Structural characterization, antioxidant and antibacterial activities of a novel polysaccharide from Periploca laevigata root barks

Hajji, Mohamed; Hamdi, Marwa; Sellimi, Sabrine; Ksouda, Ghada; Laouer, Hocine; Li, Suming; Nasri, Monçef

doi:10.1016/j.carbpol.2018.11.020

Cited by 89 publications

(42 citation statements)

References 59 publications

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“…ROS are usually produced by cellular aerobic metabolism and can increase dramatically when the organisms are exposed to environmental stress. Several lines of evidence have suggested that polysaccharides derived from different types of plants that exhibit antioxidant effects [38,39]. The results from in vitro experiments suggested that WGP might possess antioxidant effects.…”

Section: Antioxidant Effect Of Wgp On Oxidative Stress-induced C Elementioning

confidence: 99%

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

et al. 2020

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Panax ginseng (P. ginseng) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their biological effects have not been widely studied. In this study, white ginseng neutral polysaccharide (WGNP) and white ginseng acidic polysaccharide (WGAP) fractions were purified from P. ginseng roots. The chemical properties of WGNP and WGAP were investigated using various chromatography and spectroscopy techniques, including high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and high-performance liquid chromatography with an ultra-violet detector. The antioxidant, anti-radical, and hydrogen peroxide scavenging activities were evaluated in vitro and in vivo using Caenorhabditis elegans as the model organism. Our in vitro data by ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), reducing power, ferrous ion chelating, and hydroxyl radical scavenging activity suggested that the WGAP with significantly higher uronic acid content and higher molecular weight exhibits a much stronger antioxidant effect as compared to that of WGNP. Similar antioxidant activity of WGAP was also confirmed in vivo by evaluating internal reactive oxygen species (ROS) concentration and lipid peroxidation. In conclusion, WGAP may be used as a natural antioxidant with potent scavenging and metal chelation properties.

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Section: Antioxidant Effect Of Wgp On Oxidative Stress-induced C Elementioning

confidence: 99%

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

et al. 2020

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“…The peak at 2932 cm −1 belonged to methylene C−H anti‐symmetric stretching, which was characteristic to carbohydrates [19] . The peaks at 1743 cm −1 and 1625 cm −1 represented the stretching vibration of the C=O bond [20] . The absorption peaks at 1418 cm −1 , 1371 cm −1 and 1331 cm −1 represented to the C−H bending vibration caused by the methyl groups and the methylene groups [21] .…”

Section: Resultsmentioning

confidence: 97%

Optimization of Extraction and Purification of Polysaccharides from Veronicastrum axillare, and Evaluation of Their Biological Activities

Ren

Zhang

et al. 2021

Chemistry & Biodiversity

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Veronicastrum axillare polysaccharides (VAP) were isolated by cellulase‐assisted digestion. The optimum conditions (2 % cellulase, 47 °C for 2.5 h, then, 95 °C for 2.5 h, pH 4.1, solid/liquid ratio 1 : 7.6) were identified by a combination of single factor optimization and response surface DOE (design of experiment) methods, and achieved a yield of 4.7 %. Treatment with 1 % TCA for 10 min, then, 2 % DEAE‐cellulose removed protein and colored impurities. Purified VAP retained most of the radical‐scavenging activities and GES‐1 cell protection capability in vitro, indicating VAP were the key active components of V. axillare. Some molecular features were identified by FT‐IR and NMR analyses. The molecular weight was estimated from DOSY NMR experiments to be around 21 kDa. There were 6.3 % uronic acid residues in the VAP. The constituent sugars after TFA hydrolysis were identified by HPLC to include glucose, arabinose, rhamnose, galactose, and xylose in a molar ratio of 405 : 259 : 82 : 42 : 1.

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“…A novel polysaccharide (PLP1) from P. laevigata root bark displayed antioxidant activity in a dose-dependent manner and showed strong antibacterial activity against several Gram (+) and Gram (−) strains. Hence, it was shown to be a promising source for polysaccharides in the medical and food industries [176]. The polysaccharides isolated from P. angustifolia (PAPS) were shown to decrease the rates of lipid peroxidation and protein glycation in vitro.…”

Section: Biological Activitiesmentioning

confidence: 99%

Genus Periploca (Apocynaceae): A Review of Its Classification, Phytochemistry, Biological Activities and Toxicology

et al. 2019

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The genus Periploca belongs to the family Apocynaceae, which is composed of approximately ten species of plants according to incomplete statistics. Most of these plants serve as folk medicines with a long history, especially Periploca sepium and Periploca forrestii. The botanical classifications, chemical constituents, biological activities and toxicities of the genus Periploca were summarized in the literature from 1897 to early 2019. Though the botanical classification of this genus is controversial, these species are well-known to be rich sources of diverse and complex natural products—above all, cardiac steroids and C21 pregnane steroids with special structures and obvious pharmacological activities. The various crude extracts and 314 isolated metabolites from this genus have attracted much attention in intensive biological studies, indicating that they are equipped with cardiotonic, anti-inflammatory, immunosuppressive, antitumor, antimicrobial, antioxidant, insecticidal and other properties. It is noteworthy that some cardiac glycosides showed hepatotoxicity and cardiotoxicity at certain doses. Therefore, in view of the medical and agricultural value of the genus Periploca, in-depth investigations of the pharmacology in vivo, the mechanisms of biological actions, and the pharmacokinetics of the active ingredients should be carried out in the future. Moreover, in order to ensure the safety of clinical medication, the potential toxicities of cardiac glycosides or other compounds should also be paid attention. This systematic review provides an important reference base for applied research on pharmaceuticals and pesticides from this genus.

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Structural characterization, antioxidant and antibacterial activities of a novel polysaccharide from Periploca laevigata root barks

Cited by 89 publications

References 59 publications

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

Optimization of Extraction and Purification of Polysaccharides from Veronicastrum axillare, and Evaluation of Their Biological Activities

Genus Periploca (Apocynaceae): A Review of Its Classification, Phytochemistry, Biological Activities and Toxicology

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