Structural diversity of the gnih/gnih receptor system in teleost: Its involvement in early development and the negative control of LH release

Li, Shuisheng; Liu, Yun; Lu, Danqi; Chen, Huapu; Huang, Xigui; Liu, Xiaochun; Meng, Zining; Lin, Haoran; Chen, Cheng

doi:10.1016/j.peptides.2010.03.003

Cited by 140 publications

(161 citation statements)

References 33 publications

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“…Considering the changes in GnIH mRNA during reproduction, we concluded that GnIH plays a negative role in regulating GtH in the common carp. The results are consistent to those for the zebrafish and goldfish (Qi et al, 2013;Zhang et al, 2010), but opposite to the results obtained for the grass puffer, tilapia, and salmon (Amano et al, 2006;Biran et al, 2014;Shahjahan et al, 2011). The differing results may be related to the protein sequence of common carp GnIH compared with those of the goldfish, zebrafish, grass puffer, and tilapia.…”

Section: Discussionsupporting

confidence: 45%

“…When goldfish were injected intraperitoneally (i.p.) with zebrafish GnIH-III peptide, the concentration of LH decreased dramatically (Zhang et al, 2010). These results indicate the negative regulation of GnIH on GtH.…”

Section: Introductionmentioning

confidence: 77%

“…However, there different kinds of GnIHRs existed. In goldfish, zebrafish and common carp, three kinds of GnIHRs were found, but grass puffer and tilapia have only one receptor (Biran et al, 2014;Qi et al, 2013;Shahjahan et al, 2011;Zhang et al, 2010). Different numbers of GnIH receptors may also contribute to different functions of GnIH in teleosts.…”

Section: Discussionmentioning

confidence: 99%

“…So far, GnIH cDNA sequences have been identified in goldfish, grass puffer, zebrafish and tilapia (Biran et al, 2014;Shahjahan et al, 2011;Sawada et al, 2002;Zhang et al, 2010). In most of these reports, GnIH peptides were named LPXRFa-1/2/3.…”

Section: Introductionmentioning

confidence: 99%

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GnIH plays a negative role in regulating GtH expression in the common carp, Cyprinus carpio L.

Wei

Cao

Chen

et al. 2016

General and Comparative Endocrinology

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a b s t r a c tIn birds and mammals, the gonadotrophin-inhibitory hormone (GnIH) effectively inhibits the expression of gonadotrophin (GtH). In teleosts, the effects of GnIH are still unclear and under much debate. The aim of this study is to evaluate the functions of GnIH/receptors of gonadotrophin-inhibitory hormone (GnIHRs) system during reproduction in the common carp, Cyprinus carpio L. We cloned the full cDNA sequences of GnIH /GnIHRs. Real-time PCR results showed that GnIH/GnIHRs were distributed extensively across the whole hypothalamus-pituitary-gonad (HPG) axis. We also examined the changes of GnIH/GnIHRs in the HPG axis during reproduction. GnIH mRNA expression was decreased to the minimum value in Apr, the spawning month, and increased immediately after the completion of reproduction. Expression pattern of GnIH during reproduction was the opposite to those of Gonadotrophin release hormone 3 (GnRH3) and luteinizing hormone (LH). Expression patterns of GnIHRs were similar to that of GnIH in the hypothalamus. In the pituitary, GnIHR2/3 peaked in March before spawning. In the ovaries, the GnIHR1 decreased to the minimum value in April, but GnIHR2/3 increased. By injection and incubation with synthesized GnIH-III peptide, we confirmed the negative influence of GnIH on mRNAs of the follicle-stimulating hormone-b and LH-b subunits in the common carp. These results show that the GnIH/GnIHRs system is involved in the negative regulation of reproduction in HPG axis of the common carp.

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Section: Discussionsupporting

confidence: 45%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

GnIH plays a negative role in regulating GtH expression in the common carp, Cyprinus carpio L.

Wei

Cao

Chen

et al. 2016

General and Comparative Endocrinology

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“…Goldfish (g) GnIH (LPXRFamide 3) exerts both stimulatory and inhibitory influences on basal goldfish gonadotrope functions in a season-dependent manner, as well as differentially modulating the effects of sGnRH-and cGnRHII-induced gonadotrope functions (Moussavi et al , 2013. On the other hand, zebrafish GnIH reduced serum LH levels in adult goldfish (Zhang et al 2010), whereas gGnIH elevated LH and FSH release from salmon pituitary cell cultures (Amano et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Seasonal effects of GnIH on basal and GnRH-induced goldfish somatotrope functions

Moussavi¹,

Wlasichuk²,

Chang³

et al. 2014

Journal of Endocrinology

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To understand how gonadotropin-inhibitory hormone (GnIH) regulates goldfish GH cell functions, we monitored GH release and expression during early, mid-, and/or late gonadal recrudescence. In vivo and in vitro responses to goldfish (g) GnIH were different, indicating direct action at the level of pituitary, as well as interactions with other neuroendocrine factors involved in GH regulation. Injection of gGnIH consistently reduced basal serum GH levels but elevated pituitary gh mRNA levels, indicating potential dissociation of GH release and synthesis. Goldfish GnRH (sGnRH and cGnRHII) injection differentially stimulated serum GH and pituitary gh mRNA levels with some seasonal differences; these responses were reduced by gGnIH. In contrast, in vitro application of gGnIH during 24-h static incubation of goldfish pituitary cells generally elevated basal GH release and attenuated sGnRH-induced changes in gh mRNA, while suppressing basal gh mRNA levels at mid-and late recrudescence but elevating them at early recrudescence. gGnIH attenuated the GH release responses to sGnRH during static incubation at early, but not at mid-and late recrudescence. In cell column perifusion experiments examining short-term GH release, gGnIH reduced the cGnRHII-and sGnRH-stimulated secretion at late recrudescence but inhibited tha action of cGnRHII only during mid-recrudescence. Interestingly, a reduction of basal GH release upon perifusion with gGnIH during late recrudescence was followed by a rebound increase in GH release upon gGnIH removal. These results indicate that gGnIH exerts complex effects on basal and GnRH-stimulated goldfish GH cell functions and can differentially affect GH release and mRNA expression in a seasonal reproductive manner.

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LPXRFa peptide system in the European sea bass: A molecular and immunohistochemical approach

Paullada-Salmerón

Cowan

Aliaga-Guerrero

et al. 2015

J of Comparative Neurology

105

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Gonadotropin-inhibitory hormone (GnIH) is a neuropeptide that suppresses reproduction in birds and mammals by inhibiting GnRH and gonadotropin secretion. GnIH orthologs with a C-terminal LPXRFamide (LPXRFa) motif have been identified in teleost fish. Although recent work also suggests its role in fish reproduction, studies are scarce and controversial, and have mainly focused on cyprinids. In this work we cloned a full-length cDNA encoding an LPXRFa precursor in the European sea bass, Dicentrarchus labrax. In contrast to other teleosts, the sea bass LPXRFa precursor contains only two putative RFamide peptides, termed sbLPXRFa1 and sbLPXRFa2. sblpxrfa transcripts were expressed predominantly in the olfactory bulbs/telencephalon, diencephalon, midbrain tegmentum, retina, and gonads. We also developed a specific antiserum against sbLPXRFa2, which revealed sbLPXRFa-immunoreactive (ir) perikarya in the olfactory bulbs-terminal nerve, ventral telencephalon, caudal preoptic area, dorsal mesencephalic tegmentum, and rostral rhombencephalon. These sbLPXRFa-ir cells profusely innervated the preoptic area, hypothalamus, optic tectum, semicircular torus, and caudal midbrain tegmentum, but conspicuous projections also reached the olfactory bulbs, ventral/dorsal telencephalon, habenula, ventral thalamus, pretectum, rostral midbrain tegmentum, posterior tuberculum, reticular formation, and viscerosensory lobe. The retina, pineal, vascular sac, and pituitary were also targets of sbLPXRFa-ir cells. In the pituitary, this innervation was observed close to follicle-stimulating hormone (FSH), luteinizing hormone (LH) and growth hormone (GH) cells. Tract-tracing retrograde labeling suggests that telencephalic and preoptic sbLPXRFa cells might represent the source of pituitary innervation. The immunohistochemical distribution of sbLPXRFa cells and fibers suggest that LPXRFa peptides might be involved in some functions as well as reproduction, such as feeding, growth, and behavior.

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Structural diversity of the gnih/gnih receptor system in teleost: Its involvement in early development and the negative control of LH release

Cited by 140 publications

References 33 publications

GnIH plays a negative role in regulating GtH expression in the common carp, Cyprinus carpio L.

GnIH plays a negative role in regulating GtH expression in the common carp, Cyprinus carpio L.

Seasonal effects of GnIH on basal and GnRH-induced goldfish somatotrope functions

LPXRFa peptide system in the European sea bass: A molecular and immunohistochemical approach

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