Structural characterization of an immunostimulating polysaccharide from the stems of a new medicinal Dendrobium species: Dendrobium Taiseed Tosnobile

Yang, Li-Chan; Hsieh, Chang-Chi; Wen, Chi-Luan; Chiu, Chun‐Hui; Lin, Wen‐Chuan

doi:10.1016/j.ijbiomac.2017.05.185

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…Thus, it can be concluded that DOP can remarkably recover the balance of the splenic lymphocyte subtypes of the immunosuppressed mice. The similar phenomenon including the stimulatory effects of DOP on splenocytes were also found in other reports by MTT assays of RAW 264.7 cell line (Huang et al, 2016 (Yang et al, 2017). These results demonstrated that the two polysaccharides might affect the immune system through various mechanisms or pathways.…”

Section: Organ Indexessupporting

confidence: 89%

“…The development of effective T and B cell immunity might be stimulated by the lymphocyte proliferation response. Furthermore, polysaccharides from Dendrobium Taiseed Tosnobile (DTTP) and DHP also showed the similar effects on the T and B cell proliferation (Yang, Hsieh, Wen, Chiu, & Lin, ; Zha et al, ). The immunomodulatory effects of the three polysaccharides might be attributed to their specific structure because dextran, starch, or DHP hydrolyzates did not display any effects on spleen and liver indices, splenic and hepatic cell proliferation, or cytokine production.…”

Section: Resultsmentioning

confidence: 99%

“…The similar phenomenon including the stimulatory effects of DOP on splenocytes were also found in other reports by MTT assays of RAW 264.7 cell line (Huang et al, ). However, DTTP exhibited no marked effects on the test mice at the dosage of 300 mg/kg (Yang et al, ). These results demonstrated that the two polysaccharides might affect the immune system through various mechanisms or pathways.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

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Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

Wang

Guo

et al. 2018

J Food Process Preserv

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Dendrobium officinale (D. officinale) was regarded as the nutritious food in Asia for the remarkable bioactivities on immunmodulatory. Dendrobium officinale polysaccharide (DOP) served as the main active compound in the herb. Extraction of DOP was investigated and immunmodulatory activities were evaluated. The optimal extraction process (liquid‐to‐material ratio of 24.6:1, extraction time of 2.1 hr, and temperature of 67.4 °C) was obtained by single‐factor analysis and response surface optimization. Under this condition, DOP reached the extraction yield of 92.42%. Furthermore, DOP can promote the proliferation of splenic lymphocytes of immunodeficient mice and enhance the phagocytic capability of peritoneal macrophages. The contents of IL‐6 and TNF‐α in serum and splenocyte supernatants were significantly upregulated. Similarly, the contents of immune globulins (IgM and IgG) and hemolysis in serum was increased, as well as the antioxidant capabilities of thymus and serum. Therefore, this work gave an effective extraction method for DOP, and elucidated the immunmodulatory mechanisms of DOP at the level of the animal. Practical applications Dendrobium officinale polysaccharide (DOP) is active compounds in Dendrobium officinale (D. officinale), which was widely used as nutritious food in Asia for its remarkable bioactivities such as the curative effect on chronic atrophic gastritis and hypertension and diabetes, tumor‐suppression, and anti‐aging effects. In the current study, it optimized the extraction process to acquire high‐quality and stable DOP. Furthermore, it explored the immunoregulatory mechanism of DOP using immunosuppressive mouse model. This work contributed to the further development and utilization of DOP as a novel nutritious food additive component.

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Section: Organ Indexessupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

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Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

Wang

Guo

et al. 2018

J Food Process Preserv

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“…The raw pharmacognosy of herba dendrobii (Shi-Hu), a medicinal orchid (family: Orchidaceae), is well known; it has been widely used for health improvement as an herbal tea, and it is also included in traditional Chinese and other Asian folk medicines for its antipyretic, ophthalmic, and tonic benefits [ 1 , 2 ]. Unlike other medicinal material herbs that usually comprise only one species, herba dendrobii is prepared from several Dendrobium species—namely, D. tosaense , D. huoshanense , D. nobile, and D. candidum [ 1 , 3 , 4 ]. Herba dendrobii is highly valuable, but its growth rate is slow and its distribution in the wild is sparse; therefore, it is a protected herb before.…”

Section: Introductionmentioning

confidence: 99%

Subchronic and Genetic Safety Assessment of a New Medicinal Dendrobium Species: Dendrobium Taiseed Tosnobile in Rats

Yang

Liao

Wen

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Dendrobium Taiseed Tosnobile is a new species of herba dendrobii (Shi-Hu) that was developed by crossbreeding D. tosaense and D. nobile. Its pharmacological activity and active component have been reported, but its subchronic toxicity and genetic safety have not yet been investigated. This study assessed the 90-day oral toxicity and genetic safety of the aqueous extracts of D. Taiseed Tosnobile (DTTE) in male and female Sprague-Dawley (SD) rats. Eighty rats were divided into four groups, each consisting of ten male and ten female rats. DTTE was given orally to rats at 800, 1600, or 2400 mg/kg for 90 consecutive days, and distilled water was used for the control group. Genotoxicity studies were performed using a bacterial reverse mutation assay and in vivo mammalian cell micronucleus test in ICR mice and analyzed using flow cytometry. Throughout the study period, no abnormal changes were observed in clinical signs and body weight or on ophthalmological examinations. Additionally, no significant differences were found in urinalysis, hematology, and serum biochemistry parameters between the treatment and control groups. Necropsy and histopathological examination indicated no treatment-related changes. Based on results, the no-observed-adverse-effect level of DTTE is greater than 2400 mg/kg in SD rats.

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Current Progress on Endophytic Microbial Dynamics on Dendrobium Plants

2020

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Structural characterization of an immunostimulating polysaccharide from the stems of a new medicinal Dendrobium species: Dendrobium Taiseed Tosnobile

Cited by 17 publications

References 26 publications

Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

Subchronic and Genetic Safety Assessment of a New Medicinal Dendrobium Species: Dendrobium Taiseed Tosnobile in Rats

Current Progress on Endophytic Microbial Dynamics on Dendrobium Plants

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