SNP-based maps and haplotypes for QTL identification and tagging of powdery mildew resistance in half-sib families.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-016-0586-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

et al. 2016

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“…In other cases, red-berried variants emerged as new functional MYBA genes resulting from the recombination of nonfunctional homologous genes within the color locus [69]. On the other hand, black-berried cultivars heterozygous for the null allele occasionally display grape color variants with either red/gray or white berries depending on whether only the L2 or both L1 and L2 meristem cell layers (Figure 3), respectively, carry mutations at the color locus [21,[70][71][72][73]. Molecular characterization of red/gray and white berry somatic variants of Cabernet Sauvignon and Pinot Noir cultivars through Southern blots showed that the lack of berry skin anthocyanins was associated with deletion of the functional allele of the color locus [70,74].…”

Section: Berry Colormentioning

confidence: 99%

Somatic Variation and Cultivar Innovation in Grapevine

Carbonell‐Bejerano¹,

Royo²,

Mauri³

et al. 2019

Advances in Grape and Wine Biotechnology

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Paradoxically, continuous vegetative multiplication of traditional grapevine cultivars aimed to maintain cultivar attributes in this highly heterozygous species ends in the accumulation of considerable somatic variation. This variation has long contributed to cultivar adaptation and evolution under changing environmental and cultivation conditions and has also been a source of novel traits. Understanding how this somatic variation originates provides tools for genetics-assisted tracking of selected variants and breeding. Potentially, the identification of the mutations causing the observed phenotypic variation can now help to direct genome editing approaches to improve the genotype of elite traditional cultivars. Molecular characterization of somatic variants can also generate basic information helping to understand gene biological function. In this chapter, we review the state of the art on somatic variation in grapevine at phenotypic and genome sequence levels, present possible strategies for the study of this variation, and describe a few examples in which the genetic and molecular basis or very relevant grapevine traits were successfully identified.

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“…During the period of early April and late September, the average day length is 13.8 h. Phenological traits were investigated in 2013 according to Coombe [23] and Rustioni et al [24]. The layer-specific approach was performed as described by Vezzulli et al [8] based on the theory that leaf and berry skin are derived from L1 + L2 layer and berry flesh and root only from L2 layer [8, 9]. Therefore, genomic DNA of each cultivar from the same vine was extracted three times from 300–500 mg of young leaf, berry skin, berry flesh, and root, respectively, using the modified CTAB extraction protocol [25].…”

Section: Methodsmentioning

confidence: 99%

“…Spontaneous mutations in Vitis have been studied by some researchers [6–9]. These mutations could be present in the entire meristem or only a portion (chimeras) [6].…”

Section: Introductionmentioning

confidence: 99%

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Histological and Molecular Characterization of Grape Early Ripening Bud Mutant

Guo

et al. 2016

International Journal of Genomics

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An early ripening bud mutant was analyzed based on the histological, SSR, and methylation-sensitive amplified polymorphism (MSAP) analysis and a layer-specific approach was used to investigate the differentiation between the bud mutant and its parent. The results showed that the thickness of leaf spongy tissue of mutant (MT) is larger than that of wild type (WT) and the differences are significant. The mean size of cell layer L2 was increased in the mutant and the difference is significant. The genetic background of bud mutant revealed by SSR analysis is highly uniform to its parent; just the variations from VVS2 SSR marker were detected in MT. The total methylation ratio of MT is lower than that of the corresponding WT. The outside methylation ratio in MT is much less than that in WT; the average inner methylation ratio in MT is larger than that in WT. The early ripening bud mutant has certain proportion demethylation in cell layer L2. All the results suggested that cell layer L2 of the early ripening bud mutant has changed from the WT. This study provided the basis for a better understanding of the characteristic features of the early ripening bud mutant in grape.

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Structural dynamics at the berry colour locus inVitis vinifera L. somatic variants

Cited by 34 publications

References 52 publications

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

Somatic Variation and Cultivar Innovation in Grapevine

Histological and Molecular Characterization of Grape Early Ripening Bud Mutant

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