Structural characterization of a water-insoluble (1→3)-α-d-glucan isolated from the Penicillium chrysogenum

Wang, Tianqi; Deng, Li; Li, Shikun; Tan, Tianwei

doi:10.1016/j.carbpol.2006.05.001

Cited by 57 publications

(28 citation statements)

References 24 publications

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“…Such α-glucans, which are not available for the enzymes commonly used in the set, can be structurally different from amylose or phytoglycogen. Linear α-1,3-glucan (pseudonigeran) as well as more complex polysaccharides including mixed α,β-glucans have been isolated from various mushrooms and microscopic fungi (Horisberger et al 1972;Kiho et al 1989;Chen et al 1998;Zhang et al 1999;Jin et al 2004;Wang et al 2007); and our preliminary investigation confirmed their presence in the fruit bodies of Pleurotus. The susceptibility of specific fungal α-glucans to enzymatic hydrolysis by α-amylase and α-glucosidase has not yet been studied, so we cannot be fully confident that the method of β-glucan assay used in this work is more effective than that used by Manzi et al (2000Manzi et al ( , 2001Manzi et al ( , 2004.…”

Section: Contents Of Glucanssupporting

confidence: 54%

Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements

Synytsya¹,

Míčková²,

Jablonský³

et al. 2008

Czech J. Food Sci.

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Synytsya A., Míčková K., Jablonský I., Sluková M., Čopíková J. (2008): Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements. Czech J. Food Sci., 26: 441-446.Fruit bodies (separately pilei and stems) of mushrooms Pleurotus ostreatus (four strains) and Pleurotus eryngii were characterised as a source of polysaccharides. The contents of glucans and dietary fibres were determined with using the respective Megazyme enzymatic kits. Enzymatic analysis of the fruit bodies confirmed significant differences in the contents of these components among the species and strains. The stems contained more insoluble dietary fibres than the pilei in all the cases and more β-glucans in most cases. However, relatively high contents of β-glucan (20-50% of dry matter) could be a result of incomplete enzymatic hydrolysis of insoluble α-1,3-glucans. Nevertheless, low food quality stems of mushrooms Pleurotus sp. could be a valuable source of cell wall glucans for the preparation of food supplements.Keywords: mushrooms Pleurotus; glucans; dietary fibres; food supplements For millennia, humankind has been valued mushrooms as an important edible and medical resource (Chang 1980;Breene 1990;Wasser 2002). The dry matter of mushroom fruit bodies is about 5-15%, they have a very low fat content and contain 19-35% proteins. Mushroom fruit bodies are rich in vitamins, mainly B 1 , B 2 , C, and D 2 (Manzi et al. 1999;Mattila et al. 2000), and contain some important elements such as K and P (Vetter 2007). The content of carbohydrates, which are mainly present as polysaccharides or glycoproteins, ranges 50-90%; the most abundant polysaccharides are chitin, α-and β-glucans and other hemicelluloses (e.g. mannans, xylans and galactans). 442Vol. 26, No. 6: 441-446 Czech J. Food Sci. 1988). Their solubility in water strongly depends on the molecular structure and conformation. Glucans bound to proteins or to chitin are usually insoluble in water. Mushroom-derived substances with antitumour, immunomodulating, and antioxidative properties are currently used as dietary supplements. Mushroom polysaccharides with antitumour action differ greatly in their chemical compositions and configurations, as well as in their physical properties. Immunomodulatory and antitumour activities are exhibited by a wide range of glycans extending from homopolymers to highly complex heteropolymers; the differences in their activities can be correlated with the solubility in water,molecular size, branching rate and form (Augustín 1998; Ooi & Lui 1999;Wasser 2002;Novak & Vetvicka 2008). Besides the well-known antitumour β-(1→3),(1→6)-glucans (Kuniak et al. 1992(Kuniak et al. , 1998, a wide range of biologically active glucans of different structures have been described. Linear or branched polysaccharides chains occur with a backbone composed of α-or β-linked glucose units, and their various side chains can be attached in different ways. The main source of biologically active polysaccharides appears to be fungal cell walls consisti...

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Section: Contents Of Glucanssupporting

confidence: 54%

Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements

Synytsya¹,

Míčková²,

Jablonský³

et al. 2008

Czech J. Food Sci.

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“…5) the main broad unresolved doublet (J H1,H2 < 3 Hz) at δ 5.531 ppm was present, which could be assigned to the α-anomeric proton of d-glucose. Any doublets with J H1,H2 ~8 Hz was observed, thus indicated the lack of β-anomeric sugars [18].…”

Section: Resultsmentioning

confidence: 99%

Fruiting bodies of Hericium erinaceus (Bull.) Pers. – a new source of water-insoluble (1→3)-α-d-glucan

et al. 2016

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A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and 1 H NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by α-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1→3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted d-Glcp represents the main branching residue of the glucan. The presence of (1→3)-α-d-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.

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“…WIG and Candida sp. lipase (EC3.1.1.3) (specific activity 28 U/mg) were made in our laboratory as described by Tan et al 17,18) Sepharose was purchased from Pharmcia (Uppsala, Sweden). BSA (!…”

Section: Methodsmentioning

confidence: 99%

“…The structural characters of this WIG have been studied. 17) In brief, it is a linear water-insoluble glucan with a molecular weight of 180 kDa. It is soluble in 0.5 M sodium hydroxide and dimethylsulfoxide.…”

mentioning

confidence: 99%

Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated fromPenicillium chrysongenum

Wang¹,

Li²,

Nie³

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Structural characterization of a water-insoluble (1→3)-α-d-glucan isolated from the Penicillium chrysogenum

Cited by 57 publications

References 24 publications

Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements

Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements

Fruiting bodies of Hericium erinaceus (Bull.) Pers. – a new source of water-insoluble (1→3)-α-d-glucan

Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated fromPenicillium chrysongenum

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