Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice

Wu, Jiacai; Li, Chanyi; Bai, Leichao; Wu, Jianyong; Bo, Rui; Ye, Mingzhu; Huang, Li; Chen, Hong‐Yuan; Rui, Wen

doi:10.1016/j.ijbiomac.2021.04.055

Cited by 48 publications

(27 citation statements)

References 43 publications

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“…For instance, both mannose and arabinose treatment alleviated DSS-induced colitis by suppressing the inflammatory response and improving barrier damage ( 26 – 28 ). Galactose is essential for immune system function, and Astragalus polysaccharide with higher galactose content showed a more beneficial effect on DSS-induced colitis ( 29 ). Therefore, we considered that the protective effect of PAMK against colitis may depend on mannose, arabinose and galactose.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide from Atractylodes macrocephala Koidz. ameliorates DSS-induced colitis in mice by regulating the Th17/Treg cell balance

et al. 2022

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Atractylodes macrocephala Koidz. is one of the most frequently used traditional Chinese medicines for the treatment of ulcerative colitis (UC). The beneficial effect of polysaccharide from Atractylodes macrocephala Koidz. (PAMK) on UC has been reported, while the underlying mechanism and target remain unclear. In this study, we systematically investigated the therapeutic effect and the underlying mechanism of PAMK in UC based on a mouse model of dextran sodium sulfate (DSS)-induced colitis. PAMK treatment (100 mg/kg, 200 mg/kg and 400 mg/kg) significantly ameliorated DSS-induced colitis, manifested as a reduction in weight loss, disease activity index (DAI), colon shortening, spleen index and histological score. Moreover, PAMK treatment inhibited inflammation and improved the integrity of the intestinal barrier in colitis mice. Mechanistically, microarray analysis determined the critical role of the immunoregulatory effect of PAMK in alleviating UC. Flow cytometry analysis further demonstrated that PAMK treatment regulated the balance between T helper (Th) 17 and regulatory T (Treg) cells in the mesenteric lymph nodes (MLN) and spleen in mice with colitis. In addition, PAMK treatment downregulated the expression of IL-6 and suppressed the phosphorylation of STAT3. Together, these data revealed that PAMK treatment alleviated DSS-induced colitis by regulating the Th17/Treg cell balance, which may be dependent on the inhibition of the IL-6/STAT3 signaling pathway. Our study is the first to elucidate that the underlying mechanism by which PAMK treatment alleviates DSS-induced colitis is associated with an improved the Th17/Treg cell balance. Collectively, the study provides evidence for the potential of PAMK to treat UC.

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Section: Discussionmentioning

confidence: 99%

Polysaccharide from Atractylodes macrocephala Koidz. ameliorates DSS-induced colitis in mice by regulating the Th17/Treg cell balance

et al. 2022

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“…The components of different extraction methods are also slightly different, including mannose, glucose, xylose, arabinose, galactose, glucuronic acid, and rhamnose ( 42 , 44 ). APS has many beneficial properties, such as anti-tumor ( 44 ), antioxidant ( 45 ), immunomodulatory ( 46 , 47 ), anti-inflammatory ( 48 ), hypoglycemic ( 49 ), and other biological activities ( Table 2 ). At present, the immunomodulatory function of APS has received increasing research attention, especially in poultry breeding ( Figure 2 ).…”

Section: Plant Polysaccharidementioning

confidence: 99%

Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides

Zhao

Yang

Bai³

et al. 2023

Front. Vet. Sci.

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With the rapid development of poultry industry and the highly intensive production management, there are an increasing number of stress factors in poultry production. Excessive stress will affect their growth and development, immune function, and induce immunosuppression, susceptibility to a variety of diseases, and even death. In recent years, increasing interest has focused on natural components extracted from plants, among which plant polysaccharides have been highlighted because of their various biological activities. Plant polysaccharides are natural immunomodulators that can promote the growth of immune organs, activate immune cells and the complement system, and release cytokines. As a green feed additive, plant polysaccharides can not only relieve stress and enhance the immunity and disease resistance of poultry, but also regulate the balance of intestinal microorganisms and effectively alleviate all kinds of stress faced by poultry. This paper reviews the immunomodulatory effects and molecular mechanisms of different plant polysaccharides (Atractylodes macrocephala Koidz polysaccharide, Astragalus polysaccharides, Taishan Pinus massoniana pollen polysaccharide, and alfalfa polysaccharide) in poultry. Current research results reveal that plant polysaccharides have potential uses as therapeutic agents for poultry immune abnormalities and related diseases.

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“…According to Leigong Paozhi Lun (a Chinese ancient book), "honey-processing allows drugs become sweet and sluggish with effects of benefiting Qi, moistening lung, relieving cough, and stopping pain and dysentery" [1]. Modern research has shown that honeyprocessing playing a synergistic role to enhance the therapeutic effect, but also moderating the medicinal properties to reduce side effects, such as honey-processed astragalus [2], honey-processed licorice [3], etc.…”

Section: Introductionmentioning

confidence: 99%

Molecular Networking, Network Pharmacology, and Molecular Docking Approaches Employed to Investigate the Changes in Ephedrae Herba before and after Honey-Processing

Guo

Ding

et al. 2022

Molecules

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Raw Ephedrae herba (REH) and honey-processed Ephedrae herba (HEH) were the different decoction pieces of Ephedrae herba (EH). Honey-processing that changes REH into HEH has been shown to relieve cough and asthma to a synergistic extent. However, the chemical markers and the synergistic mechanism of HEH need to be further studied. In this study, the ultra-high performance liquid chromatography coupled with hybrid quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) and molecular networking (MN) were used to investigate the chemical composition of REH and HEH, which led to the identification of 92 compounds. A total of 38 differential chemical markers for REH and HEH were identified using principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Network pharmacology suggests that the synergistic effect of HEH in relieving cough and asthma may be due to 31 differential chemical markers acting through 111 biological targets. Among them, four compounds and two targets probably played an important role based on the results of molecular docking. This study enriched our knowledge about the chemical composition of REH and HEH, as well as the synergistic mechanism of HEH.

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Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice

Cited by 48 publications

References 43 publications

Polysaccharide from Atractylodes macrocephala Koidz. ameliorates DSS-induced colitis in mice by regulating the Th17/Treg cell balance

Polysaccharide from Atractylodes macrocephala Koidz. ameliorates DSS-induced colitis in mice by regulating the Th17/Treg cell balance

Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides

Molecular Networking, Network Pharmacology, and Molecular Docking Approaches Employed to Investigate the Changes in Ephedrae Herba before and after Honey-Processing

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