Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine

Vinothkanna, Annadurai; Sathiyanarayanan, Ganesan; Balaji, Perumalsamy; Mathivanan, Krishnamurthy; Pugazhendhi, Arivalagan; Ma, Yingfei; Sathiya, S.; Ramasamy, Thirumurugan

doi:10.1016/j.ijbiomac.2021.01.117

Cited by 29 publications

(29 citation statements)

References 43 publications

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“…Both EPSs in our study showed antioxidant activity, with EPS2 achieving six times more powerful reduction of ABTS. VCEAC value of EPS2 (810 μM) corresponds to 71% of the antioxidant capacity of Vitamin C. This result is similar to the antioxidant capacity effect of EPS isolated from probiotic B. licheniformis by Vinothkanna et al [ 33 ], even at a ten-fold lower concentration (0.1 mg/mL). However, regarding superoxide radicals scavenging by EPS1 and EPS2, the difference was only about 5%, which indicates that their antioxidative activity probably targets different types of ROS.…”

Section: Discussionsupporting

confidence: 83%

“…The EPSs we have obtained are heteropolysaccharides with a substrate-dependent specific content of galactose, glucose and mannose. In the literature, B. licheniformis is distinguished by the rich spectrum of sugars in EPSs composition sometimes reaching up to eight different monosaccharides, glucuronic and galacturonic acids [ 33 , 34 ]. However, EPSs containing only a galactose/mannose/glucose combination has been found in only one other Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial EPS with antioxidant activity have been reported in many species present in probiotics and functional foods, for example, Enterococcus faecium WEFA23, L. gasseri FR4, Lactiplantibacillus plantarum JLAU103, Weissella cibaria SJ14 and Bifidobacterium bifidum WBIN03 [ 5 , 50 , 51 , 52 , 53 , 64 ]. Regarding B. licheniformis , antioxidative EPSs are also usually produced by food-derived or probiotic strains, such as probiotic B. licheniformis AG-06 from Indian polyherbal traditional medicine [ 33 ], the strain S-1 from Sichuan pickles [ 49 ], and KS-17 and KS-20 isolated from Kimchi [ 65 ]. Both EPSs in our study showed antioxidant activity, with EPS2 achieving six times more powerful reduction of ABTS.…”

Section: Discussionmentioning

confidence: 99%

“…B. licheniformis is widely used in industry, horticulture and pharmacy. It is a promising producer of enzymes, platform chemicals [ 28 , 29 , 30 ], antibiotics [ 31 , 32 ] and EPSs with antimicrobial, antioxidant and anticancer activities [ 33 , 34 ]. That is why the species is commonly used in the biocontrol of plant diseases [ 35 ] and the development of probiotics [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

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New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity

et al. 2021

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Bacillus licheniformis is a soil bacterium with many industrial applications. In addition to enzymes, platform chemicals, antibiotics and phytohormones, the species produces exopolysaccharides (EPSs) of various biological activities. This study revealed that Bulgarian isolate B. licheniformis 24 produced EPSs consisting of galactose, glucose and mannose with substrate-dependent ratio. From glucose, B. licheniformis 24 secreted EPS1, consisting of 54% galactose, 39% glucose and 7% mannose. From fructose, the strain formed EPS2, containing 51% glucose, 30% mannose and 19% galactose. Batch cultivation in flasks yielded 2.2–2.6 g/L EPS1 and 1.90–2.11 g/L EPS2. Four to five times higher yields of EPS were obtained from both substrates during batch and fed-batch processes in a fermenter at 37.8 °C, pH 6.2 and aeration 3.68 vvm. The batch process with 200 g/L of starting substrates received 9.64 g/L EPS1 and 6.29 g/L EPS2, reaching maximum values at the 33rd and 24th h, respectively. Fed-batch fermentation resulted in the highest yields, 12.61 g/L EPS1 and 7.03 g/L EPS2. In all processes, EPSs were produced only in the exponential growth phase. Both EPSs exhibited antioxidant activity, but EPS2 was much more potent in this regard, reaching 811 μM Vitamin C Equivalent Antioxidant Capacity (versus 135 μM for EPS1). EPS1 displayed antibacterial activity against a non-O1 strain of Vibrio cholerae.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity

et al. 2021

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“…Genera Bacillus, Lactobacillus, and Enterococcus could be significant bacterial bio- usually aerobic bacteria and can form dormant spores under adverse environment (Nakagawa et al, 2003). Bacillus are currently used as probiotic organisms (Duc et al, 2004;Schultz et al, 2017;Vinothkanna et al, 2021). Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and fungal communities of traditional fermented Chinese soybean paste (Doujiang) and their properties

Ren

Yan

Liu

et al. 2021

Food Science & Nutrition

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In vivo functional effects of Weissella confusa VP30 exopolysaccharides on loperamide-induced constipation in rats

Park¹,

Lee

Yang³

et al. 2022

Food Sci Biotechnol

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In our previous work, we characterized the in vitro biofunctionality and evaluated the biosafety of exopolysaccharide (EPS)-producing Weissella confusa VP30 (VP30), newly isolated from healthy children's feces.Since the puri ed EPS (pEPS) from fermented milk with VP30 (VP30-EPS) showed signi cant water holding capacity, it was theorized that its consumption would relieve constipation. In this work, the in vivo functionalities of VP30-EPS and pEPS were evaluated for their effect on constipation using an experimental constipated rat model. Rats were randomly divided into four groups: (i) negative control (PBS administered normal group), (ii) loperamide treated positive control (constipation group), (iii) constipation with loperamide plus VP30-EPS (1 g/kg) and (iv) constipation with loperamide plus pEPS (0.6 g/kg) groups. Loperamide treatment induced animal constipation and signi cantly reduced the frequency of defecation, intestinal transit ratio, and water content of feces. However, all four fecal parameters were improved in both the loperamide plus VP30-EPS and pEPS administered groups as compared to the loperamide treated positive control group. No signi cant changes in dietary intake or serum hepatocellular necrosis maker levels were observed in any experimental group. These results suggest that the addition of VP30-EPS or pEPS potentially improves the functional laxative effects of commercial products. No other published research relating in vivo functional effects of EPS from Weissella spp. on constipation could be identi ed. This study suggests the possibility that VP30-EPS and pEPS can be applied to fermented and/or functional foods to relieve constipation.

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Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine

Cited by 29 publications

References 43 publications

New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity

New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity

Bacterial and fungal communities of traditional fermented Chinese soybean paste (Doujiang) and their properties

In vivo functional effects of Weissella confusa VP30 exopolysaccharides on loperamide-induced constipation in rats

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