Structural differences between “dark” and “bright” isolated human osteonic lamellae

Ascenzi, Maria Grazia; Ascenzi, A.; Benvenuti, A.; Burghammer, Manfred; Panzavolta, Silvia; Bigi, Adriana

doi:10.1016/s1047-8477(02)00578-6

Cited by 85 publications

(90 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Marotti (1993) indicates that the homogeneity of fibrillar orientation depends upon lamellar thickness. Ascenzi et al (2003) provide evidence that dark lamellae are dominantly composed of collagen fibers parallel with the osteon axis (corroborating the traditional view), whereas bright lamellae vary in their fiber orientation, some lying at transverse orientations but most having collagen oriented Ϯ45 degrees about the osteon axis.…”

Section: Background Preferred Collagen Fiber Orientationsupporting

confidence: 73%

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Bromage

Goldman²,

McFarlin

et al. 2003

The Anatomical Record Part B

214

211

View full text Add to dashboard Cite

Bone exhibits positive form birefringence dominated by and dependent upon the orientation of its collagen. The biomechanical efficacy of bone as a tissue is largely determined by collagen fibers of preferred orientation and distribution (and corresponding orientation of mineral crystallites), and evidence is accumulating to demonstrate that this efficacy extends to function at the organ level. This study has three aims. The first is to provide a background to the study of circularly polarized light (CPL) investigations of collagen fiber orientation in bone. The significance of preferred collagen fiber orientation in bone, linearly polarized light and CPL imaging principles, and a short history of CPL studies of mammalian functional histology are reviewed. The second is to describe, in some detail, methodological considerations relating to specimen preparation and imaging appropriate for the quantitative analysis of preferentially oriented collagen. These include section transparency, section thickness, the uniformity of the illuminating system, and CPL paraphernalia. Finally, we describe a grey-level standard useful for quantitative CPL, based upon mineralized turkey tendon, which shall be provided to investigators upon request. When due consideration is paid to specimen preparation and imaging conditions, quantitative assessment of collagen fiber orientation provides insight into the effects of mechanical loading on the skeleton. Anat Rec (Part B: New Anat) 274B: 157-168, 2003.

show abstract

Section: Background Preferred Collagen Fiber Orientationsupporting

confidence: 73%

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Bromage

Goldman²,

McFarlin

et al. 2003

The Anatomical Record Part B

214

211

View full text Add to dashboard Cite

show abstract

“…The diffracted intensity pattern from the polycrystalline aggregates hence can be useful in characterizing the spatial arrangement and orientation of the crystals. X-ray pole figure analysis is a popular tool in the texture characterization [14,[80][81][82] and the orientation patterns of collagen fibers in bone are well documented [83][84][85][86][87][88]. The texture analysis of mineral crystals using x-ray pole figure reveals that the mineral crystals are oriented not only parallel to the long axis of bone but also in other directions [80,82], and that the orientations of mineralized fibrils form a spiral around the central axis, exhibiting varying orientation angles in different lamellae [81,89].…”

Section: Orientation Of Mineral Crystalsmentioning

confidence: 99%

X-ray diffraction as a promising tool to characterize bone nanocomposites

Tadano

Giri

2011

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

To understand the characteristics of bone at the tissue level, the structure, organization and mechanical properties of the underlying levels down to the nanoscale as well as their mutual interactions need to be investigated. Such information would help understand changes in the bone properties including stiffness, strength and toughness and provide ways to assess the aged and diseased bones and the development of next generation of bio-inspired materials. X-ray diffraction techniques have gained increased interest in recent years as useful non-destructive tools for investigating the nanostructure of bone. This review provides an overview on the recent progress in this field and briefly introduces the related experimental approach. The application of x-ray diffraction to elucidating the structural and mechanical properties of mineral crystals in bone is reviewed in terms of characterization of in situ strain, residual stress-strain and crystal orientation.

show abstract

“…10 In the osteons, each vascular channel is concentrically surrounded by lamellae (2-9 mm thickness). 80 The lamellae, which are composed of bundles of collagen fibrils, have a twisted plywood arrangement, where neighboring lamellae have different fibril orientations. 80 Certain lamellae may be less organized, 81 and it is here that the osteocytes bone cells reside in lacunae (5-10 mm diameter) and interconnect through canaliculi (100-400 nm in diameter).…”

Section: Introductionmentioning

confidence: 99%

“…80 The lamellae, which are composed of bundles of collagen fibrils, have a twisted plywood arrangement, where neighboring lamellae have different fibril orientations. 80 Certain lamellae may be less organized, 81 and it is here that the osteocytes bone cells reside in lacunae (5-10 mm diameter) and interconnect through canaliculi (100-400 nm in diameter). 82,83 The fibrils (80-100 nm diameter 1 ) are surrounded by polycrystalline extrafibrillar mineral platelets (5 nm thickness, 50-80 nm width and 40-200 nm length 3 ); the extrafibrillar as well as the intrafibrillar matrix may also contain molecular components, such as non-collagenous proteins or cross-links, promoting the formation of sacrificial bonds.…”

Section: Introductionmentioning

confidence: 99%

The fracture mechanics of human bone: influence of disease and treatment

2015

View full text Add to dashboard Cite

Aging and bone diseases are associated with increased fracture risk. It is therefore pertinent to seek an understanding of the origins of such disease-related deterioration in bone's mechanical properties. The mechanical integrity of bone derives from its hierarchical structure, which in healthy tissue is able to resist complex physiological loading patterns and tolerate damage. Indeed, the mechanisms through which bone derives its mechanical properties make fracture mechanics an ideal framework to study bone's mechanical resistance, where crack-growth resistance curves give a measure of the intrinsic resistance to the initiation of cracks and the extrinsic resistance to the growth of cracks. Recent research on healthy cortical bone has demonstrated how this hierarchical structure can develop intrinsic toughness at the collagen fibril scale mainly through sliding and sacrificial bonding mechanisms that promote plasticity. Furthermore, the bone-matrix structure develops extrinsic toughness at much larger micrometer length-scales, where the structural features are large enough to resist crack growth through crack-tip shielding mechanisms. Although healthy bone tissue can generally resist physiological loading environments, certain conditions such as aging and disease can significantly increase fracture risk. In simple terms, the reduced mechanical integrity originates from alterations to the hierarchical structure. Here, we review how human cortical bone resists fracture in healthy bone and how changes to the bone structure due to aging, osteoporosis, vitamin D deficiency and Paget's disease can affect the mechanical integrity of bone tissue.

show abstract

Structural differences between “dark” and “bright” isolated human osteonic lamellae

Cited by 85 publications

References 32 publications

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Circularly polarized light standards for investigations of collagen fiber orientation in bone

X-ray diffraction as a promising tool to characterize bone nanocomposites

The fracture mechanics of human bone: influence of disease and treatment

Contact Info

Product

Resources

About