Structural characterization by both positive and negative electrospray ion trap mass spectrometry of oligogalacturonates purified by high-performance anion-exchange chromatography

Quéméner, Bernard; Désiré, Cédric; Debrauwer, Laurent; Rathahao, Estelle

doi:10.1016/s0021-9673(02)01729-6

Cited by 26 publications

(45 citation statements)

References 25 publications

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“…18 O/ 16 O-Labeling N-Glycan Mixture Preparation N-Glycans were separated from peptides by reverse-phase liquid chromatography. Each of the PNGase F-digested samples was loaded onto a Sep-Pak C18 column, which had been pre-equilibrated in 5% acetic acid, and the Nglycans were eluted from the column with 4 mL 5% acetic acid.…”

Section: N-glycan Isolation Andmentioning

confidence: 99%

“…Each of the PNGase F-digested samples was loaded onto a Sep-Pak C18 column, which had been pre-equilibrated in 5% acetic acid, and the Nglycans were eluted from the column with 4 mL 5% acetic acid. The following 18 O-labeled/ 16 O-N-glycan mixtures at various ratios of 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, and 1:10 were prepared for N-glycans from the F samples. For N-glycans from the human serum samples, 18 O-labeled/ 16 O-N-glycans were mixed at a ratio of 1:1.…”

Section: N-glycan Isolation Andmentioning

confidence: 99%

“…The following 18 O-labeled/ 16 O-N-glycan mixtures at various ratios of 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, and 1:10 were prepared for N-glycans from the F samples. For N-glycans from the human serum samples, 18 O-labeled/ 16 O-N-glycans were mixed at a ratio of 1:1. The solutions of mixture were frozen immediately with dry ice and acetone and lyophilized to dryness.…”

Section: N-glycan Isolation Andmentioning

confidence: 99%

“…15,16 Here, we describe a simple isotope-labeling procedure to incorporate 18 O-labeling into the reducing end of Nglycans during peptide-N4-(N-acetyl-␤-glucosaminyl) asparagine amidase F (PNGase F) release. This procedure gives a 2-Da mass shift for the heavily labeled species and can be used for N-glycan relative quantification without altering the normal sample workflow.…”

mentioning

confidence: 99%

“…This procedure gives a 2-Da mass shift for the heavily labeled species and can be used for N-glycan relative quantification without altering the normal sample workflow. Furthermore, a new mathematical calculation method is described to determine the 18 O/ 16 O ratios from isotopic peaks. Lastly, this method was used in the analysis of several standard glycoproteins and human serum, which demonstrated that this method can facilitate the relative quantitation of N-glycans over a linear dynamic range of two orders, with high accuracy and reproducibility.…”

mentioning

confidence: 99%

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A Novel Method for Relative Quantitation of N-Glycans by Isotopic Labeling Using 18O-Water

Tao

Orlando

2014

J Biomol Tech

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Quantitation is an essential aspect of comprehensive glycomics study. Here, a novel isotopic-labeling method is described for N-glycan quantitation using 18 O-water. The incorporation of the 18 O-labeling into the reducing end of N-glycans is simply and efficiently achieved during peptide-N4-(N-acetyl-␤-glucosaminyl) asparagine amidase F release. This process provides a 2-Da mass difference compared with the N-glycans released in 16 O-water. A mathematical calculation method was also developed to determine the 18 O/ 16 O ratios from isotopic peaks. Application of this method to several standard glycoprotein mixtures and human serum demonstrated that this method can facilitate the relative quantitation of N-glycans over a linear dynamic range of two orders, with high accuracy and reproducibility.

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Section: N-glycan Isolation Andmentioning

confidence: 99%

Section: N-glycan Isolation Andmentioning

confidence: 99%

Section: N-glycan Isolation Andmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Novel Method for Relative Quantitation of N-Glycans by Isotopic Labeling Using 18O-Water

Tao

Orlando

2014

J Biomol Tech

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Current literature in mass spectrometry

2003

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (5 Weeks journals ‐ Search completed at 19th. February. 2003)

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Assignment of acetyl groups to O‐2 and/or O‐3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

et al. 2003

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Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

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Structural characterization by both positive and negative electrospray ion trap mass spectrometry of oligogalacturonates purified by high-performance anion-exchange chromatography

Cited by 26 publications

References 25 publications

A Novel Method for Relative Quantitation of N-Glycans by Isotopic Labeling Using 18O-Water

A Novel Method for Relative Quantitation of N-Glycans by Isotopic Labeling Using 18O-Water

Current literature in mass spectrometry

Assignment of acetyl groups to O‐2 and/or O‐3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

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