Structural differences between Fusarium strains investigated by FT-IR spectroscopy

Abstract

The structural characteristics of Fusarium have received attention from both pure and applied scientists. Because many genes and physiological mechanisms are involved in the development of a particular structure type, this research is complicated. For revealing the structure of macromolecule in these fungal cells, FT-IR spectroscopy combined with multivariate statistical analysis was performed to characterize the structure of protein and polysaccharide of spores and mycelia obtained from different culture medium. The second derivative FT-IR spectra exhibited strain-specific infrared characteristics in the protein secondary structure sensitive amide I region. The region between 750 and 950 cm(-1) assigned to alpha- and beta-glucans was investigated for studied samples. Principal components analysis (PCA) allowed us to separate mycelia into two clusters according to different growth medium, indicating that spectra of strains may have been greatly affected by cultivation conditions.

Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: Phenotypic diversity and detection of specific markers of aggressiveness

“…The FTIR absorption spectra of F. graminearum local isolates investigated in this study, showed an overall spectral feature similar to those previously reported for this species (Fig. 2) (Nie et al, 2007a;Salman et al, 2012). Spectra were dominated by a large absorption band at 1200-900 cm −1 , the so-called window 4 (W 4 ), mainly attributed to carbohydrate vibrations (Naumann, 2000).…”

“…The chitin band, which is specific to fungi, was detected at 1151 cm − 1 and 1076 cm − 1 due to its C\ \O and C\ \C stretching vibrations (Nie et al, 2007a;Salman et al, 2012), and the glycogen C\ \O stretching vibrations were detected at the band 1028 cm −1 (Naumann, 2000). The infrared spectra associated with the fungal protein absorptions exhibited the typical amide I and amide II bands at 1640 cm −1 and 1546 cm −1 , respectively, with relatively low intensity as previously observed for Fusarium genus by Nie et al (2007a) and Salman et al (2011). The main spectral features in the high wavenumber region W 1 (between 2800 and 3000 cm − 1 ), were the bands detected at 2922 and 2853 cm −1 , assigned to the anti- Fig.…”

Synchrotron based phase contrast X-ray imaging combined with FTIR spectroscopy reveals structural and biomolecular differences in spikelets play a significant role in resistance to Fusarium in wheat

“…The amide I and II bands (1700–1485 cm −1 ), which likely arise from fungal proteins, were prominent. The fingerprint region of FHB hyphae was dominated by a broad peak near 1035 cm −1 [ 44 , 63 ]. Accordingly, the broad and strong absorption of this peak in the floret of infected cultivars in combination with a visible alteration in the protein region (α-helix turned to β-sheet) following pathogenic infection, maybe used as a signature marker for pathogenic infection with FHB.…”

Fourier transform mid-infrared spectroscopy and chemometrics to identify and discriminate Boletus edulis and Boletus tomentipes mushrooms

“…[34] According to previous literatures, the band of 1700-1000 cm −1 is the dominating region which is attributed by organic material in macrofungi. [34,35] Among them, the peak around 1650 cm −1 is mainly caused by protein amide II and the band around 1550 cm −1 belongs to protein amide II absorption. [36,37] Hence, this region of 1720-1480 cm −1 is highly relevant to protein substances.…”