Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum

Ristinmaa, Amanda Sörensen; Coleman, Tom; Cesar, Leona; Weinmann, Annika Langborg; Mazurkewich, Scott; Brändén, Gisela; Hasani, Merima; Larsbrink, Johan

doi:10.1016/j.jbc.2022.101758

Cited by 11 publications

(7 citation statements)

References 54 publications

(108 reference statements)

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“…The flexibility of insertion or lid domains plays an important role in substrate binding and catalysis for many α/β hydrolases [1]. For example, conformational changes in the insertion domain are required for substrate recognition and binding in tannases [38,39], epoxide hydrolases [40], and lipases [41,42]. The insertion domain in Lh ChlE is similar to that of Lj 0536 but does not resemble that of any α/β hydrolases with known conformation dynamics.…”

Section: Resultsmentioning

confidence: 99%

The structure of a Lactobacillus helveticus chlorogenic acid esterase and the dynamics of its insertion domain provide insights into substrate binding

Omori,

Drucker,

Okumura

et al. 2023

FEBS Letters

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Chlorogenic acid esterases (ChlEs) are a useful class of enzymes that hydrolyze chlorogenic acid (CGA) into caffeic and quinic acids. ChlEs can break down CGA in foods to improve their sensory properties and release caffeic acid in the digestive system to improve the absorption of bioactive compounds. This work presents the structure, molecular dynamics, and biochemical characterization of a ChlE from Lactobacillus helveticus (Lh). Molecular dynamics simulations suggest that substrate access to the active site of LhChlE is modulated by two hairpin loops above the active site. Docking simulations and mutational analysis suggest that two residues within the loops, Gln145 and Lys164, are important for CGA binding. Lys164 provides a slight substrate preference for CGA, whereas Gln145 is required for efficient turnover. This work is the first to examine the dynamics of a bacterial ChlE and provides insights on substrate binding preference and turnover in this type of enzyme.

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Section: Resultsmentioning

confidence: 99%

The structure of a Lactobacillus helveticus chlorogenic acid esterase and the dynamics of its insertion domain provide insights into substrate binding

Omori,

Drucker,

Okumura

et al. 2023

FEBS Letters

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“…A 2 × 250 mm Dionex Carbopac PA1 column (Thermo Scientific) with a 2 × 50 mm guard column (Thermo Scientific) was used at a column temperature of 30 °C. The eluents were A – water, B – 200 mM NaOH, and C – 200 mM NaOH and 170 mM sodium acetate 47 . The samples were eluted using eluent A (0.26 mL/min) and detected using a post-column addition of eluent B (0.13 mL/min).…”

Section: Methodsmentioning

confidence: 99%

Resin acids play key roles in shaping microbial communities during degradation of spruce bark

Ristinmaa,

Tafur Rangel,

Idström

et al. 2023

Nat Commun

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The bark is the outermost defense of trees against microbial attack, largely thanks to toxicity and prevalence of extractive compounds. Nevertheless, bark decomposes in nature, though by which species and mechanisms remains unknown. Here, we have followed the development of microbial enrichments growing on spruce bark over six months, by monitoring both chemical changes in the material and performing community and metagenomic analyses. Carbohydrate metabolism was unexpectedly limited, and instead a key activity was metabolism of extractives. Resin acid degradation was principally linked to community diversification with specific bacteria revealed to dominate the process. Metagenome-guided isolation facilitated the recovery of the dominant enrichment strain in pure culture, which represents a new species (Pseudomonas abieticivorans sp. nov.), that can grow on resin acids as a sole carbon source. Our results illuminate key stages in degradation of an abundant renewable resource, and how defensive extractive compounds have major roles in shaping microbiomes.

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“…Evidence has shown that tannase is a core enzyme of intestinal microbiota (Mancheno et al, 2022). TA treatment always activates bacterial tannase to produce GA as the main downstream metabolite (Mancheno et al, 2022;Ristinmaa et al, 2022). So, next, we examined the activity of tannase in TA-treated feces.…”

Section: Ta-induced Enrichment Of Microbial Metabolite Gallic Acid Mi...mentioning

confidence: 99%

Tannins amount determines whether tannase-containing bacteria are probiotic or pathogenic in IBD

Guo

Wang

et al. 2023

Life Sci. Alliance

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The role of dietary tannin in inflammatory bowel disease (IBD) is still not clear. Therefore, we aim to study the effect of TA in the progression of IBD. Dextran sulphate sodium (DSS)–induced model was used to mimic IBD. Metagenomics and metabolomics were performed to study the alteration of intestinal microbiota and metabolites. NCM460 and THP-1 cells were used for in vitro study. The amount of TA was associated with the outcomes of DSS-induced IBD as evidenced by in vivo and in vitro studies. Metabolomic and metagenomic analyses revealed that TA-induced enrichment of microbial metabolite gallic acid (GA) was responsible for the action of TA. Mechanistically, protective dose of GA promoted colonic mucus secretion to suppress bacterial infection and that it ameliorated DSS-induced epithelial damage by inhibiting p53 signaling, whereas toxic dose of GA directly caused epithelial damage by promoting cell cycle arrest. Therapeutic experiment showed protective dose of GA-promoted recovery of DSS-induced colonic inflammation. The role of tannase-containing bacteria can be transformed under different conditions in IBD progression.

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Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum

Cited by 11 publications

References 54 publications

The structure of a Lactobacillus helveticus chlorogenic acid esterase and the dynamics of its insertion domain provide insights into substrate binding

The structure of a Lactobacillus helveticus chlorogenic acid esterase and the dynamics of its insertion domain provide insights into substrate binding

Resin acids play key roles in shaping microbial communities during degradation of spruce bark

Tannins amount determines whether tannase-containing bacteria are probiotic or pathogenic in IBD

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