Structural characterization of a novel peptide with antimicrobial activity from the venom gland of the scorpion Tityus stigmurus: Stigmurin

Melo, Edinara Targino de; Estrela, Andréia Bergamo; Santos, Elizabeth Cristina Gomes dos; Machado, Paula Renata Lima; Farias, Kléber Juvenal Silva; Torres, Taffarel Melo; Carvalho, Enéas; Lima, Jorge; Silva-Júnior, Arnóbio Antônio da; Barbosa, Euzébio Guimarães; Fernandes-Pedrosa, Matheus de Freitas

doi:10.1016/j.peptides.2015.03.003

Cited by 51 publications

(43 citation statements)

References 82 publications

(1 reference statement)

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“…Since 2008, there have been reports for new helical AMPs from scorpions every year (Table 2). AaeAP1, Cm38, and stigmurin are the newest scorpion peptides reported in 2015 [43–45]. …”

Section: Discovery Of Spider and Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

“…They showed broad-spectrum antibacterial activity. Interestingly, ctriporin, Hp1404, and stigmurin are solely active against Gram-positive bacteria [45,79,80]. Hp1404, isolated from Heterometrus petersii , is an amphipathic α-helical peptide with activity against Gram-positive bacteria including MRSA.…”

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

“…Stigmurin was selected based on a transcriptomic analysis of the Brazilian yellow scorpion Tityus stigmurus venom gland. This newly found peptide exhibited antibacterial and antifungal activity, with MICs ranging from 8.7 to 69.5 µM [45]. …”

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

“…Meucin-13 and meucin-18 exhibited extensive cytolytic effects on fungi and yeasts [75]. Stigmurin exhibited both antibacterial and antifungal activity with MICs ranging from 8.7 to 69.5 µM [45]. The MICs of ctriporin against C. albicans are 5 to 20 µg/ml [85].…”

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

“…This is consistent with cytotoxicity obtained using other mammalian cell lines. Stigmurin caused 50% killing (IC 50 ) of human cervical cancer cells (SiHa) and African green monkey kidney epithelial (Vero E6) cell lines at 118 and 150 µM, respectively [45]. …”

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

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Insights into Antimicrobial Peptides from Spiders and Scorpions

Wang¹,

Wang

2016

PPL

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The venoms of spiders and scorpions contain a variety of chemical compounds. Antimicrobial peptides (AMPs) from these organisms were first discovered in the 1990s. As of May 2015, there were 42 spider’s and 63 scorpion’s AMPs in the Antimicrobial Peptide Database (http://aps.unmc.edu/AP). These peptides have demonstrated broad or narrow-spectrum activities against bacteria, fungi, viruses, and parasites. In addition, they can be toxic to cancer cells, insects and erythrocytes. To provide insight into such an activity spectrum, this article discusses the discovery, classification, structure and activity relationships, bioinformatics analysis, and potential applications of spider and scorpion AMPs. Our analysis reveals that, in the case of linear peptides, spiders use both glycine-rich and helical peptide models for defense, whereas scorpions use two distinct helical peptide models with different amino acid compositions to exert the observed antimicrobial activities and hemolytic toxicity. Our structural bioinformatics study improves the knowledge in the field and can be used to design more selective peptides to combat tumors, parasites, and viruses.

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“…Since 2008, there have been reports for new helical AMPs from scorpions every year (Table 2). AaeAP1, Cm38, and stigmurin are the newest scorpion peptides reported in 2015 [43–45]. …”

Section: Discovery Of Spider and Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

Section: Scorpion Antimicrobial Peptidesmentioning

confidence: 99%

See 3 more Smart Citations

Insights into Antimicrobial Peptides from Spiders and Scorpions

Wang¹,

Wang

2016

PPL

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Natural Products as Antiparasitic, Antifungal, and Antibacterial Agents

Ali,

Mishra

2024

Drugs From Nature: Targets, Assay Systems and Leads

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A Critical Review of Short Antimicrobial Peptides from Scorpion Venoms, Their Physicochemical Attributes, and Potential for the Development of New Drugs

Fong-Coronado,

Ramirez,

Quintero-Hernández

et al. 2024

J Membrane Biol

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Scorpion venoms have proven to be excellent sources of antimicrobial agents. However, although many of them have been functionally characterized, they remain underutilized as pharmacological agents, despite their evident therapeutic potential. In this review, we discuss the physicochemical properties of short scorpion venom antimicrobial peptides (ssAMPs). Being generally short (13–25 aa) and amidated, their proven antimicrobial activity is generally explained by parameters such as their net charge, the hydrophobic moment, or the degree of helicity. However, for a complete understanding of their biological activities, also considering the properties of the target membranes is of great relevance. Here, with an extensive analysis of the physicochemical, structural, and thermodynamic parameters associated with these biomolecules, we propose a theoretical framework for the rational design of new antimicrobial drugs. Through a comparison of these physicochemical properties with the bioactivity of ssAMPs in pathogenic bacteria such as Staphylococcus aureus or Acinetobacter baumannii, it is evident that in addition to the net charge, the hydrophobic moment, electrostatic energy, or intrinsic flexibility are determining parameters to understand their performance. Although the correlation between these parameters is very complex, the consensus of our analysis suggests that there is a delicate balance between them and that modifying one affects the rest. Understanding the contribution of lipid composition to their bioactivities is also underestimated, which suggests that for each peptide, there is a physiological context to consider for the rational design of new drugs. Graphical Abstract

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Structural characterization of a novel peptide with antimicrobial activity from the venom gland of the scorpion Tityus stigmurus: Stigmurin

Cited by 51 publications

References 82 publications

Insights into Antimicrobial Peptides from Spiders and Scorpions

Insights into Antimicrobial Peptides from Spiders and Scorpions

Natural Products as Antiparasitic, Antifungal, and Antibacterial Agents

A Critical Review of Short Antimicrobial Peptides from Scorpion Venoms, Their Physicochemical Attributes, and Potential for the Development of New Drugs

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