Structural classification of insecticidal proteins – Towards an in silico characterisation of novel toxins

Abstract

Article (Accepted Version) http://sro.sussex.ac.uk Berry, Colin and Crickmore, Neil (2017) Structural classification of insecticidal proteins -towards an in silico characterization of novel toxins. Journal of Invertebrate Pathology, 142. pp. 16-22. ISSN 0022-2011 This version is available from Sussex Research Online: http://sro.sussex.ac.uk/62164/ This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and reuse:Sussex Research Online is a digital repository of the research output of the University.Copyright and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable, the material made available in SRO has been checked for eligibility before being made available.Copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Accepted Manuscript Abstract:The increasing rate of discovery of new toxins with potential for the control of invertebrate pests through next generation sequencing, presents challenges for the identification of the best candidates for further development. A consideration of structural similarities between the different toxins suggest that they may be functionally less diverse than their low sequence similarities might predict. This is encouraging from the prospective of being able to use computational tools to predict toxin targets from their sequences, however more structure/function data are still required to reliably inform such predictions.Introduction:

A genome-scale metabolic reconstruction of Lysinibacillus sphaericus unveils unexploited biotechnological potentials

“…Considering that all the reviewed insecticidal factors belong to different homology groups and thus are associated with different pathogenicity mechanisms and evolutionary origins [41], we claim that L . sphaericus TL can be considered a diverse reservoir of insecticidal genes.…”

Alteration of insulin signaling to control insect pest by using transformed bacteria expressing dsRNA

“…A biopesticide, Bacillus thuringiensis (Bt), can effectively control various insect pests. 27 However, sustained use or overuse has caused Bt resistance. This issue has become serious along with widespread use of transgenic Bt crops.…”

Biocontrol Potential of a Novel Endophytic Bacterium From Mulberry (Morus) Tree

“…A variety of Bacillus species, particularly B. thuringiensis, have been reported to exhibit insecticidal potential involving several mechanisms, including the production of crystal toxins such as the Cry and Cyt proteins (Berry and Crickmore, 2017). However, to the best of our knowledge, no studies have reported any B. amyloliquefaciens strain Cry toxin genes to date.…”