Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach

Dey, Debayan; Tanaka, Ryouichi; Itô, Hisashi

doi:10.1007/s00239-023-10100-9

Cited by 1 publication

(1 citation statement)

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“…It is necessary to deeply dissect the specific roles of various amino acids at the C-domain in the catalytic activity and the regulation of yield and stress response in the future. Structural characterization of OsCAO1 through an in silico approach similar to AtCAO in Arabidopsis and further conducting research on OsCAO1's crystal structure and function is very valuable [42].…”

Section: Discussionmentioning

confidence: 99%

Knockout of the Chlorophyll a Oxygenase Gene OsCAO1 Reduces Chilling Tolerance in Rice Seedlings

Xiong,

Wen,

Song

et al. 2024

Genes

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Chilling stress is one of the main abiotic factors affecting rice growth and yield. In rice, chlorophyllide a oxygenase encoded by OsCAO1 is responsible for converting chlorophyllide a to chlorophyllide b, playing a crucial role in photosynthesis and thus rice growth. However, little is known about the function of OsCAO1 in chilling stress responses. The presence of the cis-acting element involved in low-temperature responsiveness (LTR) in the OsCAO1 promoter implied that OsCAO1 probably is a cold-responsive gene. The gene expression level of OsCAO1 was usually inhibited by low temperatures during the day and promoted by low temperatures at night. The OsCAO1 knockout mutants generated by the CRISPR-Cas9 technology in rice (Oryza sativa L.) exhibited significantly weakened chilling tolerance at the seedling stage. OsCAO1 dysfunction led to the accumulation of reactive oxygen species and malondialdehyde, an increase in relative electrolyte leakage, and a reduction in antioxidant gene expression under chilling stress. In addition, the functional deficiency of OsCAO1 resulted in more severe damage to chloroplast morphology, such as abnormal grana thylakoid stacking, caused by low temperatures. Moreover, the rice yield was reduced in OsCAO1 knockout mutants. Therefore, the elevated expression of OsCAO1 probably has the potential to increase both rice yield and chilling tolerance simultaneously, providing a strategy to cultivate chilling-tolerant rice varieties with high yields.

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Section: Discussionmentioning

confidence: 99%