Structural determination of symbiotic nodulation factors from the broad host-range Rhizobium species NGR234

Price, N. P. J.; Talmont, Franck; Wieruszeski, Jean‐Michel; Promé, Danièlle; Promé, Jean‐Claude

doi:10.1016/0008-6215(96)00119-x

Cited by 25 publications

(12 citation statements)

References 28 publications

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“…1). These Nod factors differ from the USDA110 Nod factor in that they have a sulfate on C-3 (NGR A ) or an acetate on C-4 (NGR B ) of the 2-O-methyl-L-fucose; they are also methylated on the amide nitrogen, carbamoylated at C-6, and partially carbamoylated at C-3 or C-4 of the sugar at the nonreducing end (19). In contrast to the above Nod factors, the Nod factor from R. meliloti gave weaker inhibition when tested at equivalent concentrations ( Fig.…”

Section: Resultsmentioning

confidence: 99%

A nod factor binding lectin with apyrase activity from legume roots

Kalsi

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

151

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A lectin isolated from the roots of the legume, Dolichos biflorus, binds to Nod factors produced by rhizobial strains that nodulate this plant and has a deduced amino acid sequence with no significant homology to any lectin reported to date. This lectin also is an enzyme that catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside di-and triphosphates; the enzyme activity is increased in the presence of carbohydrate ligands. This lectin-nucleotide phosphohydrolase (LNP) has a substrate specificity characteristic of the apyrase category of phosphohydrolases, and its sequence contains four motifs characteristic of this category of enzymes. LNP is present on the surface of the root hairs, and treatment of roots with antiserum to LNP inhibits their ability to undergo root hair deformation and to form nodules on exposure to rhizobia. These properties suggest that this protein may play a role in the rhizobium-legume symbiosis and/or in a related carbohydrate recognition event endogenous to the plant.Oligosaccharide signaling events play important roles in the regulation of plant development, defense, and other interactions of plants with the environment (1-4). The establishment of the nitrogen-fixing symbiotic relationship between rhizobia and leguminous plants depends on such a signaling process. The rhizobia produce lipochitooligosaccharidic signals, called Nod factors, that elicit the differentiation of a new organ, the nodule, in the root cortex. The rhizobia bind to and invade the root hairs, where they proceed to the emerging nodule within infection threads produced by the plant (5). Both the initiation of nodule formation and rhizobial entry are host-strainspecific; this specificity is determined by the type of Nod factor produced by a particular rhizobial strain and by the ability of a leguminous species to recognize that signal.All Nod factors consist of a short, typically tetrapentameric, chitin oligosaccharidic backbone that is N-acylated at the nonreducing end, usually with a common fatty acid, such as cis-vaccenic acid. The Nod factors differ from one another in length of this backbone and the type of substituents that decorate it; these modifications determine the strain specificity of the rhizobium and depend on the set of nodulation genes possessed by that rhizobial strain (4). A related set of signals are the chitin oligosaccharides, themselves, that have been found to elicit defense responses in a wide variety of plants (for review, see ref.3).Although the structures of many of these chitooligosaccharides have been characterized, little is known about the plant proteins that bind these signals, whether as receptors for signal transduction or as binding proteins with other functions. High-affinity binding sites for chitin fragments have been found on membranous fractions prepared from tomato (7) and rice (8) suspension-cultured cells, and a 70-kDa protein that binds to chitin fragments was isolated from the rice membranes (9). Particulate fractions from roots of the legume, Medicago t...

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Section: Resultsmentioning

confidence: 99%

A nod factor binding lectin with apyrase activity from legume roots

Kalsi

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

151

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“…This allowed selective recovery of the radiolabeled monosaccharide in the aqueous layer. Thus, after treatment of N-palmitoyl [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]glucosamine with FAA II in aqueous Buffer B and the addition of sodium chloride (to 10%, w/v), selective counting of the aqueous and organic phases is an indication of the reaction progress. The salt also served the dual purpose of stopping the reaction.…”

Section: Resultsmentioning

confidence: 99%

Dictyostelium discoideum Fatty-acyl Amidase II Has Deacylase Activity on Rhizobium Nodulation Factors

Sutherland

Tobin

Rutherford

et al. 1998

Journal of Biological Chemistry

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“…Linkdue. Price et al (1992; , 1997) and are in some cases themselves also substituted with Ac or Me groups-the location of (Price et al, 1996). Interestingly, the sulfate was shown not to be substituted directly on the GlcNAc, but is linked the methyl groups is also identified on linkage analysis.…”

Section: N-and O-methyl Substituentsmentioning

confidence: 88%

“…In the FAB mass spectra of some of the LCOs from Rhizobium species strain NGR234 (Price et al, 1992;, the unsaturated fatty acid moieties. Mass spectrometry-based methods for fatty acid determination, especially for polymost prominent pseudomolecular ions are accompanied by weaker pseudomolecular species 43 and 86 mass units unsaturated chains, are still not wholly satisfactory, since they rely on several chemical handling steps that can make lower in mass.…”

Section: O-carbamoyl Substituentsmentioning

confidence: 99%