Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei

Abstract

A serologically typical strain of Burkholderia pseudomallei (strain 304b) was found to produce two S-type lipopolysaccharides (LPS) differing in the chemical structures of their O-polysaccharide (O-PS) components. Structural analysis revealed that one O-antigenic polysaccharide (O-PS I) is an unbranched high-molecularweight polymer of 1,3-linked 2-O-acetyl-6-deoxy-␤-D-manno-heptopyranose residues. The other LPS O antigen (O-PS II) is an unbranched polymer of repeating disaccharide units having the structure-3)-␤-D-glucopyranose-(1-3)-6-deoxy-␣-L-talopyranose-(1in which ca. 33% of the L-6dTalp residues bear 2-O-methyl and 4-O-acetyl substituents while the other L-6dTalp residues carry only 2-O-acetyl substituents. Analysis of a serologically atypical strain of B. pseudomallei (strain 824a) produced a single LPS O-PS which was chemically identical to the 6-deoxy-D-manno-heptan O-PS I. The production of two distinct LPS raises the interesting question of their relative immunogenicities and consequently their relative importance for diagnostic serology and for the possible development of conjugate vaccines.

Use of a Safe, Reproducible, and Rapid Aerosol Delivery Method to Study Infection by Burkholderia pseudomallei and Burkholderia mallei in Mice

“…CPS and OPS are the most abundant and immunogenic molecules on the surface of B. pseudomallei and B. mallei and play critical roles in pathogenesis, as mutants lacking the polysaccharides are severely impaired in virulence [61,[69][70][71]. Both structures are highly conserved throughout these Burkholderia species [61,69,[72][73][74][75][76][77][78] and have been shown to elicit the production of antibodies in human cases of melioidosis and glanders [79][80][81][82].…”

Characterization of three capsular polysaccharides produced by Burkholderia pseudomallei

“…The LPS contained an unusual carbohydrate structure at the linkage region between lipid A and the carbohydrate moiety [5], and the structure of the whole core oligosaccharide is under investigation in our group. The chemical structure of the O-antigenic polysaccharide of the LPS was recently investigated by other groups and two types of polysaccharide were elucidated [6,7]. More recently, a capsular polysaccharide consisting of repeating units of three Dgalactose (Gal) and one 3-deoxy-D-manno-octulosonic acid (Kdo) was reported by Masoud et al [8] and Nimtz et al [9].…”

Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodies

“…Both B. mallei and B. pseudomallei are encapsulated with a polysaccharide of the same structure. The capsular polysaccharide is a homopolymer of 1,3-linked 2-O-acetyl-6-deoxy-h-d manno-heptopyranose-(1 (Perry et al, 1995;Knirel et al, 1992). All the strains used in the study had been shown to contain this capsular polysaccharide (Atkins et al, 2002a;DeShazer et al, 2001;Reckseidler et al, 2001;DeShazer et al, 1998).…”