Structural differences on cell wall polysaccharides of brewer's spent Saccharomyces and microarray binding profiles with immune receptors

Reis, Sofia F.; Messias, Susana; Bastos, Rita; Martins, Vítor J.; Correia, Viviana G.; Pinheiro, Benedita A.; Silva, Lisete M.; Palma, Angelina S.; Coimbra, Manuel A.; Coelho, Elisabete

doi:10.1016/j.carbpol.2022.120325

Cited by 11 publications

(11 citation statements)

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“…Aminobutyric acid controls anxiety, diabetes, stress, high blood pressure and insomnia ( Petrucci et al, 2023 ) β1,3-glucans BSY Thermal autolysis, Hot water extraction, Subcritical water by microwave-assisted extraction Food and pharmaceutical applications. ( Reis et al, 2023b ) α1,4-Glc Mannoproteins Autolyzation, centrifugation and dialysis Food production and conservation ( da Silva Araújo et al, 2014 ) …”

Section: Brewery Waste Prospectivementioning

confidence: 99%

A critical review on the biotechnological potential of Brewers’ waste: Challenges and future alternatives

Chattaraj,

Mitra,

Ganguly

et al. 2024

Current Research in Microbial Sciences

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Section: Brewery Waste Prospectivementioning

confidence: 99%

A critical review on the biotechnological potential of Brewers’ waste: Challenges and future alternatives

Chattaraj,

Mitra,

Ganguly

et al. 2024

Current Research in Microbial Sciences

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“…Polysaccharide monomeric composition was determined by the neutral sugars released by acid hydrolysis and the analysis of their alditol acetates by gas chromatography [23] using a Perkin Elmer-Clarus 400 chromatograph (PerkinElmer, Waltham, MA, USA) with a split injector (split ratio 1:60) and an FID detector, according to the method described by the same authors [6].…”

Section: Sugar Analysismentioning

confidence: 99%

“…Glycosidic linkage composition was determined by methylation analysis and analyzed by gas chromatography coupled to quadrupole mass spectrometry (GC-qMS) of the partially methylated alditol acetates (PMAA) [24] on a Shimadzu GCMS-QP2010 Ultra gas chromatograph (Shimadzu, Kyoto, Japan), equipped with a 25 m × 0.32 mm (i.d. ), 0.1 µm film thickness HT-5 aluminum clad fused silica capillary column (SGE Analytical Science, Supelco, Bellefonte, PA, USA), according to the method described by the same authors [6].…”

Section: Glycosidic Linkage Analysismentioning

confidence: 99%

“…Detailed information on their names, sources, conditions of analysis, and reported carbohydrate specificity is in Supplementary Table S2. The microarray binding analyses were performed essentially as described [26] and specifically according to the method described by the same authors [6]. The analysis performance and the slide's drying and storage were at ambient temperature.…”

Section: Microarray Binding Analysismentioning

confidence: 99%

“…The polysaccharides are mainly β-glucans linked through (β1→3)-and (β1→6)-D-Glc linkages, representing most yeast cell wall polysaccharides (55-65%), followed by mannoproteins (35-40%), glycogen (1-29%), and chitin, which account only for 1-2% of the yeast cell wall polysaccharides. Recently, the presence of (β1→4)-D-Glc linkages were also reported on the BSY cell wall structure; however, its importance to the rigidity and shape of the cell wall demonstrating linkage to the other polysaccharide structures is not clear [2,[6][7][8][9]. Mannoproteins may be N-glycosylated, composed of 90% carbohydrates and 10% protein, with between 50 and 200 residues of (α1→6)-Man linked, highly branched by (α1→2)-Man and (α1→3)-Man terminals.…”

Section: Introductionmentioning

confidence: 99%

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Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers

Reis

Martins

Bastos

et al. 2023

Foods

Self Cite

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Brewer’s spent yeast (BSY) microcapsules have a complex network of cell-wall polysaccharides that are induced by brewing when compared to the baker’s yeast (Saccharomyces cerevisiae) microcapsules. These are rich in (β1→3)-glucans and covalently linked to (α1→4)- and (β1→4)-glucans in addition to residual mannoproteins. S. cerevisiae is often used as a drug delivery system due to its immunostimulatory potential conferred by the presence of (β1→3)-glucans. Similarly, BSY microcapsules could also be used in the encapsulation of compounds or drug delivery systems with the advantage of resisting digestion conferred by (β1→4)-glucans and promoting a broader immunomodulatory response. This work aims to study the feasibility of BSY microcapsules that are the result of alkali and subcritical water extraction processes, as oral carriers for food and biomedical applications by (1) evaluating the resistance of BSY microcapsules to in vitro digestion (IVD), (2) their recognition by the human Dectin-1 immune receptor after IVD, and (3) the recognition of IVD-solubilized material by different mammalian immune receptors. IVD digested 44–63% of the material, depending on the extraction process. The non-digested material, despite some visible agglutination and deformation of the microcapsules, preserved their spherical shape and was enriched in (β1→3)-glucans. These microcapsules were all recognized by the human Dectin-1 immune receptor. The digested material was differentially recognized by a variety of lectins of the immune system related to (β1→3)-glucans, glycogen, and mannans. These results show the potential of BSY microcapsules to be used as oral carriers for food and biomedical applications.

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