Structural characterization of a galectin isolated from the marine sponge Chondrilla caribensis with leishmanicidal potential

Sousa, Andressa Rocha de Oliveira; Andrade, Francisco Regivânio Nascimento; Chaves, Renata Pinheiro; Sousa, Bruno; Lima, Dimas Batista de; Souza, Racquel Oliveira da Silva; Silva, Cláudio Gleidiston Lima da; Teixeira, Claudener S.; Sampaio, Alexandre Holanda; Nagano, Celso Shiniti; Carneiro, Rômulo Farias

doi:10.1016/j.bbagen.2021.129992

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…However, only a few lectins from these organisms have had their primary structures fully determined. In fact, the last sponge lectins to have its primary structure deposited were H-3 from Haliclona carulea and CCL from Chondrilla caribensis (Carneiro et al 2013a, Sousa et al 2021.…”

Section: Discussionmentioning

confidence: 99%

New lectin isolated from the tropical sponge Haliclona (Reniera) implexiformis (Hechtel, 1965) shows antibiofilm effect

Andrade

TABOSA

Torres

et al. 2023

An. Acad. Bras. Ciênc.

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A lectin from the marine sponge Haliclona (Reniera) implexiformis (HiL) was isolated by affinity chromatography on Sepharose™ matrix. HiL showed specificity for galactose and its derivatives. The glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM) were potent inhibitors. Hemagglutinating activity of the lectin was maximal between pH 5.0 and 9.0. The lectin remained active until 60°C. The presence of CaCl 2 and EDTA did not affect the hemagglutinating activity. In SDS-PAGE, HiL showed a single band of 20 kDa under reduced conditions, whereas in the non-reducing conditions, it showed a band of 20 kDa and one additional band of 36 kDa. The average molecular mass determined by Electrospray Ionization Mass Spectrometry (ESI-MS) was 35.874 ± 2 Da in native and non-reducing conditions, whereas carboxyamidomethylatedlectin showed 18,111 Da. These data indicated that HiL consists in a dimer formed by identical subunits linked by disulfide bonds. Partial amino acid sequence of HiL was determined by mass spectrometry, and revealed that it is a new type of lectin, which showed no similarity with any protein. Secondary structure consisted of 6% α-helice, 31% β-sheet, 18% β-turn and 45% random coil. HiL showed significant reduction in the number of viable cells of Staphylococcus biofilms.

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Section: Discussionmentioning

confidence: 99%

New lectin isolated from the tropical sponge Haliclona (Reniera) implexiformis (Hechtel, 1965) shows antibiofilm effect

Andrade

TABOSA

Torres

et al. 2023

An. Acad. Bras. Ciênc.

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“…CCL causes direct structural damage to promastigote with an IC 50 value of 1.2 μM. CCL binds to the L. infantum surface glycans, lipophosphoglycan (LPG) and glycoinositol phospholipid (GIP) and induces reactive oxygen species (ROS) production to kill the parasite [ 20 ]. ConA extracted from Canavalia ensiformis displays antiprotozoal activity at 0.39 μM against L. amazonensis .…”

Section: Lectins As Inhibitorsmentioning

confidence: 99%

Lectins as the prominent potential to deliver bioactive metal nanoparticles by recognizing cell surface glycans

Bala Subramaniyan,

Veerappan

2024

Heliyon

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“…Within mammals, the galectin family encompasses 16 distinct proteins, which can be categorized into three subgroups based on their structural characteristics: single CRD prototypes (galectin-1, -2, -5, -7, -10, -11, -13, -14, -15, and -16), those with two CRDs arranged in tandem (galectin-4, -6, -8, -9, and -12), and the chimeric type (galectin-3), characterized by a single CRD in conjunction with an extended N-terminal domain rich in proline and glycine (Figure 1) [30,[33][34][35]. Moreover, investigations into non-mammalian galectins, particularly those derived from marine sponges, have unveiled novel functionalities associated with the formation of tetrameric galectin arrangements [36][37][38]. The alteration in protein structure assembly changes its activity profile, such as stability and ligand specificity [37,39].…”

Section: Galectinsmentioning

confidence: 99%

“…Moreover, investigations into non-mammalian galectins, particularly those derived from marine sponges, have unveiled novel functionalities associated with the formation of tetrameric galectin arrangements [36][37][38]. The alteration in protein structure assembly changes its activity profile, such as stability and ligand specificity [37,39].…”

Section: Galectinsmentioning

confidence: 99%

Galectins in Protozoan Parasitic Diseases: Potential Applications in Diagnostics and Therapeutics

Meira,

Silva,

Quadros

et al. 2023

Cells

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Neglected tropical diseases (NTDs) constitute a group of diseases that generally develop in tropical or subtropical climatic conditions and are related to poverty. Within the spectrum of NTDs, diseases caused by protozoa such as malaria, Chagas disease, and leishmaniasis exhibit elevated mortality rates, thereby constituting a substantial public health concern. Beyond their protozoan etiology, these NTDs share other similarities, such as the challenge of control and the lack of affordable, safe, and effective drugs. In view of the above, the need to explore novel diagnostic predictors and therapeutic targets for the treatment of these parasitic diseases is evident. In this context, galectins are attractive because they are a set of lectins bound to β-galactosides that play key roles in a variety of cellular processes, including host-parasite interaction such as adhesion and entry of parasites into the host cells, and participate in antiparasitic immunity in either a stimulatory or inhibitory manner, especially the galectins-1, -2, -3, and -9. These functions bestow upon galectins significant therapeutic prospects in the context of managing and diagnosing NTDs. Thus, the present review aims to elucidate the potential role of galectins in the diagnosis and treatment of malaria, leishmaniasis, and Chagas disease.

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Structural characterization of a galectin isolated from the marine sponge Chondrilla caribensis with leishmanicidal potential

Cited by 11 publications

References 48 publications

New lectin isolated from the tropical sponge Haliclona (Reniera) implexiformis (Hechtel, 1965) shows antibiofilm effect

New lectin isolated from the tropical sponge Haliclona (Reniera) implexiformis (Hechtel, 1965) shows antibiofilm effect

Lectins as the prominent potential to deliver bioactive metal nanoparticles by recognizing cell surface glycans

Galectins in Protozoan Parasitic Diseases: Potential Applications in Diagnostics and Therapeutics

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