Structural characterization of Alpiniae oxyphyllae fructus polysaccharide 2 and its activation effects on RAW264.7 macrophages

Yang, Xin; Zhou, Shiwei; Li, Hong; An, Jiangwen; Li, Chengheng; Zhou, Ruigang; Teng, Ling; Zhu, Yongjian; Liao, Suya; Yang, Yuhui; Chen, Huricha; Chen, Yun

doi:10.1016/j.intimp.2021.107708

Cited by 16 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polysaccharides, naturally occurring flexible macromolecular polymers with complex structures, are the primary components of plants, fungi, bacteria, algae, and even animals [ 1 ]. Polysaccharides possess numerous pharmacological activities, including anti-tumor, anti-inflammatory, antioxidant, antimicrobial, antidiabetic, immunomodulatory, etc.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

View full text Add to dashboard Cite

The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

View full text Add to dashboard Cite

show abstract

“…AOFPs signifcantly could prevent the infection of porcine epidemic diarrhea virus in IPEC-J2 cells [21]. AOFPs have signifcant immunomodulatory efects on macrophages polarizing [22]. But still now, the research studies of AOFPs on the treatment of UC have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Alpinia oxyphylla Fructus Polysaccharides Extracted with Different Solvents on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice

Ma,

Li,

Deng

et al. 2023

Journal of Food Biochemistry

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a temporary incurable inflammatory bowel disease. Alpinia oxyphylla fructus polysaccharides (AOFPs) have anti-inflammatory, antioxidant, and other biological activities. In order to explore the therapeutic effect of AOFPs on UC, this study used deionized water, NaCl, HCl, and NaOH to extract AOFPs, and their protective effects on UC were evaluated by dextran sulfate sodium-induced UC mouse model. The results showed that extraction solvents had significant effects on the yield, molecular weights, monosaccharide compositions, total sugar contents, uronic acid contents, protein contents, and surface morphology of AOFPs. Histopathological analysis found that AOFPs could significantly reduce inflammatory cell infiltration and improve crypt morphology. ELISA and real-time PCR results show that AOFPs can downregulate the contents of TNF-α, IL-1β, and IL-6 and upregulate the content of IL-10. Western blot results show that AOFPs could enhance the expression of E-cadherin, occludin, claudin 1, and ZO-1. In addition, AOFPs improved the richness and diversity of intestinal flora and reshaped the intestinal flora by increasing the proportion of beneficial bacteria and inhibiting the proportion of harmful bacteria. These results indicate that AOFPs have therapeutic effects on UC. This may be due to the regulatory effect of AOFPs on the intestinal flora, restoring intestinal inflammation and permeability.

show abstract

“…Phagocytosis is a marker of their activation. Upon activation, macrophages release NO, diverse cytokines and co-stimulatory molecules ( 23 , 24 ). Many natural polysaccharides modulate the immune system through the activation of TLR4 in macrophages ( 25 – 29 ).…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

Liu

Wang

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Atractylodes macrocephala Koidz (A. macrocephala) has been used both as a traditional medicine and functional food for hundreds of years in Asia. And it has a variety of biological activities, such as enhancing the ability of immunity and modulating effect on gastrointestinal motility. In this study, a watersoluble polysaccharide with molecular weight of 2.743 × 10 3 Da was isolated from the root of A. macrocephala. Polysaccharide from A. macrocephala (AMP) consisted of arabinose, galactose, glucose, xylose, mannose, ribose, galactose uronic acid, glucose uronic acid, with a percentage ratio of 21.86, 12.28, 34.19, 0.43, 0.92, 0.85, 28.79, and 0.67%, respectively. Zinc plays an important role in immune system. Therefore, we supposed that AMP binding with zinc oxide (ZnO) nanoparticles (AMP-ZnONPs) might be an effective immunostimulator. AMP-ZnONPs was prepared by Borch reduction, and its structural features were characterized by Scanning Electron Microscope (SEM), Transmission electron microscope (TEM), TEM-energy dispersive spectroscopy mapping (TEM-EDS mapping), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), X-ray diffraction (XRD), particle size and zeta-potential distribution analysis. Then, its immunostimulatory activity and the underlying mechanism were evaluated using RAW264.7 cells. The results showed that AMP-ZnONPs remarkably promoted cell proliferation, enhanced phagocytosis, the release of nitric oxide (NO), cytokines (IL-6 and IL-1β) and the expression of co-stimulatory molecules (CD80, CD86 and MHCII). Moreover, AMP-ZnONPs could promote Frontiers in Nutrition 01 frontiersin.org Bo et al. 10.3389/fnut.2022.992502the expression of Toll-like receptor 4 (TLR4), Myeloid differentiation factor 88 (MyD88), TNF receptor associated factor 6 (TRAF6), phospho-IκBα (P-IκBα) and phospho-p65 (P-p65), and TLR4 inhibitor (TAK242) inhibited the expression of these proteins induced by AMP-ZnONPs. Therefore, AMP-ZnONPs activated macrophages by TLR4/MyD88/NF-κB signaling pathway, indicating that AMP-ZnONPs could act as a potential immunostimulator in medicine and functional food. KEYWORDSpolysaccharide from Atractylodes macrocephala Koidz, zinc oxide nanoparticles, immunostimulatory activity, TLR4 signaling pathways, potential immunostimulator Materials and methods Reagents and materialsA. macrocephala was purchased from the Tongrentang Company in Beijing. The plant material was identified by Prof. Jingui Li. The purified AMP was prepared in our laboratory and the polysaccharide content was 96% (UV). RAW264.7 cells were obtained from American Type Culture Collection (ATCC,

show abstract

Structural characterization of Alpiniae oxyphyllae fructus polysaccharide 2 and its activation effects on RAW264.7 macrophages

Cited by 16 publications

References 28 publications

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Protective Effect of Alpinia oxyphylla Fructus Polysaccharides Extracted with Different Solvents on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

Contact Info

Product

Resources

About