Structural differences between hyperhydric and normal in vitro shoots of Handroanthus impetiginosus (Mart. ex DC) Mattos (Bignoniaceae)

Abstract

The anatomy of normal and hyperhydric shoots (leaves and stems) of in vitro Handroanthus impetiginosus was compared using light microscopy, scanning electron microscopy, and transmission electron microscopy. In contrast to normal shoots, hyperhydric shoots presented numerous anatomical abnormalities at the proliferation stage. Disorganized cortex, epidermal holes, epidermal discontinuity, collapsed cells, and other structural characteristics were observed in hyperhydric shoots. So, by using anatomical analysis of in vitro H. impetiginosus shoots at the proliferation stage, we can predict which plants will survive the rhizogenesis and acclimatization stages.

Plant Somatic Embryogenesis: Some Useful Considerations

“…Jausoro et al, (2010) reported disorganized cortex, epidermal holes, epidermal discontinuity, collapsed cells, and other structural characteristics were observed in hyperhydric shoots of Handroanthus impetiginosus.…”

Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

“…Anatomical and ultrastructural studies of diverse species that compared hyperhydric and normal in vitrogrown plants have been reported (Picoli et al 2008;Jausoro et al 2010).…”

“…These conditions may cause increased reserves or biomass accumulation, photosynthesis inhibition, and abnormal stomata development, which can lead to the development of hyperhydricity. Hyperhydricity hinders micropropagation and acclimatization, causing significant plant losses when vitroplants are transferred to ex vitro conditions (Jausoro et al 2010).…”

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

“…E-mail: cluna@agr.unne.edu.ar. Handroanthus impetiginosus have been limited to morphology and physiological aspects during seed germination (Amaral da Silva et al, 2004) and the physiological abnormalities due to hyperhydricity of shoots cultured in vitro (Jausoro et al, 2010). More recently, Larraburu et al (2012) reported a protocol for the micropropagation of H. impetiginosus.…”